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Physiologically Anchored Tools for Realistic nanOmateriaL hazard aSsessment

Deliverables

Critical toxicity data for IVIVE based on existing in vivo sub-acute and (sub)chronic oral and inhalation studies

A written report of tabulated data collected from a variety of sources as described in Task 2.1.

Report on reproducibility, transferability & end-user feedback on at least one harmonised advanced in vitro lung model

SOPs for harmonized models will be reported together with reproducibility transferability and enduser feed back report

Advanced long term exposure ecotoxicity bioassays for a variety of species across a food chain

A report detailing the advanced ecotoxicity bioassays for algae, daphnids and zebrafish embryos accounting explicitly for long-term environmentally relevant ENM exposure

Materials acquisition, physicochemical intrinsic properties and endotoxin evaluation on Tier 1 and 2 ENM

A report summarizing the key features of the ENM sub-sampled or newly synthesized material sub-samples obtained from JRC-Ispra and the Fraunhofer Institute. This will include detail of each ENMs intrinsic physico-chemical characteristics and their endotoxin contamination status.

Read across for conservancy of effects of ENM across divergent taxa

A report summarizing interspeciesnanoread across modeling based on predicting toxic effects across species when the data on common MoA is available in a tested species under the realistic exposure scenarios

ENM dispersibility and physicochemical exposure relevant properties

Report detailing the physico-chemical characteristics of each ENM in relevant liquid media (corresponding to the applied experimental conditions) after dispersion using the harmonised probe-dispersion protocol developed in NANoREG.

Report on reproducibility, transferability & end-user feedback on at least one harmonised advanced GIT and liver model

SOPs for harmonized models will be reported together with reproducibility transferability and enduser feed back report

Experimental determination of the (long-term) fate of ENM in advanced lung, GIT and liver cell models

Report summarizing the interaction of the Tier 1 ENM with the lung, gut and liver models from WP3-5. This will include ENM uptake, intra-cellular fate and translocation across the cellular membrane within each test system.

Effects of subchronic oral exposure to ENM

A report that will summarize newly derived data from tissues of existing studies as well as one new 90-day oral study according to OECD TG 408.

Biodistribution of ENM after oral and inhalation exposure

A report presenting newly generated data on biodistribution and organ burdens of ENM after single and repeated exposure that will be used in WP6 as well as WP3 and WP4 to define concentrations in in vitro studies

Effects of repeated dose inhalation studies with ENM

A report that will summarize newly derived data generated from existing stored tissues obtained through previously conducted and on-going studies.

In vivo dosimetry modelling and experimental design report

Report summarizing the exposure regimen for the selected ENM in each in vivo system based on several combined dosimetry models to facilitate consideration of comparable doses in the in vitro experiments

Environmental fate and dosimetry in relevant environmental compartments

Summary report detailing the fate and dosimetry of Tier 1 ENM in environmental matrices (water and sediment based systems including test organims) as defined in WP5.

SOPs Handbook describing test methods which could be taken up by ISO and OECD

A report collating all PATROLS SOPs into a handbook that will be available for free download ebook

Report on reproducibility and transferability of harmonised advanced algal and daphnid ecotoxicity test systems

SOPs for harmonized ecotoxicity model will be reported together with reproducibility transferability and enduser feed back report

Identification of key events with predictive value for effects due to chronic ENM exposure

Report on the KEs/pathways of developed AOPs based on established and putative AOPs, published toxicity data and meta-analyses with the aim of enabling WP3 and 4 to develop targeted in vitro bioassays with enhanced predictive value. The report will also provide data addressing knowledge gaps based on existing data and AOPs.

Compare and contrast advanced human lung models to cross-species models

Report summarizing the comparison between toxicity endpoint data andor AOPs data generated across species in WP5 Task 53 with lung model data

Management framework & shared server for information management established

A report summarizing the management tools provided to facilitate the day-day project coordination.

Environmental dosimetry modelling and dose-response prediction report

Report detailing the outputs from modelling bioaccumulation data and ENM redistribution kinetics across the ecological taxa assessed

Compare and contrast 3D GIT and liver models to cross-species models

Report summerizing the comparison between toxicity endpoint data and/or AOPs data generated across species in WP5 (Task 5.3) with GIT and liver model data.

Guidance document for the design of a large-scale multi-centre ring test

Guidance document required for future validation of the test methods developed

Physicochemical hazard concern phenomena, with specific focus on dissolution and hydrochemical reactivity

Report providing an overview of the dissolution rates redox and photocatalytic activity radical formation capacity of the selected ENM in standard media as well as in specific in vitro and simulant biological media

Report detailing IVIVE models

Summary report specifiying the IVIVE models developed including uncertainty factors regarding interanimal and interspecies differences

In vitro dosimetry modelling and experimental design report

Report summarizing the exposure regimen for the selected ENM in each in vitro system based on several combined dosimetry models.

