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Soil bioremediation through flagellated bacteria: unravelling the mechanisms for enhancing bacterial tactic response

Project description

Improving how microorganisms eliminate contaminants in soils

Biological processes can be used to remove contaminants from the environment. This is called bioremediation. Using microorganisms, this process essentially degrades, breaks down and removes contaminants. The EU-funded BIOTAC project will take a novel approach: the macroscale application of the microscale mechanisms underlying the access of self-propelled bacteria to contaminants with limited availability through enhanced dispersal mechanisms. Specifically, the project will focus on the mechanisms operating with flagellated bacteria for bioavailability enhancements. Using carefully selected biological and chemical effectors that favour flagella-mediated taxis and co-mobilisation of immotile degrading strains, the project will develop strategies to improve bacterial transport to pollutant sources. It will also conduct an analysis of the risks and benefits of the new bioremediation technology.

Objective

Bacterial dispersal is a key driver of pollutant turnover in contaminated soils. Flagellated bacteria able to degrade organic pollutants hold tactic responses to a variety of stimuli and reach pollutant hotspots, enhancing the access to poorly bioavailable carbon sources by steepening pollutant concentration gradients at interfaces. However, bacterial motility in porous media is often restricted due to high cell deposition rates and adhesion to soil particles, and in this sense, the modulating role of biological and chemical effectors is decisive. The aim of BIOTAC is the study of the mechanisms operating with flagellated bacteria for bioavailability enhancements and, therefore, a faster turnover of persistent organic pollutants in soils, including polycyclic aromatic hydrocarbons and a pesticide, lindane. We will study, at the microorganism scale, the role of bacterial taxis in chemical gradients (chemotaxis), as well as other tactic responses (repellence, surface taxis) in pollutant bioavailability, modelling and integrating them with other microbial adaptations (i.e. biofilm formation and the production of biosurfactants). We will develop strategies to improve bacterial transport to pollutant sources, using carefully selected biological and chemical effectors (respectively including, mycelial networks and organic compounds -root exudates, humic acids, and fertilizers), that favour flagella-mediated taxis, and co-mobilization of immotile degrading strains. The originality and innovation of the project lies on the macroscale projection of the microscale mechanisms underlying the access of self-propelled bacteria to limitedly available contaminants through an enhanced dispersal. Through experimental and modelling approaches, aiming at balancing biosorption and colloidal transport of the pollutants against biodegradation rates, the project will perform a risk and benefit analysis of the new bioremediation technology.

Fields of science (EuroSciVoc)

Keywords

Programme(s)

Topic(s)

Funding Scheme

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

(opens in new window) H2020-MSCA-IF-2019

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution
€ 160 932,48
Address
CALLE SERRANO 117
28006 Madrid
Spain
Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
Links
Total cost
€ 160 932,48