Compact Cell-Imaging Device to provide insight into the cellular origins of diseases and to aid in the development of novel therapeutics

Project Information

CoCID

Grant agreement ID: 101017116

DOI 10.3030/101017116

EC signature date 4 December 2020

Start date 1 January 2021

End date 31 December 2024

Funded under

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT)

Total cost € 5 686 215,00

EU contribution € 5 686 215,00

Coordinated by

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Ireland

Deliverables

Open Research Data Pilot (6)

Imaging of HEV-infected ex vivo porcine precision cut liver slices
Structural differences in HEV-infected pig and human cell lines
Correlation of the kinetics normal cell homeostasis of viral antigen and genomic RNA with restoration of

Correlation of the kinetics of viral antigen and genomic RNA with restoration of normal cell homeostasis

3D model of infected lung epithelial cell
Calibration table of x-ray absorption coefficient for adherent cells.

The table will provide calibration value to translated x-ray linear absorption coefficient obtained with lab-based SXM with respect to the synchrotron source for EM grid sample holders and limited angle tomography.

Calibration table of x-ray absorption coefficient for suspended cells.

The table will provide calibration value to translated x-ray linear absorption coefficient obtained with lab-based SXM with respect to the synchrotron source for cells in suspension and full rotation tomography.

Demonstrators, pilots, prototypes (1)

Fully tested in-line FM

Fully validated FM as indicated through bench tests and inline imaging of fluorescent nanospheres

Publications

Peer reviewed articles (8)

Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

Author(s): Victoria Castro, Ana Joaquina Pérez-Berna, Gema Calvo, Eva Pereiro*, and Pablo Gastaminza
Published in: ACS Nano, Issue 2023, 17, 22, 2023, Page(s) 22708–22721, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.3c07265

Monitoring reversion of hepatitis C virus-induced cellular alterations by direct-acting antivirals using cryo soft X-ray tomography and infrared microscopy

Author(s): Ana J. Pérez-Berná; Nuria Benseny-Cases; María José Cubillas Rodríguez; Ricardo Valcárcel; José L. Carrascosa; Pablo Gastaminza; Eva Pereiro
Published in: Acta Crystallographica Section D: Structural Biology, Issue 1, 2021, ISSN 2059-7983
Publisher: IUCr
DOI: 10.1107/s2059798321009955

Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane

Author(s): Vesa Aho ,Sami Salminen, Salla Mattola, Alka Gupta, Felix Flomm, Beate Sodeik, Jens B. Bosse, Maija Vihinen-Ranta
Published in: PLOS PATHOGENS, 2021, ISSN 1553-7366
Publisher: Public Library of Science
DOI: 10.1371/journal.ppat.1010132

Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells

Author(s): Jian-Hua Chen,Bieke Vanslembrouck, Axel Ekman, Vesa Aho, Carolyn A. Larabell, Mark A. Le Gros, Maija Vihinen-Ranta, Venera Weinhardt
Published in: Viruses, 2022, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14122651

Compact Cell Imaging Device (CoCID) to provide insights into the cellular origins of viral infections

Author(s): Kenneth Fahy, Venera Weinhardt, Maija Vihinen-Ranta, Nicola Fletcher, Dunja Skoko, Eva Pereiro, Pablo Gastaminza, Ralf Bartenschlager, Dimitri Scholz, Axel Ekman, Tony McEnroe
Published in: Journal of Physics: Photonics, 2021, ISSN 2515-7647
Publisher: IOP Publishing
DOI: 10.1088/2515-7647/abfc5a

A protocol for full-rotation soft X-ray tomography of single cells

Author(s): Jian-Hua Chen, Bieke Vanslembrouck, Valentina Loconte, Axel Ekman, Mirko Cortese, Ralf Bartenschlager, Gerry McDermott, Carolyn A. Larabell, Mark A. Le Gros, and Venera Weinhardt
Published in: STAR Protocols, 2022, ISSN 2666-1667
Publisher: Elsevier
DOI: 10.1016/j.xpro.2022.101176

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.

Author(s): Valentina Loconte; Valentina Loconte; Jian-Hua Chen; Jian-Hua Chen; Mirko Cortese; Axel Ekman; Mark A. Le Gros; Mark A. Le Gros; Carolyn A. Larabell; Carolyn A. Larabell; Ralf Bartenschlager; Ralf Bartenschlager; Venera Weinhardt; Venera Weinhardt
Published in: Cell Reports Methods, Issue 2, 2021, ISSN 2667-2375
Publisher: Cell Press
DOI: 10.1016/j.crmeth.2021.100117

Imaging of Virus-Infected Cells with Soft X-ray Tomography

Author(s): Damià Garriga, Francisco Javier Chichón, Bárbara M. Calisto, Diego S. Ferrero, Pablo Gastaminza, Eva Pereiro and Ana Joaquina Pérez-Berna
Published in: Viruses, Issue Monthly, 2021, ISSN 1999-4915
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13112109