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Extracellular vesicles from a natural source for tailor-made nanomaterials

Deliverables

Website and Logo

Ves4Us Logo and Website with intranet section

Optimal culture conditions

Optimised cultivation conditions are determined

Gender Action Plan

The Gender Action Plan will promote equality within the framework of the project

Quality Management system

A quality plan, compatible to UNI EN ISO 9001:2015 and OECD GLP standards, for the management of procedures

Dissemination and Communication Plan

Dissemination and Communication Plan

Training plan

A training plan will describe detailed actions to improve the interdisciplinary methodology and the scientific cross-fertilisation amongst the partners by training staff and students of each participant institution for targeted collaborative experimentation

Functionalization strategy report

Description of the state of the art strategy/ies to be applied for micoalgal EV functionalization

First report on outreach activities

Report on outreach activities

SOPs for microalgal EVs

SOPs for EVs isolation, storage and transport

Characteristics of MiPs

MiPs will be characterised and then tested for selectively capturing EVs from the microalgae cultivation media

Data Management Plan

DMP will be based on the 7-point structure as described in the grant agreement

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Publications

Budding and Fission of Membrane Vesicles: A Mini Review

Author(s): Samo Penič, Luka Mesarec, Miha Fošnarič, Lucyna Mrówczyńska, Henry Hägerstrand, Veronika Kralj-Iglič, Aleš Iglič
Published in: Frontiers in Physics, Issue 8, 2020, ISSN 2296-424X
DOI: 10.3389/fphy.2020.00342

Correlation between adhesion strength and phase behaviour in solid-supported lipid membranes

Author(s): N. Bibissidis, K. Betlem, G. Cordoyiannis, F. Prista-von Bonhorst, J. Goole, J. Raval, M. Daniel, W. Góźdź, A. Iglič, P. Losada-Pérez
Published in: Journal of Molecular Liquids, Issue 320, 2020, Page(s) 114492, ISSN 0167-7322
DOI: 10.1016/j.molliq.2020.114492

A failure mode and effect analysis (FMEA)-based approach for risk assessment of scientific processes in non-regulated research laboratories

Author(s): A. Mascia, A. M. Cirafici, A. Bongiovanni, G. Colotti, G. Lacerra, M. Di Carlo, F. A. Digilio, G. L. Liguori, A. Lanati, A. Kisslinger
Published in: Accreditation and Quality Assurance, 2020, ISSN 0949-1775
DOI: 10.1007/s00769-020-01441-9

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

Author(s): Giulia Di Prima, Fabio Librizzi, Rita Carrotta
Published in: Membranes, Issue 10/9, 2020, Page(s) 222, ISSN 2077-0375
DOI: 10.3390/membranes10090222

Subcellular Localization of uc.8+ as a Prognostic Biomarker in Bladder Cancer Tissue

Author(s): Sara Terreri, Sara Mancinelli, Matteo Ferro, Maria Concetta Vitale, Sisto Perdonà, Luigi Castaldo, Vincenzo Gigantino, Vincenzo Mercadante, Rossella De Cecio, Gabriella Aquino, Marco Montella, Claudia Angelini, Eugenio Del Prete, Marianna Aprile, Angelo Ciaramella, Giovanna L. Liguori, Valerio Costa, George A. Calin, Evelina La Civita, Daniela Terracciano, Ferdinando Febbraio, and Amelia Cimmino
Published in: Cancers, 2021, ISSN 2072-6694
DOI: 10.3390/cancers13040681

Green kiwifruit extracts protect motor neurons from death in a spinal muscular atrophy model in Caenorhabditis elegans

Author(s): Nadia Mazzarella, Ivana Giangrieco, Serena Visone, Pamela Santonicola, Jannis Achenbach, Giuseppina Zampi, Maurizio Tamburrini, Elia Di Schiavi, Maria Antonietta Ciardiello
Published in: Food Science & Nutrition, Issue 7/7, 2019, Page(s) 2327-2335, ISSN 2048-7177
DOI: 10.1002/fsn3.1078

Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Author(s): Božič, Sitar, Junkar, Štukelj, Pajnič, Žagar, Kralj-Iglič, Kogej
Published in: Cells, Issue 8/9, 2019, Page(s) 1046, ISSN 2073-4409
DOI: 10.3390/cells8091046

