Obiettivo 1) To study the metabolism of inorganic arsenic in a population in Argentina heavily exposed to As in drinking water.2) To evaluate the mechanism(s) by which As interferes with DNA repair.3) To evaluate the influence of As on the yield of sister chromatid exchanges and chromosomal aberrations in human lymphocytes exposed to chemical mutagens or UV-B.A group of individuals exposed to inorganic arsenic in drinking water from the Salta province will be investigated to evaluate its metabolism in human. Blood, plasma, urine, nail and hair follicles will be collected and the content of As and its metabolites will be estimated. Blood samples from these individuals will be processed for cytogenetic investigations.The basis for the potentiating effects of inorganic arsenic on the mutagenic effects of chemicals/UV will be elucidated by carrying out DNA repair studies as well as cytogenetic studies. In DNA repair studies, cell lines known to be specifically deficient in certain repair steps will be used. In addition, an in vitro repair assay on plasmids carrying specific lesions in their DNA and using cell extracts in the presence or absence of As, or cell extracts from As treated cells will be used.The influence of As on the yield of chromosomal aberrations and SCEs in human lymphocytes or fibroblasts induced by diverse chemicals with different modes of action or UV-B will be investigated. Chromosome painting using FISH technique will be used to detect translocations in these experiments. Campo scientifico engineering and technologyenvironmental engineeringwater treatment processesdrinking water treatment processeshumanitiesartsvisual artsnatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencesinorganic chemistrymetalloidsnatural sciencesbiological sciencesgeneticschromosomes Programma(i) FP3-ENV 1C - Specific research and technological development programme (EEC) in the field of the environment, 1990-1994 Argomento(i) 040203 - Environment and human health Invito a presentare proposte Data not available Meccanismo di finanziamento CSC - Cost-sharing contracts Coordinatore Rijksuniversiteit Leiden Contributo UE Nessun dato Indirizzo 72,Wassenaarseweg 2300 RA Leiden Paesi Bassi Mostra sulla mappa Costo totale Nessun dato Partecipanti (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto KAROLINSKA INSTITUTE Svezia Contributo UE Nessun dato Indirizzo Nobels Vaeg, 13 171 77 STOCKHOLM Mostra sulla mappa Costo totale Nessun dato Universität Bremen Germania Contributo UE Nessun dato Indirizzo Bibliothekstraße 1 28334 Bremen Mostra sulla mappa Costo totale Nessun dato