Objective
Eukaryotic cells contain myriad signaling pathways used to transmit information from the plasma membrane to the nucleus. Given the large number of pathways in a cell, how is it that a given stimulus leads to a precise response? This problem is particularly acute in kinase pathways, such as mitogen-activated protein kinase (MAPK) cascades, which often share common protein components. Over the last decade, however, a paradigm that has emerged is that signaling pathway specificity can be mediated, in part, by scaffolding proteins that bind pathway members and restrict them to act only on one another. In yeast, as well as in mammals, several scaffold proteins for MAP-kinase cascades have been identified. These scaffolds allow one or more common kinases to function in distinct signaling pathways without improper cross-talk. However, very little is known about the mechanism by which these scaffold molecules physically control transmission of signaling information. We therefore plan to determine the three-dimensional structures of MAP-kinase modules of the yeast mating and high osmolarity response pathways, which are organized by two non-related scaffold proteins: Ste5 and Pbs2. The components (four kinases and two scaffold proteins) will be expressed as recombinant proteins and the complex reconstituted in vitro. We will use a multifaceted approach with the following specific aims: (1) to determine the structure of the reconstituted Ste5 and Pbs2 scaffolded complex by X-ray crystallography or electron cryomicroscopy and (2) to identify well-ordered fragments of the scaffolds that interact with individual member kinases to determine the structure of informative sub-complexes by X-ray crystallography.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences biological sciences zoology mammalogy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-3-IRG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1053 Budapest
Hungary
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.