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Friends with benefits – the role of endophytic bacteria in legume nodules containing nitrogen-fixing rhizobia

Project description

Profiling bacterial symbionts in legumes’ root nodules

Plants often form long-term beneficial relationships with bacteria. The symbiosis between legumes and nitrogen-fixing bacteria is among the best studied. The bacteria, called rhizobia, form nodules on the plant root within which the bacteria convert atmospheric nitrogen into ammonia for use by the plant. Rhizobia cohabit with other nodule-associated bacteria, yet the role of these is largely unknown. With the support of the Marie Skłodowska-Curie Actions programme, the FITTER project will investigate the genetic material of all the microbes of legume root nodules. This could enable identification of the genetic features of these bacteria that can be harnessed to increase nutrient availability and plant growth of this important agricultural crop.

Objective

From shoot to root plants are exposed to a diverse range of microorganisms, which can play a major role in plant health, growth and development. A small fraction of bacteria can cause disease, the vast majority of bacteria has no known effect on the host, but there are bacteria that are beneficial to the plant. These microbes are able to protect plants from pathogens, modulate immune responses or assist in nutrient uptake. The best studied beneficial relationship for both plant and bacteria, is the long-term biological interaction (symbiosis) between legumes and nitrogen-fixing bacteria. These bacteria called rhizobia, are hosted in root nodules of legumes such as clover or alfalfa, where they fix atmospheric nitrogen. However, rhizobia are not the sole occupants of nodules and despite early findings of the presence of other nodule associated bacteria (NAB), the diversity and role of NAB in symbiosis and plant growth has remained neglected. Legumes are important crop in agriculture worldwide and as such, the potential of plant growth promoting bacteria and increase of nitrogen-fixation abilities is a major topic of research aiming to enhance agricultural productivity. Investigating the genetic material of all the microbes, the so-called microbiome, of legume root nodules, will allow investigation of genetic features of those bacteria, which contribute to increased plant growth and nutrient availability. Here, I will use a holistic sequencing approach to (i) unravel the diversity and metabolic properties of NAB in legume nodules and (ii) gain insights into the environmental factors affecting NAB community composition, (iii) determine to what extent the bacterial community in the soil (rhizosphere) drives nodule community composition. The proposed research will provide a better understanding of the biological role of all co-occurring bacteria in nodules, in the otherwise binary interaction of legume-rhizobia symbiosis.

Fields of science (EuroSciVoc)

Keywords

Programme(s)

Topic(s)

Funding Scheme

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

(opens in new window) HORIZON-MSCA-2021-PF-01

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Coordinator

UNIVERSITAT WIEN
Net EU contribution
€ 199 440,96
Address
UNIVERSITATSRING 1
1010 Wien
Austria
Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
Links
Total cost
No data