Project description
Dissecting the process of oral virus infection
Insects serve as vectors for the transmission of many viral diseases of humans and crops. Transmission involves the oral acquisition of viruses by insects, suggesting that disrupting this route may serve as an intervention strategy. The EU-funded DmGAR project aims to study viral infection processes in the insect gut. Researchers will employ Drosophila melanogaster as a model and investigate the impact of viruses on the epithelial cells of the gut. More specifically, they will characterise the route and kinetics of viruses and investigate host immune responses in the gut. Results will improve our current knowledge on viral infections and pave the way for novel strategies to counteract them.
Objective
Insect-mediated transmission of viruses is an increasing threat to public health and agricultural productivity in Europe and abroad. New strategies to disrupt transmission of viruses by insects are essential and will be facilitated by knowledge of infection processes and antiviral mechanisms. Most transmission cycles involve oral acquisition of viruses by insects, but knowledge of oral virus infections is limited. Here I propose the DmGAR project (Drosophila melanogaster gut antiviral responses) to provide the first detailed picture of oral virus infection processes in the insect gut. I aim to harness the experimental tractability of D. melanogaster to characterize the dynamics of and genetic responses to oral virus infection and to investigate the interface of viral infection with intestinal physiology.
I hypothesize that stem cell-driven gut epithelium renewal is coordinated with immune mechanisms and is crucial for clearance of oral virus infections. To investigate this hypothesis and accomplish the objectives of DmGAR, I will bring my expertise in host-virus interactions to the Saleh laboratory to study oral infections with naturally infecting viruses of D. melanogaster. I will spatially and temporally define the kinetics and outcomes of infection, determine whether the viruses spread beyond the gut, and if escape from the gut influences infection outcome. Informed by these data, I will evaluate if virus infection impacts rates of epithelial cell death and/or proliferation in the gut and if blockage of epithelial cell turnover influences virus titer, infection outcome, or infection kinetics. I will complement these studies by profiling cell-type-specific gene expression and assaying activation of cell signaling pathways to develop a mechanistic model of the response to oral virus infection in the insect gut. Through these approaches, DmGAR will provide robust and biologically relevant data that will enhance understanding of natural virus infection processes
Fields of science
- medical and health scienceshealth sciencespublic health
- natural sciencesbiological sciencesmicrobiologyvirology
- medical and health sciencesbasic medicinephysiology
- natural sciencesbiological scienceszoologyentomology
- medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantivirals
Keywords
Programme(s)
Funding Scheme
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinator
75724 Paris
France