PATROLS MODA

Report on the developed MODA for WP6.

SOPs on programming codes

Report providing SOPs on the programming codes An appendix containing the background data generated whilst developing the SOP will be included

SOPs for ENM characterisation, reactivity and behaviour in biological matrices

This report will consist of the SOPs developed through Task 14 and 15 for fate and dose characterization An appendix containing the background data generated whilst developing the SOP will be included

Six-monthly E-newsletters documenting updates

Electronic newsletters will be prepared every 6-months for dissemination of PATROLS achievements and updates through a wide contacts distribution list.

PATROLS database

A comprehensive project database system containing the physicochemical toxicological and other related data generated by WP15 as well as in vivo data collected from other sources

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Publications

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Author(s): Samantha V. Llewellyn, Gillian E. Conway, Ume-Kulsoom Shah, Stephen J. Evans, Gareth J.S. Jenkins, Martin J.D. Clift, Shareen H. Doak
Published in: Journal of Visualized Experiments, 160, 2020, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/61141

The influence of exposure approaches to <i>in vitro</i> lung epithelial barrier models to assess engineered nanomaterial hazard

Author(s): Meldrum, Kirsty; Evans, Stephen J.; Vogel, Ulla; Tran, Lang; Doak, Shareen H.; Clift, Martin J. D.
Published in: Nanotoxicology, 1, 2022, ISSN 1743-5390
Publisher: Informa Healthcare
DOI: 10.1080/17435390.2022.2051627

Translating Scientific Advances in the AOP Framework to Decision Making for Nanomaterials

Author(s): James D. Ede, Vladimir Lobaskin, Ulla Vogel, Iseult Lynch, Sabina Halappanavar, Shareen H. Doak, Megan G. Roberts, Jo Anne Shatkin
Published in: Nanomaterials, 10/6, 2020, Page(s) 1229, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano10061229

Optimization of an air-liquid interface in vitro cell co-culture model to estimate the hazard of aerosol exposures

Author(s): Rui-Wen He, Hedwig M. Braakhuis, Rob J. Vandebriel, Yvonne C.M. Staal, Eric R. Gremmer, Paul H.B. Fokkens, Claudia Kemp, Jolanda Vermeulen, Remco H.S. Westerink, Flemming R. Cassee
Published in: Journal of Aerosol Science, 153, 2021, Page(s) 105703, ISSN 0021-8502
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.jaerosci.2020.105703

Breathing in vitro: Designs and applications of engineered lung models

Author(s): Roberta Nossa, Joana Costa, Ludovica Cacopardo, Arti Ahluwalia
Published in: Journal of Tissue Engineering, 12, 2021, Page(s) 204173142110086, ISSN 2041-7314
Publisher: Sage Publishing
DOI: 10.1177/20417314211008696

Oxidative stress actuated by cellulose nanocrystals and nanofibrils in aquatic organisms of different trophic levels

Author(s): Zhuang Wang, Lan Song, Nan Ye, Qi Yu, Yujia Zhai, Fan Zhang, Martina G. Vijver, Willie J.G.M. Peijnenburg
Published in: NanoImpact, 17, 2020, Page(s) 100211, ISSN 2452-0748
Publisher: Elsevier
DOI: 10.1016/j.impact.2020.100211

The Road to Achieving the European Commission's Chemicals Strategy for Nanomaterial Sustainability—A PATROLS Perspective on New Approach Methodologies

Author(s): Doak, Shareen H.; Clift, Martin J. D.; Costa, Anna; Delmaar, Christiaan; Gosens, Ilse; Halappanavar, Sabina; Kelly, Sean; Pejinenburg, Willie J. G. M.; Rothen‐Rutishauser, Barbara; Schins, Roel P. F.; Stone, Vicki; Tran, Lang; Vijver, Martina G.; Vogel, Ulla; Wohlleben, Wendel; Cassee, Flemming R.
Published in: SMall, 1, 2022, ISSN 1613-6829
Publisher: Wiley
DOI: 10.1002/smll.202200231

Adverse Outcome Pathway Development for Assessment of Lung Carcinogenicity by Nanoparticles

Author(s): Penny Nymark, Hanna L. Karlsson, Sabina Halappanavar, Ulla Vogel
Published in: Frontiers in Toxicology, 3, 2021, ISSN 2673-3080
Publisher: Frontiers Media SA
DOI: 10.3389/ftox.2021.653386

Effects of humic substances on the aqueous stability of cerium dioxide nanoparticles and their toxicity to aquatic organisms

