Mechanism and function of gasdermin-induced inflammatory cell death

Project Information

InflamCellDeath

Grant agreement ID: 770988

DOI 10.3030/770988

Project closed

EC signature date 21 February 2018

Start date 1 March 2018

End date 29 February 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 999 176,00

EU contribution € 1 999 176,00

Coordinated by

UNIVERSITE DE LAUSANNE
Switzerland

Publications

Peer reviewed articles (22)

Structural basis of NINJ1-mediated plasma membrane rupture in cell death

Author(s): Morris Degen, José Carlos Santos, Kristyna Pluhackova, Gonzalo Cebrero, Saray Ramos, Gytis Jankevicius, Ella Hartenian, Undina Guillerm, Stefania A. Mari, Bastian Kohl, Daniel J. Müller, Paul Schanda, Timm Maier, Camilo Perez, Christian Sieben, Petr Broz & Sebastian Hiller
Published in: Nature, Issue 618,, 2023, Page(s) pages 1065–1071, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-023-05991-z

Regulation of Lytic and Non-Lytic Functions of Gasdermin Pores

Author(s): Sebastian Rühl 1 , Petr Broz 2
Published in: JMB, Issue 00222836, 2022, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2021.167246

Beyond inflammasomes: emerging function of gasdermins during apoptosis and NETosis

Author(s): Kaiwen W Chen, Benjamin Demarco, Petr Broz
Published in: The EMBO Journal, Issue 39/2, 2020, Page(s) -, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.2019103397

RIPK1 activates distinct gasdermins in macrophages and neutrophils upon pathogen blockade of innate immune signaling

Author(s): Chen K.W., Demarco B., Ramos S., Heilig R., Goris M., Grayczyk J.P., Assenmacher C.A., Radaelli E., Joannas L.D., Henao-Mejia J., Tacchini-Cottier F., Brodsky I.E., Broz P.
Published in: PNAS, Issue 10916490, 2021, ISSN 1091-6490
Publisher: Proceedings of the National Academy of Sciences of the United States of America
DOI: 10.1073/pnas.2101189118

Unconventional protein secretion by gasdermin pores

Author(s): P Broz
Published in: Seminars in Immunology, Issue 69, 2023, Page(s) 101811, ISSN 1044-5323
Publisher: Academic Press
DOI: 10.1016/j.smim.2023.101811

NINJ1 induces plasma membrane rupture and release of damage-associated molecular pattern molecules during ferroptosis

Author(s): Ramos S., Hartenian E., Santos J.C., Walch P., Broz P.
Published in: The EMBO journal, Issue 43, 2024, Page(s) 7, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.1038/s44318-024-00055-y

Gasdermin D is the only Gasdermin that provides protection against acute Salmonella gut infection in mice

Author(s): Fattinger S.A., Maurer L., Geiser P., Bernard E.M., Enz U., Ganguillet S., Gül E., Kroon S., Demarco B., Mack V., Furter M., Barthel M., Pelczar P., Shao F., Broz P., Sellin M.E., Hardt W.D.
Published in: PNAS, Issue 120, 2023, Page(s) 48, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2315503120

Human GBP1 binds LPS to initiate assembly of a caspase-4 activating platform on cytosolic bacteria

Author(s): Santos, José Carlos; Boucher, Dave; Schneider, Larisa Kapinos; Demarco, Benjamin; Dilucca, Marisa; Shkarina, Kateryna; Heilig, Rosalie; Chen, Kaiwen W.; Lim, Roderick Y. H.; Broz, Petr
Published in: Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020), Issue 11, 2020, Page(s) 1, ISSN 2041-1723
Publisher: Nature Publishing Group

Selective induction of programmed cell death using synthetic biology tools

Author(s): Shkarina K., Broz P.
Published in: Seminars in cell & developmental biology, Issue 156, 2024, Page(s) 74-92, ISSN 1084-9521
Publisher: Academic Press
DOI: 10.1016/j.semcdb.2023.07.012

ESCRT-dependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation

Author(s): Sebastian Rühl, Kateryna Shkarina, Benjamin Demarco, Rosalie Heilig, José Carlos Santos, Petr Broz
Published in: Science, Issue 362/6417, 2018, Page(s) 956-960, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aar7607

Pannexin-1 channels bridge apoptosis to NLRP3 inflammasome activation

Author(s): Benjamin Demarco, Kaiwen W. Chen, Petr Broz
Published in: Molecular & Cellular Oncology, Issue 6/4, 2019, Page(s) 1610324, ISSN 2372-3556
Publisher: Taylor & Francis Online
DOI: 10.1080/23723556.2019.1610324

Pannexin‐1 promotes NLRP3 activation during apoptosis but is dispensable for canonical or noncanonical inflammasome activation

Author(s): Kaiwen W. Chen, Benjamin Demarco, Petr Broz
Published in: European Journal of Immunology, 2019, ISSN 0014-2980
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/eji.201948254