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author(s): Clotilde Théry, Kenneth W Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon D Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, Tanina Arab, Fabienne Archer, Georgia K Atkin-Smith, D Craig Ayre, Jean-Marie Bach, Daniel Bachurski, Hossein Baharvand, Leonora Balaj, Shawn Baldacchino, Natalie N Bauer, Amy A Baxter, Mary Bebawy, Carla Beckham, Apolonija Bedina Zavec, Abderrahim Benmoussa, Anna
Published in: Journal of Extracellular Vesicles, Issue 7/1, 2018, Page(s) 1535750, ISSN 2001-3078
DOI: 10.1080/20013078.2018.1535750

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Author(s): Carolina Paganini, Umberto Capasso Palmiero, Gabriella Pocsfalvi, Nicolas Touzet, Antonella Bongiovanni, Paolo Arosio
Published in: Biotechnology Journal, 2019, Page(s) 1800528, ISSN 1860-6768
DOI: 10.1002/biot.201800528

Inception Mechanisms of Tunneling Nanotubes

Author(s): Mitja Drab, David Stopar, Veronika Kralj-Iglič, Aleš Iglič
Published in: Cells, Issue 8/6, 2019, Page(s) 626, ISSN 2073-4409
DOI: 10.3390/cells8060626

Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles

Author(s): Christopher Stanly, Maneea Moubarak, Immacolata Fiume, Lilla Turiák, Gabriella Pocsfalvi
Published in: International Journal of Molecular Sciences, Issue 20/24, 2019, Page(s) 6205, ISSN 1422-0067
DOI: 10.3390/ijms20246205

A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly

Author(s): Meiyu Gai, Johanna Simon, Ingo Lieberwirth, Volker Mailänder, Svenja Morsbach, Katharina Landfester
Published in: Polymer Chemistry, 2020, ISSN 1759-9954
DOI: 10.1039/c9py01136f

Vaccination with (1–11)E2 in alum efficiently induces an antibody response to β-amyloid without affecting brain β-amyloid load and microglia activation in 3xTg mice

Author(s): Francesca Mantile, Angelo Capasso, Nadia Villacampa, Maria Donnini, Giovanna L. Liguori, Gabriela Constantin, Piergiuseppe De Berardinis, Michael T. Heneka, Antonella Prisco
Published in: Aging Clinical and Experimental Research, 2019, ISSN 1720-8319
DOI: 10.1007/s40520-019-01414-0

Normal red blood cells’ shape stabilized by membrane’s in-plane ordering

Author(s): L. Mesarec, W. Góźdź, A. Iglič, V. Kralj-Iglič, E. G. Virga, S. Kralj
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-019-56128-0

Role of PA2G4P4 pseudogene in bladder cancer tumorigenesis

Author(s): Laura Pisapia, Sara Terreri, Pasquale Barba, Marianna Mastroianni, Maria Donnini, Vincenzo Mercadante, Alessandro Palmieri, Paolo Verze, Vincenzo Mirone, Vincenzo Altieri, Gianluigi Califano, Giovanna Lucia Liguori, Maria Strazzullo, Amelia Cimmino, Giovanna Del Pozzo
Published in: Biology, Issue 9/4, 2020, Page(s) 66, ISSN 2079-7737
DOI: 10.3390/biology9040066

Minimizing isotropic and deviatoric membrane energy – An unifying formation mechanism of different cellular membrane nanovesicle types

Author(s): Veronika Kralj-Iglič, Gabriella Pocsfalvi, Luka Mesarec, Vid Šuštar, Henry Hägerstrand, Aleš Iglič
Published in: PLOS ONE, Issue 15/12, 2020, Page(s) e0244796, ISSN 1932-6203
DOI: 10.1371/journal.pone.0244796

Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon

Author(s): Zala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič
Published in: International Journal of Nanomedicine, Issue Volume 16, 2021, Page(s) 443-456, ISSN 1178-2013
DOI: 10.2147/ijn.s282200

The effect of lipid phase on liposome stability upon exposure to the mechanical stress

Author(s): Joanna Doskocz, Paulina Dałek, Aleksander Foryś, Barbara Trzebicka, Magdalena Przybyło, Luka Mesarec, Aleš Iglič, Marek Langner
Published in: Biochimica et Biophysica Acta (BBA) - Biomembranes, Issue 1862/9, 2020, Page(s) 183361, ISSN 0005-2736
DOI: 10.1016/j.bbamem.2020.183361

The role of membrane vesiculation and encapsulation in cancer diagnosis and therapy

Author(s): Mitja Drab, Luka Mesarec, Roghayeh Imani, Marko Jeran, Ita Junkar, Veronika Kralj-Iglič, Samo Kralj, Aleš Iglič
Published in: Advances in Biomembranes and Lipid Self-Assembly volume 29, Issue 29, 2019, Page(s) 159-199
DOI: 10.1016/bs.abl.2019.01.007