Author(s): Qi Yu, Zhuang Wang, Yujia Zhai, Fan Zhang, Martina G. Vijver, Willie J.G.M. Peijnenburg
Published in: Science of The Total Environment, 781, 2021, Page(s) 146583, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2021.146583

Ingested Engineered Nanomaterials Affect the Expression of Mucin Genes—An In Vitro-In Vivo Comparison

Author(s): Gerrit Bredeck; Angela A. M. Kämpfer; Adriana Sofranko; Tina Wahle; Veronika Büttner; Catrin Albrecht; Roel P. F. Schins
Published in: Nanomaterials, Vol 11, Iss 2621, p 2621 (2021), 11, 2021, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano11102621

Introducing WikiPathways as a Data-Source to Support Adverse Outcome Pathways for Regulatory Risk Assessment of Chemicals and Nanomaterials

Author(s): Marvin Martens, Tim Verbruggen, Penny Nymark, Roland Grafström, Lyle D. Burgoon, Hristo Aladjov, Fernando Torres Andón, Chris T. Evelo, Egon L. Willighagen
Published in: Frontiers in Genetics, 9, 2018, ISSN 1664-8021
Publisher: Frontiers Media
DOI: 10.3389/fgene.2018.00661

Validation and Demonstration of an Atmosphere-Temperature-pH-Controlled Stirred Batch Reactor System for Determination of (Nano)Material Solubility and Dissolution Kinetics in Physiological Simulant Lung Fluids

Author(s): Holmfred, Else; Loeschner, Katrin; Sloth, Jens J.; Jensen, Keld Alstrup
Published in: Nanomaterials, vol. 12 , no. 3 , 517, 2022, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano12030517

Adverse outcome pathways as a tool for the design of testing strategies to support the safety assessment of emerging advanced materials at the nanoscale

Author(s): Sabina Halappanavar, Sybille van den Brule, Penny Nymark, Laurent Gaté, Carole Seidel, Sarah Valentino, Vadim Zhernovkov, Pernille Høgh Danielsen, Andrea De Vizcaya, Henrik Wolff, Tobias Stöger, Andrey Boyadziev, Sarah Søs Poulsen, Jorid Birkelund Sørli, Ulla Vogel
Published in: Particle and Fibre Toxicology, 17/1, 2020, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-020-00344-4

Opportunities and Challenges for Integrating New In Vitro Methodologies in Hazard Testing and Risk Assessment

Author(s): Natalie Burden, Martin J. D. Clift, Gareth J. S. Jenkins, Briony Labram, Fiona Sewell
Published in: Small, 17/15, 2021, Page(s) 2006298, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202006298

Particulate and drug-induced toxicity assessed in novel quadruple cell human primary hepatic disease models of steatosis and pre-fibrotic NASH

Author(s): Ali Kermanizadeh; Jessica Valli; Katarzyna Sanchez; Simon Hutter; Agnieszka Pawlowska; Graeme Whyte; Wolfgang Moritz; Vicki Stone
Published in: Archives of Toxicology, 7/2021, 2021, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-021-03181-2

Microbiota-dependent TLR2 signaling reduces silver nanoparticle toxicity to zebrafish larvae

Author(s): Brinkmann, Bregje W; Koch, Bjørn E V; Peijnenburg, Willie J G M; Vijver, Martina G
Published in: Ecotoxicology and Environmental Safety, 3, 2022, ISSN 0147-6513
Publisher: Academic Press
DOI: 10.1016/j.ecoenv.2022.113522

Interlaboratory comparison of an intestinal triple culture to confirm transferability and reproducibility

Author(s): Kämpfer, Angela A. M.; Shah, Ume-Kulsoom; Chu, Shui L.; Busch, Mathias; Büttner, Veronika; He, Ruiwen; Rothen-Rutishauser, Barbara; Schins, Roel P. F.; Jenkins, Gareth J.
Published in: In Vitro Models, 3, 2022, ISSN 2731-3441
Publisher: Springer
DOI: 10.1007/s44164-022-00025-w

New insights into organ-specific oxidative stress mechanisms using a novel biosensor zebrafish

Author(s): Sulayman Mourabit, Jennifer A. Fitzgerald, Robert P. Ellis, Aya Takesono, Cosima S. Porteus, Maciej Trznadel, Jeremy Metz, Matthew J. Winter, Tetsuhiro Kudoh, Charles R. Tyler
Published in: Environment International, 133, 2019, Page(s) 105138, ISSN 0160-4120
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2019.105138

Use of EpiAlveolar Lung Model to Predict Fibrotic Potential of Multiwalled Carbon Nanotubes

