Project description
The role of macrophages in tissue homeostasis
Macrophages are key immune cells with a scavenging role in different tissues. Emerging evidence indicates that impaired macrophage function in the gut leads to chronic inflammation. The EU-funded DIALOG project is interested to understand the crosstalk between macrophages and their resident tissues and how it dictates macrophage identity. Researchers will characterise the profile of macrophages in different locations in the gut and study the functional outcome of experimentally depleting macrophages. DIALOG will generate important insight into the role of macrophages in maintaining homeostasis in the intestinal niche, which may be shared by other tissues.
Objective
Besides their role as immune sentinels, macrophages fulfil specific functions that are strictly related to their tissue of residence. As an example, the loss of gut macrophages is correlated with impaired crypt cells differentiation which might favour the establishment of chronic intestinal inflammation. Macrophage functions are known to be regulated by tissular niches that imprint the macrophage tissue-specific identity. However, little is known about the “feedback” role of the macrophage for the maintenance and function of its niche. I hypothesise that the loss of a given macrophage subset might imperil its microenvironment through the alteration of its niche cells function or maintenance. Unravelling this feedback mechanism is technically challenging. Current mouse models target macrophages themselves, inducing their rapid replacement by circulating monocytes and thus preventing their long term depletion. Here, I will take advantage of their dependence to a continuous provision of trophic factors by their niche to manipulate and study gut macrophages in a novel manner. First, I will unravel the composition of each gut macrophage niches in a global and unbiased approach by combining single cells and spatial transcriptomics. Next, I will determine the potential cellular sources of trophic factors in each gut niches. Once identified, I will use specific mouse models to delete the trophic factor in the candidate cells to induce a long term depletion of a given macrophage subset. Finally, I will unravel the function of macrophages to their niche by analysing in silico the niche cells transcriptome after macrophage loss to highlight potential molecular interactions between the macrophage and the niche that would induce the survival or differentiation of niche cells. They would eventually be confirmed in vivo by using specific mouse models.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75654 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.