Handling of iron in nitrogen-fixing endosymbiotic bacteria
Rhizobia-legume symbiosis is the main route for sustainable nitrogen to contribute to soil fertility. During symbiosis, legumes develop nodules hosting endosymbiotic bacteria expressing nitrogenase, a unique iron and molibdenum metalloenzyme that transforms atmospheric nitrogen into ammonia that is utilised by plants. The EU-funded IronFeRhizo project will combine biochemical and molecular biology techniques to determine how much iron is required by nitrogen-fixing bacteria, which genes are required in its transport into the cell, and how much is used to metallate the nitrogenase and other metalloproteins. The techniques developed will facilitate research into mineral nutrition in other soil bacteria, how cells partition an element among different metalloproteins, and how iron homeostasis can help us optimise nitrogen fixation in rhizobia.
Fields of science
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesphysical sciencesopticsspectroscopyabsorption spectroscopy
- natural sciencesbiological sciencesbiological behavioural sciencesethologybiological interactions
- medical and health sciencesbasic medicinephysiologyhomeostasis
- natural sciencesbiological sciencesmolecular biology