Author(s): Hana Barosova, Anna G. Maione, Dedy Septiadi, Monita Sharma, Laetitia Haeni, Sandor Balog, Olivia O’Connell, George R. Jackson, David Brown, Amy J. Clippinger, Patrick Hayden, Alke Petri-Fink, Vicki Stone, Barbara Rothen-Rutishauser
Published in: ACS Nano, 14/4, 2020, Page(s) 3941-3956, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.9b06860

The fate and toxicity of Pb-based perovskite nanoparticles on soil bacterial community: Impacts of pH, humic acid, and divalent cations

Author(s): Yujia Zhai, Zhuang Wang, Guiyin Wang, Willie J.G.M. Peijnenburg, Martina G. Vijver
Published in: Chemosphere, 249, 2020, Page(s) 126564, ISSN 0045-6535
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.chemosphere.2020.126564

Using AOP-Wiki to support the ecotoxicological risk assessment of nanomaterials: first steps in the development of novel adverse outcome pathways

Author(s): Karolina Jagiello; Beata Judzinska; Anita Sosnowska; Iseult Lynch; Sabina Halappanavar; Tomasz Puzyn
Published in: Environmental science: nano, 3, 2022, ISSN 2051-8161
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1en01127h

Correlation analysis of single- and multigenerational endpoints in Daphnia magna toxicity tests: A case-study using TiO2 nanoparticles

Author(s): Nederstigt, Tom A.P.; Peijnenburg, Willie J.G.M.; Blom, Rody; Vijver, Martina G.
Published in: ecotoxicology and environmental safety, 3, 2022, ISSN 0147-6513
Publisher: Academic Press
DOI: 10.1016/j.ecoenv.2022.113792

Colonizing microbiota protect zebrafish larvae against silver nanoparticle toxicity

Author(s): Bregje W. Brinkmann, Bjørn E. V. Koch, Herman P. Spaink, Willie J. G. M. Peijnenburg, Martina G. Vijver
Published in: Nanotoxicology, 14/6, 2020, Page(s) 725-739, ISSN 1743-5390
Publisher: Informa Healthcare
DOI: 10.1080/17435390.2020.1755469

Simulating Nanomaterial Transformation in Cascaded Biological Compartments to Enhance the Physiological Relevance of In Vitro Dosing Regimes: Optional or Required?

Author(s): Samantha V. Llewellyn, Angela Kämpfer, Johannes G. Keller, Klaus Vilsmeier, Veronika Büttner, Didem Ag Seleci, Roel P. F. Schins, Shareen H. Doak, Wendel Wohlleben
Published in: Small, 2021, Page(s) 2004630, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202004630

Applicability of nanomaterial-specific guidelines within long-term Daphnia magna toxicity assays: A case study on multigenerational effects of nTiO2 and nCeO2 exposure in the presence of artificial daylight

Author(s): Nederstigt, Tom A.P.; Peijnenburg, Willie J.G.M.; Bleeker, Eric A.J.; Vijver, Martina G.
Published in: Regulatory Toxicology and Pharmacology, 1, 2022, ISSN 0273-2300
Publisher: Academic Press
DOI: 10.1016/j.yrtph.2022.105156

The preparation temperature influences the physicochemical nature and activity of nanoceria

Author(s): Robert A Yokel, Wendel Wohlleben, Johannes Georg Keller, Matthew L Hancock, Jason M Unrine, D Allan Butterfield, Eric A Grulke
Published in: Beilstein Journal of Nanotechnology, 12, 2021, Page(s) 525-540, ISSN 2190-4286
Publisher: Beilstein-Institut Zur Forderung der Chemischen Wissenschaften
DOI: 10.3762/bjnano.12.43

Development of a Quasi‐QSAR Model for Prediction of the Immobilization Response of <i>Daphnia magna</i> Exposed to Metal‐Based Nanomaterials

Author(s): Bunmahotama, Warisa; Vijver, Martina G.; Peijnenburg, Willie
Published in: Environmental Toxicology and Chemistry, 1, 2022, ISSN 1552-8618
Publisher: Wiley
DOI: 10.1002/etc.5322

An Inflamed Human Alveolar Model for Testing the Efficiency of Anti-inflammatory Drugs in vitro

Author(s): Barbara Drasler, Bedia Begum Karakocak, Esma Bahar Tankus, Hana Barosova, Jun Abe, Mauro Sousa de Almeida, Alke Petri-Fink, Barbara Rothen-Rutishauser
Published in: Frontiers in Bioengineering and Biotechnology, 8, 2020, ISSN 2296-4185
Publisher: Frontiers Media SA
DOI: 10.3389/fbioe.2020.00987

