STUDIES OF PROLIFERATIVE KIDNEY DISEASE WITH PARTICULAR REFERENCE TO PROPHYLAXIS AND GEOGRAPHICAL DISTRIBUTION IN EUROPE

Projektinformationen

ID Finanzhilfevereinbarung: AIR21570

Projekt abgeschlossen

Startdatum 1 März 1994

Enddatum 28 Februar 1997

Finanziert unter

• Specific research and technological development and demonstration programme (EEC) in the field of agriculture and agro-industry, including fisheries, 1990-1994

Gesamtkosten € 757 204,00

EU-Beitrag € 719 731,00

Koordiniert durch

UNIVERSITY OF STIRLING
United Kingdom

Ziel

The object of the study is to use specific probes to identify the surface antigens and life cycle stages of PK"X", the causative agent of PKD, so that an effective vaccine against the disease can be developed. In addition, the distribution and level of the disease in Europe will be established. Monoclonal antibody and lectin probes will be used to identify and quantify the parasite in fish tissues and water samples collected during the PKD season throughout the course of the project. Monoclonal antibody probes, developed during the "FAR" project, will be used to purify PK"X" cell antigen from host cells using affinity chromatography. These will be used to inject mice and produce a further battery of monoclonal antibodies to different surface antigens. Such probes will be used in life-cycle studies and enable identification of life-cycle stages prior to those currently identified using histological methods. The detailed immune response of fish following natural PKD infection will be investigated. ELISA and dot-filter tests will also be used to detect specific PK"X" cells from water samples by linking monoclonal antibodies to filters. Such material will then be used in attempts at culture of the PK"X" cells in vitro. A series of passive immunisation experiments will be carried out to establish the nature of the protective antigens. This information will then be used to formulate and field test potential vaccines.

Wissenschaftliches Gebiet

• medical and health sciences › basic medicine › immunology › immunisation
• medical and health sciences › basic medicine › pharmacology and pharmacy › pharmaceutical drugs › vaccines
• medical and health sciences › clinical medicine › nephrology › kidney diseases

Programm/Programme

• FP3-AIR - Specific research and technological development and demonstration programme (EEC) in the field of agriculture and agro-industry, including fisheries, 1990-1994

Thema/Themen

• 251 - Health of aquacultural stocks

Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

CSC - Cost-sharing contracts

Koordinator

UNIVERSITY OF STIRLING

• EU-Beitrag: Keine Daten
• Adresse: FK9 4LA STIRLING ; Vereinigtes Königreich
• Gesamtkosten: Keine Daten

Beteiligte (4)

Institut National de la Recherche Agronomique (INRA)

Frankreich

• EU-Beitrag: Keine Daten
• Adresse: Domaine de Vilvert ; 78352 Jouy-en-Josas
• Gesamtkosten: Keine Daten

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta

Italien

• EU-Beitrag: Keine Daten
• Adresse: Via Bologna 148 ; 10154 Torino
• Gesamtkosten: Keine Daten

SANOFI SANTE NUTRITION ANIMALE

Frankreich

• EU-Beitrag: Keine Daten
• Adresse: 126 ZONE INDUSTRIELLE DE LA BALLASTIERE ; 33501 LIBOURNE
• Gesamtkosten: Keine Daten

UNIVERSITY COLLEGE CORK

Irland

• EU-Beitrag: Keine Daten
• Adresse: 30 CORK
• Gesamtkosten: Keine Daten