Description du projet
Un microscope innovant capable de prélever des cellules
REVEAL est un projet pluridisciplinaire financé par l’UE, qui entend développer une technique de microscopie révolutionnaire, dotée de capacités d’imagerie des cellules vivantes en 2D et en 3D. À l’aide de l’apprentissage automatique, le microscope sera capable de percevoir et d’interpréter des événements cellulaires susceptibles de déboucher sur une transformation maligne. Cela fournira aux utilisateurs l’opportunité de détecter et de collecter différents types de cellules du foie, qui seront par la suite analysées aux niveaux moléculaire et biochimique. Le projet associera les phénotypes cellulaires observés à travers le microscope à des profils d’expression génétique, fournissant ainsi des connaissances importantes sur l’origine cellulaire du cancer du foie.
Objectif
The REVEAL project aims at installing a novel microscope at the interface of instrumentation, machine learning and Biology. This new microscopy technique will rely heavily on neural networks to be able to perceive, interpret, conjecture, infer, anticipate and act. The present project intends to lay down the foundations of this ‘neuronal microscopy’ in the realms of 2D and 3D cell live imaging.
To achieve this goal, we will develop the necessary tools and the first instruments that will demonstrate the feasibility and the power of this new microscopy framework. In particular, we will develop a ‘cell picking neuronal microscope’ that will allow us to detect and collect different subtype of liver cells that are due to become tumoral and analyse them ahead of tumour manifestation. This analysis will help us to characterize the cellular heterogeneity known to exist at the origin of liver cancer. It will provide a direct mapping between cellular phenotypes observed under microscope and gene expression present in the early stages of liver cancer. Overall, we are thus defining a novel methodology, giving a central place to neuronal microscopy in the understanding of the cellular origin of the diseases.
REVEAL is a multidisciplinary project engaging 7 EU partners (2 being SMEs) from 4 countries covering the RTO, academic and industrial worlds, including medical doctors. An implementation plan is presented in the form of 6 work packages, 4 of them being technical. Synergy in communication and dissemination by the several partners and stakeholders will permit to maximize the project impact. Solutions to overcome the technological barriers, as well as appropriate deliverables, tasks, milestones and risks are presented, in order to complete the project objectives in due time . The REVEAL project outcomes are fully in line with the H2020 ICT-36-2020 call, subtopic iv, as it will develop novel methods and devices as research tools to understand the cellular origin of diseases.
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RIA - Research and Innovation actionCoordinateur
75015 PARIS 15
France