Inter-laboratory variability of A549 epithelial cells grown under submerged and air-liquid interface conditions

Author(s): Hana Barosova, Kirsty Meldrum, Bedia Begum Karakocak, Sandor Balog, Shareen H. Doak, Alke Petri-Fink, Martin J.D. Clift, Barbara Rothen-Rutishauser
Published in: Toxicology in Vitro, 75, 2021, Page(s) 105178, ISSN 0887-2333
Publisher: Elsevier BV
DOI: 10.1016/j.tiv.2021.105178

Advanced In Vitro Testing Strategies and Models of the Intestine for Nanosafety Research

Author(s): Angela A. M. Kämpfer, Mathias Busch, Roel P. F. Schins
Published in: Chemical Research in Toxicology, 33/5, 2020, Page(s) 1163-1178, ISSN 0893-228X
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrestox.0c00079

Oral toxicity of titanium dioxide P25 at repeated dose 28-day and 90-day in rats

Author(s): Min Beom Heo, Minjeong Kwak, Kyu Sup An, Hye Jin Kim, Hyeon Yeol Ryu, So Min Lee, Kyung Seuk Song, In Young Kim, Ji-Hwan Kwon, Tae Geol Lee
Published in: Particle and Fibre Toxicology, 17/1, 2020, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-020-00350-6

Oxygen Consumption Characteristics in 3D Constructs Depend on Cell Density

Author(s): Chiara Magliaro, Giorgio Mattei, Flavio Iacoangeli, Alessandro Corti, Vincenzo Piemonte, Arti Ahluwalia
Published in: Frontiers in Bioengineering and Biotechnology, 7, 2019, ISSN 2296-4185
Publisher: Frontiers
DOI: 10.3389/fbioe.2019.00251

Prediction of the Joint Toxicity of Multiple Engineered Nanoparticles: The Integration of Classic Mixture Models and In Silico Methods

Author(s): Fan Zhang, Zhuang Wang, Martina G. Vijver, Willie J. G. M. Peijnenburg
Published in: Chemical Research in Toxicology, 2020, ISSN 0893-228X
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrestox.0c00300

Nanomaterials and Innate Immunity: A Perspective of the Current Status in Nanosafety

Author(s): James G. Cronin, Nicholas Jones, Catherine A. Thornton, Gareth J. S. Jenkins, Shareen H. Doak, Martin J. D. Clift
Published in: Chemical Research in Toxicology, 33/5, 2020, Page(s) 1061-1073, ISSN 0893-228X
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrestox.0c00051

Method for extraction of nanoscale plastic debris from soil

Author(s): Fazel Abdolahpur Monikh, Nikki Doornhein, Stefan Romeijn, Martina G. Vijver, Willie J. G. M. Peijnenburg
Published in: Analytical Methods, 13/13, 2021, Page(s) 1576-1583, ISSN 1759-9660
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0ay02308f

Understanding the Development, Standardization, and Validation Process of Alternative In Vitro Test Methods for Regulatory Approval from a Researcher Perspective

Author(s): Aurora Bas, Nicole Burns, Andrew Gulotta, James Junker, Barbara Drasler, Roman Lehner, Lothar Aicher, Samuel Constant, Alke Petri‐Fink, Barbara Rothen‐Rutishauser
Published in: Small, 2021, Page(s) 2006027, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202006027

A “point-of-entry” bioaccumulation study of nanoscale pigment copper phthalocyanine in aquatic organisms

Author(s): Chengfang Pang, Lars M. Skjolding, Henriette Selck, Anders Baun
Published in: Environmental Science: Nano, 8/2, 2021, Page(s) 554-564, ISSN 2051-8153
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0en00705f

Interaction between a nano-formulation of atrazine and rhizosphere bacterial communities: atrazine degradation and bacterial community alterations

Author(s): Yujia Zhai, Fazel Abdolahpur Monikh, Juan Wu, Renato Grillo, Daniel Arenas-Lago, Gopala Krishna Darbha, Martina G. Vijver, Willie J. G. M. Peijnenburg
Published in: Environmental Science: Nano, 7/11, 2020, Page(s) 3372-3384, ISSN 2051-8153
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0en00638f

The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment

Author(s): Verdon, Rachel; Stone, Vicki; Murphy, Fiona; Christopher, Emily; Johnston, Helinor; Doak, Shareen; Vogel, Ulla; Haase, Andrea; Kermanizadeh, Ali
Published in: Particle and Fibre Toxicology, 19, 2022, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-022-00476-9

Effect on Mouse Liver Morphology of CeO2, TiO2 and Carbon Black Nanoparticles Translocated from Lungs or Deposited Intravenously

