Objective
Cells respond to physical stimuli from their environment, drastically changing their chromatin organization. Direct nuclear mechanotransduction affects cell behavior, but this mechanism is still not fully understood. This unsolved link is due to the difficulty of finding tools to generate precise mechanical signals and observe changes in chromatin at the nanoscale level.
NanoCHROMS aims to understand the effect of nuclear mechanotransduction on nuclear architecture. We aim to characterize the mechanism driving localized chromatin structure changes in response to direct physical stimuli, and how these changes relate to cellular state. NanoCHROMS will exploit two novel and powerful technologies, Nanofabrication and Single Molecule Localization Microscopy (SMLM). For that, we will use nanopillars as nanomechanical actuators. By culturing somatic, stem and cancer cells in a fabricated nanopillar surface, purposely designed to be SMLM-compatible, we can exert a precise, controlled, and replicable force in the nucleus.
Using SMLM, we can unveil higher order chromatin folding with nanometric precision, obtaining positional information of DNA and nucleosome packaging, directly related to cell function. By using SMLM in cells growing on nanopillared surfaces, we can establish the changes of chromatin in direct relation to the physical point of application of the stimulus at the nanoscale. Finally, using sequence-specific imaging approaches (Oligopaints) we will study the nuclear positioning and compaction of genes whose expression is drastically affected by mechanotransduction.
NanoCHROMS will contribute to understanding the mechanisms driving expression changes, maintenance of stemness and oncogenesis by mechanostimulus, being a steppingstone in projects working in the interface between cell biology and nanomaterials science. Finally, NanoCHROMS will develop a systematic and quantitative method to evaluate the effect of different nanostructures on nuclear architecture.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences cell biology
- natural sciences physical sciences optics microscopy
- medical and health sciences clinical medicine oncology
- engineering and technology nanotechnology nano-materials
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
28049 Madrid
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.