Extrinsic and intrinsic apoptosis activate pannexin‐1 to drive NLRP 3 inflammasome assembly

Author(s): Kaiwen W Chen, Benjamin Demarco, Rosalie Heilig, Kateryna Shkarina, Andreas Boettcher, Christopher J Farady, Pawel Pelczar, Petr Broz
Published in: The EMBO Journal, Issue 38/10, 2019, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.2019101638

Genetic targeting of Card19 is linked to disrupted NINJ1 expression, impaired cell lysis, and increased susceptibility to Yersinia infection

Author(s): Elisabet Bjanes 1 2 , Reyna Garcia Sillas 2 , Rina Matsuda 1 2 , Benjamin Demarco 3 , Timothée Fettrelet 3 , Alexandra A DeLaney 1 2 , Opher S Kornfeld 4 , Bettina L Lee 4 , Eric M Rodríguez López 2 5 , Daniel Grubaugh 2 , Meghan A Wynosky-Dolfi 2 , Naomi H Philip 2 5 , Elise Krespan 2 6 , Dorothy Tovar 2 , Leonel Joannas 2 7 , Daniel P Beiting 2 6 8 , Jorge He
Published in: Plos Pathogens, Issue 15537366, 2021, ISSN 1553-7366
Publisher: Public Library of Science
DOI: 10.1371/journal.ppat.1009967

The gasdermins, a protein family executing cell death and inflammation

Author(s): Petr Broz, Pablo Pelegrín, Feng Shao
Published in: Nature Reviews Immunology, Issue 20/3, 2020, Page(s) 143-157, ISSN 1474-1733
Publisher: Nature Publishing Group
DOI: 10.1038/s41577-019-0228-2

Noncanonical inflammasome signaling elicits gasdermin D–dependent neutrophil extracellular traps

Author(s): Kaiwen W. Chen, Mercedes Monteleone, Dave Boucher, Gabriel Sollberger, Divya Ramnath, Nicholas D. Condon, Jessica B. von Pein, Petr Broz, Matthew J. Sweet, Kate Schroder
Published in: Science Immunology, Issue 3/26, 2018, Page(s) eaar6676, ISSN 2470-9468
Publisher: Science Immunology
DOI: 10.1126/sciimmunol.aar6676

Cross talk between intracellular pathogens and cell death

Author(s): Benjamin Demarco, Kaiwen W. Chen, Petr Broz
Published in: Immunological Reviews, Issue 297/1, 2020, Page(s) 174-193, ISSN 0105-2896
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/imr.12892

Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality

Author(s): Benjamin Demarco, James P. Grayczyk, Elisabet Bjanes, Didier Le Roy, Wulf Tonnus, Charles-Antoine Assenmacher, Enrico Radaelli, Timothée Fettrelet, Vanessa Mack, Andreas Linkermann, Thierry Roger, Igor E. Brodsky, Kaiwen W. Chen, Petr Broz
Published in: Science Advances, Issue 6/47, 2020, Page(s) eabc3465, ISSN 2375-2548
Publisher: Science
DOI: 10.1126/sciadv.abc3465

Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

Author(s): Rosalie Heilig, Marisa Dilucca, Dave Boucher, Kaiwen W Chen, Dora Hancz, Benjamin Demarco, Kateryna Shkarina, Petr Broz
Published in: Life Science Alliance, Issue 3/6, 2020, Page(s) e202000735, ISSN 2575-1077
Publisher: Life Science Alliance
DOI: 10.26508/lsa.202000735

Optogenetic activators of apoptosis, necroptosis, and pyroptosis

Author(s): Shkarina K., Hasel de Carvalho E., Santos J.C., Ramos S., Leptin M., Broz P.
Published in: JCB, Issue 00219525, 2022, ISSN 0021-9525
Publisher: Rockefeller University Press
DOI: 10.1083/jcb.202109038

The Opto-inflammasome in zebrafish as a tool to study cell and tissue responses to speck formation and cell death.

Author(s): Hasel de Carvalho E., Dharmadhikari S.S., Shkarina K., Xiong J.R., Reversade B., Broz P., Leptin M.
Published in: eLife, Issue 2023, 2023, Page(s) e86373, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.86373

Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines.

Author(s): Shkarina K., Broz P.
Published in: Bio-protocol, Issue n.a., 2023, Page(s) n.a., ISSN 2331-8325
Publisher: Bio-protocol LLC
DOI: 10.21769/bioprotoc.4762

Non-peer reviewed articles (2)

Active membrane rupture spurs a range of cell deaths

Author(s): S. Hiller, P. Broz,
Published in: Nature, Issue 00280836, 2021, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/d41586-021-00297-4

Divide to conquer: NLRP3 is activated on dispersed trans-Golgi network

Author(s): Kaiwen W. Chen, Dave Boucher, Petr Broz
Published in: Cell Research, Issue 29/3, 2019, Page(s) 181-182, ISSN 1001-0602
Publisher: Kexue Chubaneshe/Science Press
DOI: 10.1038/s41422-018-0138-z