Objective
"Bacterial infection into host cells is an important and highly active field of research. Understanding the interactions and the distribution of the host-cell scaffolding protein network during bacterial entry poses a major challenge. The molecular architecture of actin comet tails, filamentous structures assembled by internalized bacteria to move inside the host-cell cytoplasm and from cell-to-cell, remains unknown. Cryo-electron tomography (cryo-ET) is the most advanced method for visualizing the architecture of hydrated cells at a resolution better than 5 nm. Cryo-ET will be used to visualize the three dimensional (3D) cytoskeleton reorganization directly in eukaryotic cells infected by Listeria. Measurements will be performed at cryo-temperatures, on vitrified cell samples preserved in a close-to-life state. Specimen thickness limitations will be overcome by the use of the focused ion beam (FIB) micro-machining method, to obtain 500 nm thick samples as required for the collection of good data. Cryo-ET will be combined with correlative cryo-fluorescence microscopy, to localize the scaffolding components recruited during Listeria uptake and motility: host-cell actin, septin and clathrin. We expect to achieve nanometer resolution maps of the cell area of interest. The distribution and the ultrastructure of the cytoskeletal scaffold at Listeria entry and of Listeria actin comet tails will be provided. The work will provide unprecedented insight into cytoskeleton architecture during bacterial pathogenesis. The applicant obtained her PhD at a structural biology institute in France. Joining the Baumeister laboratory in Germany, and collaborating with the Cossart group, will allow her to address new challenging questions on the structural organisation of the cell, at unprecedented resolution. The acquired combination of skills at a world-class level will contribute significantly to her professional maturity, and to increase the competitiveness of European science."
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences physical sciences astronomy planetary sciences comets
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.