Project description
Unlocking endothelial cell biology
Existing therapies against cardiovascular disease often aim to stimulate functional angiogenesis through the delivery of vascular growth factors. This is considered to be beneficial for angiogenesis as it increases endothelial cell proliferation and sprouting. However, highly mitogenic environments also cause a significant cell-cycle arrest of endothelial cells. The EU-funded AngioUnrestUHD project will use advanced genetic methods and single-cell profiling to modulate and elucidate the mechanisms responsible for this arrest. The therapeutic targeting of these mechanisms may enable a more effective induction of angiogenesis in cardiovascular disease or its inhibition in cancer or other vascular disorders.
Objective
Therapeutic modulation of vascular cell proliferation and migration is essential for the effective inhibition of angiogenesis in cancer or its induction in cardiovascular disease. The current view is that an increase in growth factor levels or mitogenic stimulation is beneficial for angiogenesis, since it leads to an increase in both endothelial proliferation and sprouting.
Through the use of innovative genetic and imaging approaches, we have recently elucidated a previously unappreciated, context-dependent mechanism whereby highly mitogenic environments can be detrimental for angiogenesis and lead to the cell-cycle arrest of endothelial cells (ECs), which ultimately impairs vascular growth.
The identified mechanism may explain the failed or inefficient promotion of functional angiogenesis by vascular growth factor delivery therapies, such as those used to treat ischemic cardiovascular disease. We propose that a better understanding and modulation of the identified hypermitogenic arrest process may allow angiogenesis to be induced more effectively.
Taking advantage of recent advances in DNA synthesis, CRISPR gene editing, microscopy and single-cell profiling technologies, we have developed new genetic tools, animal models and methods of broad relevance that enable the study of gene function with higher reliability, throughput and definition.
We propose to use these novel research tools and methods to significantly increase understanding of the biology of blood vessels in distinct physiological and pathological contexts.
We will then use this new knowledge to identify better strategies to promote vascular development in ischemic cardiovascular disease, heal vascular malformations, or inhibit angiogenesis in tumours.
Fields of science
- medical and health sciencesmedical biotechnologygenetic engineeringgene therapy
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesphysical sciencesopticsmicroscopy
- medical and health sciencesclinical medicinecardiologycardiovascular diseases
- medical and health sciencesclinical medicineoncology
Programme(s)
Funding Scheme
ERC-COG - Consolidator GrantHost institution
28029 Madrid
Spain