Project description
Study of the cellular entry mechanisms for coronaviruses with different pathogenicity
Coronaviruses (CoVs) have created several outbreaks, including the current global pandemic. Some viruses of this family cause only mild conditions, and the reasons for variations in the severity of the diseases are poorly understood. CoVs attach to the cells via a specific interaction between the viral spike glycoprotein (CoV-S) and receptors on the cell surface. The EU-funded CoVentry project is investigating the early entry mechanism of CoV-SARS, CoV-SARS2 and hCoV-NL63, variants with different pathogenicity, which share the same cellular receptor angiotensin-converting enzyme 2. The project focuses on the characterisation of the viruses' interactions with the cell surface, including the bonds between individual CoV-S and single membrane components and multivalent interactions at the cell surface.
Objective
Coronaviruses (CoV) have been responsible for several severe viral outbreaks culminating in the current global pandemic. However, some viruses of this family are widespread and only cause mild conditions, like the common cold. The origin of the significant variation in the severity of the CoV-related diseases is still poorly understood. Recent studies have suggested that the strength of the interaction between the virus and the cell surface during the early stages of virus entry could play an important role. CoVs attachment to the plasma membrane is mediated by the specific interaction between the viral spike glycoprotein (CoV-S) and receptors found on the cell surface. In addition, several CoVs have been shown to interact with the cellular glycocalyx during the early attachment to the cell surface. In this proposal, I describe the study of the early entry mechanism of CoV-SARS, CoV-SARS2, and hCoV-NL63 which all target the same cellular receptor, angiotensin-converting enzyme 2, while strongly varying in their pathogenicity. The study focuses on the dynamics, kinetics and strength of the interaction of these viruses with the cell surface. It employs an incremental approach, from the study of the bond between individual CoV-S and single membrane components to multivalent interactions between the virion and the cell surface. A wide array of biophysical (e.g. single-particle tracking, and optical tweezer) and biological (e.g. viral pseudotypes) techniques are used, combining the host’s and my expertise. This multidisciplinary project will result in a unique and comprehensive characterisation of the interactions taking place during CoV-entry, it will elucidate the difference between viral species, and it will give insights into the origin of the observed differences in pathogenicity. In addition, this work will strengthen and expand my experience and network in the virology and biophysics fields, significantly improving my career prospects as an independent researcher.
Fields of science
- natural sciencesbiological sciencesmicrobiologyvirology
- medical and health scienceshealth sciencespublic healthepidemiologypandemics
- medical and health scienceshealth sciencesinfectious diseasesRNA virusescoronaviruses
- natural sciencesbiological sciencesbiophysics
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programme(s)
Funding Scheme
MSCA-IF-EF-ST - Standard EFCoordinator
901 87 Umea
Sweden