Author(s): Justyna Modrzynska, Alicja Mortensen, Trine Berthing, Gitte Ravn-Haren, Józef Szarek, Anne Thoustrup Saber, Ulla Vogel
Published in: Applied Nano, 2/3, 2021, Page(s) 222-241, ISSN 2673-3501
Publisher: Applied Nano
DOI: 10.3390/applnano2030016

In Vitro Three‐Dimensional Liver Models for Nanomaterial DNA Damage Assessment

Author(s): Samantha V. Llewellyn, Marije Niemeijer, Penny Nymark, Martijn J. Moné, Bob Water, Gillian E. Conway, Gareth J. S. Jenkins, Shareen H. Doak
Published in: Small, 2021, Page(s) 2006055, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202006055

Assessment of nanomaterial-induced hepatotoxicity using a 3D human primary multi-cellular microtissue exposed repeatedly over 21 days - the suitability of the in vitro system as an in vivo surrogate

Author(s): Ali Kermanizadeh, Trine Berthing, Ewa Guzniczak, Melanie Wheeldon, Graeme Whyte, Ulla Vogel, Wolfgang Moritz, Vicki Stone
Published in: Particle and Fibre Toxicology, 16/1, 2019, ISSN 1743-8977
Publisher: BioMed Central
DOI: 10.1186/s12989-019-0326-0

Correction to: An Integrated In Vitro-In Silico Approach for Silver Nanoparticle Dosimetry in Cell Cultures.

Author(s): Poli, Daniele; Mattei, Giorgio; Ucciferri, Nadia; Ahluwalia, Arti
Published in: Annals of Biomedical Engineering, 48 (2020), 2020, Page(s) 1441, ISSN 0090-6964
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10439-020-02463-7

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

Author(s): Hana Barosova, Barbara Drasler, Alke Petri-Fink, Barbara Rothen-Rutishauser
Published in: Journal of Visualized Experiments, 159, 2020, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/61090

Impacts of a novel controlled-release TiO2-coated (nano-) formulation of carbendazim and its constituents on freshwater macroinvertebrate communities

Author(s): Nederstigt, Tom A.P.; Peijnenburg, Willie J.G.M.; Schrama, Maarten; van Ommen, J. Ruud; Vijver, Martina G.
Published in: science of the total environment, 1, 2022, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2022.156554

Compositional and predicted functional dynamics of soil bacterial community in response to single pulse and repeated dosing of titanium dioxide nanoparticles

Author(s): Yujia Zhai, Gang Liu, Thijs Bosker, Elise Baas, Willie J.G.M. Peijnenburg, Martina G. Vijver
Published in: NanoImpact, 16, 2019, Page(s) 100187, ISSN 2452-0748
Publisher: Elsevier
DOI: 10.1016/j.impact.2019.100187

Transcriptomics‐Based and AOP‐Informed Structure–Activity Relationships to Predict Pulmonary Pathology Induced by Multiwalled Carbon Nanotubes

Author(s): Karolina Jagiello, Sabina Halappanavar, Anna Rybińska‐Fryca, Andrew Willliams, Ulla Vogel, Tomasz Puzyn
Published in: Small, 2021, Page(s) 2003465, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202003465

Using 3D gastrointestinal tract in vitro models with microfold cells and mucus secreting ability to assess the hazard of copper oxide nanomaterials

Author(s): Victor C. Ude, David M. Brown, Vicki Stone, Helinor J. Johnston
Published in: Journal of Nanobiotechnology, 17/1, 2019, ISSN 1477-3155
Publisher: BioMed Central
DOI: 10.1186/s12951-019-0503-1

The importance of inter-individual Kupffer cell variability in the governance of hepatic toxicity in a 3D primary human liver microtissue model

Author(s): Ali Kermanizadeh, David M. Brown, Wolfgang Moritz, Vicki Stone
Published in: Scientific Reports, 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-43870-8

Real-time cellular impedance monitoring and imaging of biological barriers in a dual-flow membrane bioreactor

Author(s): L. Cacopardo, J. Costa, S. Giusti, L. Buoncompagni, S. Meucci, A. Corti, G. Mattei, A. Ahluwalia
Published in: Biosensors and Bioelectronics, 140, 2019, Page(s) 111340, ISSN 0956-5663
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2019.111340

Method for Extraction and Quantification of Metal-Based Nanoparticles in Biological Media: Number-Based Biodistribution and Bioconcentration

Author(s): Fazel Abdolahpur Monikh, Latifeh Chupani, Eliska Zusková, Ruud Peters, Marie Vancová, Martina G. Vijver, Petr Porcal, Willie J.G.M. Peijnenburg
Published in: Environmental Science & Technology, 53/2, 2018, Page(s) 946-953, ISSN 0013-936X
Publisher: American Chemical Society
DOI: 10.1021/acs.est.8b03715

