Over the course of the project, our primary focus has been to address the global issue of demineralization in dental hard tissues, particularly dentin hypersensitivity (DH). Our project, centered around the pioneering 'Deep Mineralization' method, has made significant strides in the biomimetic restoration of exposed dentin.
Key project activities included the experimental selection of peptides through Deep Directed Evolution, combining directed evolution with next-generation sequencing to generate extensive quantitative peptide datasets, often referred to as big data. Utilizing the machine learning-based predictive design platform, BioMInt, we successfully developed protein-derived peptides, refining our approach through high-throughput validation assays.
A milestone achievement of the project is establishing a peptide-guided remineralization methodology, Deep Mineralization. This innovative method mimics saliva's natural remineralization action, effectively sealing exposed dentin tubules by synthesizing an integrated mineral layer. Our recent publication, alongside several others, highlights the successful development of a peptide-based remineralization procedure. This procedure, demonstrated on extracted human teeth, showcases peritubular mineralization, ensuring structural, chemical, and thermal stability.
Looking beyond its immediate application to dentin hypersensitivity, Deep Mineralization holds promise for broader dental health. Its adaptability and scalability position it as a valuable asset in routine dental care practices, contributing not only to DH treatment but also to preventive dentistry on a larger scale.
As we reflect on the completed project timeline, marked by key milestones and achievements, our unwavering commitment remains towards realizing the ultimate goal of Deep Mineralization. The successful development of a practical and permanent solution for dentin hypersensitivity, as evidenced by our recent publication, marks a significant contribution to global dental health. Moving forward, we anticipate the transformative impact of our research on dental practices, potential commercialization avenues, and the advancement of preventive measures in oral healthcare.
