Obiettivo Dopamine receptors are members of G-protein coupled receptor superfamily. These receptors are the key point of dopaminergic system, which controls the regulation of memory, attention, food intake, endocrine regulation, psychomotor activity and positive reinforcement. To regulate so many critically important neurological events, dopamine receptors have complex interactions with other receptors and ion channels. In this study, a trimeric complex comprising D2 receptor -which is a subtype of dopamine receptors- , A2A adenosine receptor being another G-protein coupled receptor and a ligand gated ion channel N-methyl-D-aspartate (NMDA) receptor will be investigated. The aim of this project will be the application of a novel technique we refer as SplitGFP-FRET for the first time. For this study, “Fluorescence Resonance Energy Transfer” and “Split GFP” methods will be combined so as to reveal the trimeric D2 dopamine receptor- A2A adenosine receptor-NMDA receptor interactions. With the proposed study, a fluorescence technique to analyze live cell culture will be developed. This model will potentially lead to understanding of molecular mechanisms of many neuropathological conditions like schizophrenia and Parkinson’s disease because these disorders have already been shown to be associated with dysregulations in dopaminergic systems. Therefore, this project aims to reveal detailed links between schizophrenia and interactions of D2 dopamine receptor with other receptors and ion channels. The developed live cell model will also be useful for testing anti-psychotic drugs whether they have effects on disruption of protein-protein interactions, which will, in turn, ease screening of newly designed drugs. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsmedical and health sciencesbasic medicineneurologyparkinsonmedical and health sciencesclinical medicinepsychiatryschizophrenia Programma(i) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants" Invito a presentare proposte FP7-PEOPLE-2010-RG Vedi altri progetti per questo bando Meccanismo di finanziamento MC-IRG - International Re-integration Grants (IRG) Coordinatore MIDDLE EAST TECHNICAL UNIVERSITY Contributo UE € 100 000,00 Indirizzo DUMLUPINAR BULVARI 1 06800 Ankara Turchia Mostra sulla mappa Regione Batı Anadolu Ankara Ankara Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo İrem Dikmen Toker (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
