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Physiologically Anchored Tools for Realistic nanOmateriaL hazard aSsessment

Deliverables

Critical toxicity data for IVIVE based on existing in vivo sub-acute and (sub)chronic oral and inhalation studies

A written report of tabulated data collected from a variety of sources as described in Task 2.1.

Advanced long term exposure ecotoxicity bioassays for a variety of species across a food chain

A report detailing the advanced ecotoxicity bioassays for algae, daphnids and zebrafish embryos accounting explicitly for long-term environmentally relevant ENM exposure

Materials acquisition, physicochemical intrinsic properties and endotoxin evaluation on Tier 1 and 2 ENM

A report summarizing the key features of the ENM sub-sampled or newly synthesized material sub-samples obtained from JRC-Ispra and the Fraunhofer Institute. This will include detail of each ENMs intrinsic physico-chemical characteristics and their endotoxin contamination status.

ENM dispersibility and physicochemical exposure relevant properties

Report detailing the physico-chemical characteristics of each ENM in relevant liquid media (corresponding to the applied experimental conditions) after dispersion using the harmonised probe-dispersion protocol developed in NANoREG.

Experimental determination of the (long-term) fate of ENM in advanced lung, GIT and liver cell models

Report summarizing the interaction of the Tier 1 ENM with the lung, gut and liver models from WP3-5. This will include ENM uptake, intra-cellular fate and translocation across the cellular membrane within each test system.

Effects of subchronic oral exposure to ENM

A report that will summarize newly derived data from tissues of existing studies as well as one new 90-day oral study according to OECD TG 408.

Effects of repeated dose inhalation studies with ENM

A report that will summarize newly derived data generated from existing stored tissues obtained through previously conducted and on-going studies.

Environmental fate and dosimetry in relevant environmental compartments

Summary report detailing the fate and dosimetry of Tier 1 ENM in environmental matrices (water and sediment based systems including test organims) as defined in WP5.

Identification of key events with predictive value for effects due to chronic ENM exposure

Report on the KEs/pathways of developed AOPs based on established and putative AOPs, published toxicity data and meta-analyses with the aim of enabling WP3 and 4 to develop targeted in vitro bioassays with enhanced predictive value. The report will also provide data addressing knowledge gaps based on existing data and AOPs.

Management framework & shared server for information management established

A report summarizing the management tools provided to facilitate the day-day project coordination.

Compare and contrast 3D GIT and liver models to cross-species models

Report summerizing the comparison between toxicity endpoint data and/or AOPs data generated across species in WP5 (Task 5.3) with GIT and liver model data.

In vitro dosimetry modelling and experimental design report

Report summarizing the exposure regimen for the selected ENM in each in vitro system based on several combined dosimetry models.

PATROLS MODA

Report on the developed MODA for WP6.

Six-monthly E-newsletters documenting updates

Electronic newsletters will be prepared every 6-months for dissemination of PATROLS achievements and updates through a wide contacts distribution list.

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Publications

Using 3D gastrointestinal tract in vitro models with microfold cells and mucus secreting ability to assess the hazard of copper oxide nanomaterials

Author(s): Victor C. Ude, David M. Brown, Vicki Stone, Helinor J. Johnston
Published in: Journal of Nanobiotechnology, Issue 17/1, 2019, ISSN 1477-3155
DOI: 10.1186/s12951-019-0503-1

The importance of inter-individual Kupffer cell variability in the governance of hepatic toxicity in a 3D primary human liver microtissue model

Author(s): Ali Kermanizadeh, David M. Brown, Wolfgang Moritz, Vicki Stone
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-019-43870-8

Real-time cellular impedance monitoring and imaging of biological barriers in a dual-flow membrane bioreactor

Author(s): L. Cacopardo, J. Costa, S. Giusti, L. Buoncompagni, S. Meucci, A. Corti, G. Mattei, A. Ahluwalia
Published in: Biosensors and Bioelectronics, Issue 140, 2019, Page(s) 111340, ISSN 0956-5663
DOI: 10.1016/j.bios.2019.111340

Method for Extraction and Quantification of Metal-Based Nanoparticles in Biological Media: Number-Based Biodistribution and Bioconcentration

Author(s): Fazel Abdolahpur Monikh, Latifeh Chupani, Eliska Zusková, Ruud Peters, Marie Vancová, Martina G. Vijver, Petr Porcal, Willie J.G.M. Peijnenburg
Published in: Environmental Science & Technology, Issue 53/2, 2018, Page(s) 946-953, ISSN 0013-936X
DOI: 10.1021/acs.est.8b03715

Allometric Scaling of physiologically-relevant organoids

Author(s): Chiara Magliaro, Andrea Rinaldo, Arti Ahluwalia
Published in: BioRxiv, Issue Regular online, 2019, ISSN 1476-4687
DOI: 10.1101/559682