Objective
DNA damage response (DDR) monitors chromosome status to ensure correct homologous recombination, genomic integrity and chromosome segregation. Thus the effect of DDR activation upon exogenous DNA breaks has been intensely studied. Less is known about the effects of such mechanisms due to endogenous breaks. In multicellular organisms meiosis is a classical example of double strand break (DSB) production and repair occurring concomitantly with gamete development. In Drosophila persistent meiotic DSBs activates the ATR/Mei-41 checkpoint, delays progression through meiosis and causes defects in DNA condensation in the oocyte nucleus. Checkpoint activation has also been linked to decreased levels of the TGFα-like molecule Gurken (GRK), which controls normal eggshell patterning. I used this easy scorable eggshell phenotype in a germ line mosaic screen to identify new genes affecting meiotic progression, DNA condensation and GRK signalling. My work on these mutants challenged the hypothesis that checkpoint activation upon persistent DSBs is exclusively mediated by ATR kinase and instead reveals a more complex network of interactions that link DSB formation, checkpoint activation, meiotic delay, DNA condensation and GRK synthesis. To understand how multicellular organisms monitor chromatin integrity during meiosis and how these surveillance mechanisms affect development I propose the study of the cohesion gene dPds5 in early and late meiosis, the characterization of two rasiRNA genes montecristo and sancho-panza in transposon silencing during oogenesis and the identification of genes downstream of checkpoint pathway mediating eggshell polarity response upon checkpoint activation. I discuss two major new ideas explored in this project: 1. cohesion is required for eggshell polarity, and 2. various branches of the DDR monitor aspects of chromatin independently of DSB.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics heredity
- natural sciences biological sciences genetics chromosomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IRG-2008
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1067-001 LISBOA
Portugal
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.