Unveiling the Colours of Cellulose Nitrate Black and White Film-based Negatives in Colonial Photography

Author(s): Élia Roldão, A. Jorge Parola, Márcia Vilarigues, Bertrand Lavédrine, Ana Maria Ramos
Published in: Studies in Conservation, 65/2, 2020, Page(s) 118-126, ISSN 0039-3630
Publisher: Maney Publishing
DOI: 10.1080/00393630.2019.1662672

The Use of Nanomaterial In Vivo Organ Burden Data for In Vitro Dose Setting

Author(s): Lan Ma‐Hock, Ursula G. Sauer, Emmanuel Ruggiero, Johannes‐Georg Keller, Wendel Wohlleben, Robert Landsiedel
Published in: Small, 17/15, 2021, Page(s) 2005725, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202005725

An Integrated In Vitro–In Silico Approach for Silver Nanoparticle Dosimetry in Cell Cultures

Author(s): Daniele Poli, Giorgio Mattei, Nadia Ucciferri, Arti Ahluwalia
Published in: Annals of Biomedical Engineering, 48/4, 2020, Page(s) 1271-1280, ISSN 0090-6964
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10439-020-02449-5

Model Complexity as Determining Factor for In Vitro Nanosafety Studies: Effects of Silver and Titanium Dioxide Nanomaterials in Intestinal Models

Author(s): Angela A. M. Kämpfer, Mathias Busch, Veronika Büttner, Gerrit Bredeck, Burkhard Stahlmecke, Bryan Hellack, Isabelle Masson, Adriana Sofranko, Catrin Albrecht, Roel P. F. Schins
Published in: Small, Monthly, 2020, Page(s) 2004223, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202004223

Allometric Scaling of physiologically-relevant organoids

Author(s): Chiara Magliaro, Andrea Rinaldo, Arti Ahluwalia
Published in: Scientific Reports, 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-48347-2

Adaptation of the in vitro micronucleus assay for genotoxicity testing using 3D liver models supporting longer-term exposure durations

Author(s): Gillian E Conway, Ume-Kulsoom Shah, Samantha Llewellyn, Tereza Cervena, Stephen J Evans, Abdullah S Al Ali, Gareth J Jenkins, Martin J D Clift, Shareen H Doak
Published in: Mutagenesis, 35/4, 2020, Page(s) 319-330, ISSN 0267-8357
Publisher: Oxford University Press
DOI: 10.1093/mutage/geaa018

TiO2@BSA nano-composites investigated through orthogonal multi-techniques characterization platform

Author(s): Simona Ortelli, Anna L. Costa, Ilaria Zanoni, Magda Blosi, Otmar Geiss, Ivana Bianchi, Dora Mehn, Francesco Fumagalli, Giacomo Ceccone, Giuditta Guerrini, Luigi Calzolai
Published in: Colloids and Surfaces B: Biointerfaces, 207, 2021, Page(s) 112037, ISSN 0927-7765
Publisher: Elsevier BV
DOI: 10.1016/j.colsurfb.2021.112037

A Small-Scale Setup for Algal Toxicity Testing of Nanomaterials and Other Difficult Substances

Author(s): Lars Michael Skjolding, Susanne Kruse, Sara Nørgaard Sørensen, Rune Hjorth, Anders Baun
Published in: Journal of Visualized Experiments, 164, 2020, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/61209

An Alternative Perspective towards Reducing the Risk of Engineered Nanomaterials to Human Health

Author(s): Martin J. D. Clift, Gareth J. S. Jenkins, Shareen H. Doak
Published in: Small, 16/36, 2020, Page(s) 2002002, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202002002

Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota

Author(s): Bregje W. Brinkmann, Wouter F. Beijk, Redmar C. Vlieg, S. John T. van Noort, Jorge Mejia, Julien L. Colaux, Stéphane Lucas, Gerda Lamers, Willie J.G.M. Peijnenburg, Martina G. Vijver
Published in: Aquatic Toxicology, 232, 2021, Page(s) 105744, ISSN 0166-445X
Publisher: Elsevier BV
DOI: 10.1016/j.aquatox.2021.105744

Dosimetry in vitro – exploring the sensitivity of deposited dose predictions vs. affinity, polydispersity, freeze-thawing, and analytical methods

Author(s): Johannes G. Keller, Daniel F. Quevedo, Lara Faccani, Anna L. Costa, Robert Landsiedel, Kai Werle, Wendel Wohlleben
Published in: Nanotoxicology, Monthly, 2020, Page(s) 1-14, ISSN 1743-5390
Publisher: Informa Healthcare
DOI: 10.1080/17435390.2020.1836281

