Compact Cell-Imaging Device to provide insight into the cellular origins of diseases and to aid in the development of novel therapeutics

Livrables

Imaging of HEV-infected ex vivo porcine precision cut liver slices
Structural differences in HEV-infected pig and human cell lines
Correlation of the kinetics normal cell homeostasis of viral antigen and genomic RNA with restoration of

Correlation of the kinetics of viral antigen and genomic RNA with restoration of normal cell homeostasis

3D model of infected lung epithelial cell
Calibration table of x-ray absorption coefficient for adherent cells.

The table will provide calibration value to translated x-ray linear absorption coefficient obtained with lab-based SXM with respect to the synchrotron source for EM grid sample holders and limited angle tomography.

Calibration table of x-ray absorption coefficient for suspended cells.

The table will provide calibration value to translated x-ray linear absorption coefficient obtained with lab-based SXM with respect to the synchrotron source for cells in suspension and full rotation tomography.

Fully tested in-line FM

Fully validated FM as indicated through bench tests and inline imaging of fluorescent nanospheres

Publications

Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

Auteurs: Victoria Castro, Ana Joaquina Pérez-Berna, Gema Calvo, Eva Pereiro*, and Pablo Gastaminza
Publié dans: ACS Nano, Numéro 2023, 17, 22, 2023, Page(s) 22708–22721, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.3c07265

Monitoring reversion of hepatitis C virus-induced cellular alterations by direct-acting antivirals using cryo soft X-ray tomography and infrared microscopy

Auteurs: Ana J. Pérez-Berná; Nuria Benseny-Cases; María José Cubillas Rodríguez; Ricardo Valcárcel; José L. Carrascosa; Pablo Gastaminza; Eva Pereiro
Publié dans: Acta Crystallographica Section D: Structural Biology, Numéro 1, 2021, ISSN 2059-7983
Éditeur: IUCr
DOI: 10.1107/s2059798321009955

Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane

Auteurs: Vesa Aho ,Sami Salminen, Salla Mattola, Alka Gupta, Felix Flomm, Beate Sodeik, Jens B. Bosse, Maija Vihinen-Ranta
Publié dans: PLOS PATHOGENS, 2021, ISSN 1553-7366
Éditeur: Public Library of Science
DOI: 10.1371/journal.ppat.1010132

Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells

Auteurs: Jian-Hua Chen,Bieke Vanslembrouck, Axel Ekman, Vesa Aho, Carolyn A. Larabell, Mark A. Le Gros, Maija Vihinen-Ranta, Venera Weinhardt
Publié dans: Viruses, 2022, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14122651

Compact Cell Imaging Device (CoCID) to provide insights into the cellular origins of viral infections

Auteurs: Kenneth Fahy, Venera Weinhardt, Maija Vihinen-Ranta, Nicola Fletcher, Dunja Skoko, Eva Pereiro, Pablo Gastaminza, Ralf Bartenschlager, Dimitri Scholz, Axel Ekman, Tony McEnroe
Publié dans: Journal of Physics: Photonics, 2021, ISSN 2515-7647
Éditeur: IOP Publishing
DOI: 10.1088/2515-7647/abfc5a

A protocol for full-rotation soft X-ray tomography of single cells

Auteurs: Jian-Hua Chen, Bieke Vanslembrouck, Valentina Loconte, Axel Ekman, Mirko Cortese, Ralf Bartenschlager, Gerry McDermott, Carolyn A. Larabell, Mark A. Le Gros, and Venera Weinhardt
Publié dans: STAR Protocols, 2022, ISSN 2666-1667
Éditeur: Elsevier
DOI: 10.1016/j.xpro.2022.101176

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.

Auteurs: Valentina Loconte; Valentina Loconte; Jian-Hua Chen; Jian-Hua Chen; Mirko Cortese; Axel Ekman; Mark A. Le Gros; Mark A. Le Gros; Carolyn A. Larabell; Carolyn A. Larabell; Ralf Bartenschlager; Ralf Bartenschlager; Venera Weinhardt; Venera Weinhardt
Publié dans: Cell Reports Methods, Numéro 2, 2021, ISSN 2667-2375
Éditeur: Cell Press
DOI: 10.1016/j.crmeth.2021.100117

Imaging of Virus-Infected Cells with Soft X-ray Tomography

Auteurs: Damià Garriga, Francisco Javier Chichón, Bárbara M. Calisto, Diego S. Ferrero, Pablo Gastaminza, Eva Pereiro and Ana Joaquina Pérez-Berna
Publié dans: Viruses, Numéro Monthly, 2021, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13112109

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