Objective All stem cell populations are controlled by a number of intrinsic and external regulatory mechanisms that function in maintaining balance between stem cell self-renewal and commitment to differentiation.Recent evidence also suggests that some cancers are controlled by a small population of tumour cells that exhibit stem cell like qualities (referred to as cancer stem cells) lending to the hypothesis that cancer is a disease of uncontrolled stem cell self-renewal. This proposal aims to elucidate how the transcription factors c-Myc, N-Myc and c-Jun interact with each other to control haematopoietic stem cell (HSC) fate.We will induce the conditional deletion and over-expression of these genes in mouse adult HSCs and single/double mutant HSCs will be subsequently injected into lethally irradiated mice, quantified by limited dilution assays and the effects on the stem and progenitor cell pool and each of the haematopoietic lineages assessed by cell cycle analysis and FACS.Particular focus will be placed on identifying how these proteins govern the molecular interaction between HSCs and their micro-environment (stem cell niche). An in depth knowledge of these mechanisms will be crucial in the development of conditions suitable for the in vitro expansion of adult HSC populations for clinical applications.This project also aims to elucidate the role of the c-Myconcogene in an AP-1 driven chronic myeloid leukaemia (CML) model. We will over-express the c-Myconcogene in a JunB-derived CML mouse model to address the r ole of c-Myc in regulating the malignant expansion of leukaemic stem cells.These data will provide insight into how signalling pathways governing normal stem cell homeostasis regulate tumour formation, thus allowing the development of novel treatments for the elimination of cancer cells. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicineoncologyleukemiamedical and health sciencesclinical medicineembryologymedical and health sciencesbasic medicinephysiologyhomeostasis Keywords origin of cancer stem cells stem cell niche interactions stem cell self-renewal Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Topic(s) MOBILITY-2.3 - Marie Curie Incoming International Fellowships (IIF) Call for proposal FP6-2005-MOBILITY-7 See other projects for this call Funding Scheme IIF - Marie Curie actions-Incoming International Fellowships Coordinator ECOLE POLYTECHNIQUE FÉDÉRALE DE LAUSANNE EU contribution No data Address Bâtiment CE-3.316, Station 1 EPALINGES Switzerland See on map Links Website Opens in new window Total cost No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all SWISS INSTITUTE FOR EXPERIMENTAL CANCER RESEARCH Switzerland EU contribution No data Address Ch. des Boveresses 155 EPALINGES See on map Links Website Opens in new window Total cost No data