Time dependent impact of copper oxide nanomaterials on the expression of genes associated with oxidative stress, metal binding, inflammation and mucus secretion in single and co-culture intestinal in vitro models

Author(s): Victor C. Ude, David M. Brown, Vicki Stone, Helinor J. Johnston
Published in: Toxicology in Vitro, 74, 2021, Page(s) 105161, ISSN 0887-2333
Publisher: Elsevier BV
DOI: 10.1016/j.tiv.2021.105161

Acute hazard assessment of silver nanoparticles following intratracheal instillation, oral and intravenous injection exposures

Author(s): Kermanizadeh, Ali; Jacobsen, Nicklas R.; Mroczko, Agnieszka; Brown, David; Stone, Vicki
Published in: Nanotoxicology, 1, 2022, ISSN 1743-5390
Publisher: Informa Healthcare
DOI: 10.1080/17435390.2021.2020350

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Author(s): Hedwig M. Braakhuis, Ruiwen He, Rob J. Vandebriel, Eric R. Gremmer, Edwin Zwart, Jolanda P. Vermeulen, Paul Fokkens, John Boere, Ilse Gosens, Flemming R. Cassee
Published in: Journal of Visualized Experiments, 159, 2020, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/61210

A review of hepatic nanotoxicology - summation of recent findings and considerations for the next generation of study designs.

Author(s): Ali Kermanizadeh; Vicki Stone; Leagh G. Powell
Published in: Journal of Toxicology and Environmental Health, Part B, 1, 2020, Page(s) 137-176, ISSN 1521-6950
Publisher: Taylor & Francis Online
DOI: 10.1080/10937404.2020.1751756

Understanding the impact of more realistic low-dose, prolonged engineered nanomaterial exposure on genotoxicity using 3D models of the human liver

Author(s): Samantha V. Llewellyn, Gillian E. Conway, Ilaria Zanoni, Amalie Kofoed Jørgensen, Ume-Kulsoom Shah, Didem Ag Seleci, Johannes G. Keller, Jeong Won Kim, Wendel Wohlleben, Keld Alstrup Jensen, Anna Costa, Gareth J. S. Jenkins, Martin J. D. Clift, Shareen H. Doak
Published in: Journal of Nanobiotechnology, 19/1, 2021, ISSN 1477-3155
Publisher: BioMed Central
DOI: 10.1186/s12951-021-00938-w

The variances in cytokine production profiles from non- or activated THP-1, Kupffer cell and human blood derived primary macrophages following exposure to either alcohol or a panel of engineered nanomaterials

Author(s): Ali Kermanizadeh, David M. Brown, Vicki Stone
Published in: PLOS ONE, 14/8, 2019, Page(s) e0220974, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0220974

Colonizing microbiota protect zebrafish larvae against silver nanoparticle toxicity.

Author(s): Bjørn E. V. Koch; Willie J.G.M. Peijnenburg; Herman P. Spaink; Bregje W. Brinkmann; Martina G. Vijver
Published in: VOLUME=14;ISSUE=6;STARTPAGE=725;ENDPAGE=739;TITLE=Nanotoxicology, 12, 2020, ISSN 1040-8398
Publisher: Taylor & Francis
DOI: 10.6084/m9.figshare.12181941.v1

Additional file 1 of Understanding the impact of more realistic low-dose, prolonged engineered nanomaterial exposure on genotoxicity using 3D models of the human liver

Author(s): Llewellyn, Samantha V.; Conway, Gillian E.; Zanoni, Ilaria; Jørgensen, Amalie Kofoed; Shah, Ume-Kulsoom; Seleci, Didem Ag; Keller, Johannes G.; Kim, Jeong Won; Wohlleben, Wendel; Jensen, Keld Alstrup; Costa, Anna; Jenkins, Gareth J. S.; Clift, Martin J. D.; Doak, Shareen H.
Published in: Springer Nature, 2, 2021, Page(s) N/A, ISSN 1476-4660
Publisher: Springer
DOI: 10.6084/m9.figshare.14870570

Engineering Gels with Time-Evolving Viscoelasticity

Author(s): Giorgio Mattei, Ludovica Cacopardo, Arti Ahluwalia
Published in: Materials, 13/2, 2020, Page(s) 438, ISSN 1996-1944
Publisher: MDPI Open Access Publishing
DOI: 10.3390/ma13020438

Allometric Scaling of physiologically-relevant organoids

Author(s): Chiara Magliaro, Andrea Rinaldo, Arti Ahluwalia
Published in: BioRxiv, Regular online, 2019, ISSN 1476-4687
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/559682