Descripción del proyecto
Dilucidación de la biología de las células endoteliales
Las terapias existentes contra las enfermedades cardiovasculares suelen centrarse en estimular la angiogénesis funcional a través de la administración de factores de crecimiento vascular. Estos se consideran beneficiosos para la angiogénesis puesto que incrementan la proliferación y la gemación de las células endoteliales. Sin embargo, los entornos altamente mitogénicos también provocan una importante interrupción del ciclo de las células endoteliales. El proyecto AngioUnrestUHD, financiado con fondos europeos, utilizará métodos genéticos avanzados y el perfilado de células individuales para modular y dilucidar los mecanismos responsables de esta interrupción. La terapia centrada en estos mecanismos podría facilitar una inducción más eficaz de la angiogénesis en las enfermedades cardiovasculares o su inhibición en el cáncer u otros trastornos vasculares.
Objetivo
Therapeutic modulation of vascular cell proliferation and migration is essential for the effective inhibition of angiogenesis in cancer or its induction in cardiovascular disease. The current view is that an increase in growth factor levels or mitogenic stimulation is beneficial for angiogenesis, since it leads to an increase in both endothelial proliferation and sprouting.
Through the use of innovative genetic and imaging approaches, we have recently elucidated a previously unappreciated, context-dependent mechanism whereby highly mitogenic environments can be detrimental for angiogenesis and lead to the cell-cycle arrest of endothelial cells (ECs), which ultimately impairs vascular growth.
The identified mechanism may explain the failed or inefficient promotion of functional angiogenesis by vascular growth factor delivery therapies, such as those used to treat ischemic cardiovascular disease. We propose that a better understanding and modulation of the identified hypermitogenic arrest process may allow angiogenesis to be induced more effectively.
Taking advantage of recent advances in DNA synthesis, CRISPR gene editing, microscopy and single-cell profiling technologies, we have developed new genetic tools, animal models and methods of broad relevance that enable the study of gene function with higher reliability, throughput and definition.
We propose to use these novel research tools and methods to significantly increase understanding of the biology of blood vessels in distinct physiological and pathological contexts.
We will then use this new knowledge to identify better strategies to promote vascular development in ischemic cardiovascular disease, heal vascular malformations, or inhibit angiogenesis in tumours.
Ámbito científico
- medical and health sciencesmedical biotechnologygenetic engineeringgene therapy
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesphysical sciencesopticsmicroscopy
- medical and health sciencesclinical medicinecardiologycardiovascular diseases
- medical and health sciencesclinical medicineoncology
Programa(s)
Régimen de financiación
ERC-COG - Consolidator GrantInstitución de acogida
28029 Madrid
España