Descripción del proyecto
Nuevas enzimas para generar reacciones inéditas en la naturaleza
La catálisis enzimática y la mayor disponibilidad de las enzimas utilizadas en la síntesis química están logrando que la biocatálisis resulte interesante para producir moléculas quirales. A pesar de su potencial, las enzimas naturales solo utilizan una parte relativamente pequeña del espacio de reacción. Esto sugiere que en la biocatálisis solo puede emplearse eficazmente un número limitado de clases de reacciones estereocontroladas. Financiado por las Acciones Marie Skłodowska-Curie, el equipo del proyecto BIO-LIGHT investigará nuevas estrategias que deberían permitir a las enzimas catalizar reacciones asimétricas nuevas en la naturaleza, ampliando así los límites de reactividad de la biocatálisis. Para lograr su objetivo, el proyecto combinará la biocatálisis con la fotocatálisis, un método moderno de reactividad química que ofrece una posible forma de construir nuevas moléculas de modo sostenible.
Objetivo
Enzymatic catalysis plays a centrally important role for the preparation of chiral molecules and in the transition towards a more sustainable chemistry. In spite of their potential, natural enzymes use only a relatively small section of ‘reaction space’. This implies that only a limited number of stereocontrolled reaction classes, which are characterized by a similar reaction mechanism, can be effectively promoted. For example, a natural enzyme co-opted for new chemistry has the limitation that the new reactivity must be quite closely related to the naturally established catalytic function. The BIO-LIGHT project seeks to identify new strategies that allow enzymes to catalyse new-to-nature asymmetric reactions, thus expanding the reactivity boundaries of biocatalysis. To achieve this goal, we will combine biocatalysis with visible light photocatalysis, a modern strategy of chemical reactivity which offers a potent way to sustainably build new molecules. Specifically, we will explore the potential of native intermediates in the enzymes’ active sites to reach an excited state upon light absorption and enable new-to-nature enantioselective radical reactions. Light will be used to elicit mechanistic divergence within enzymes with an established ground-state reactivity, thus allowing them to catalyse completely different processes than those for which they evolved. The collaboration with an international pharma-company (Bayer AG) will facilitate the development of photo-enzymatic processes yielding chiral molecules adorned with biologically relevant scaffolds.
BIO-LIGHT combines the fellow’s experience in enzymatic catalysis and directed evolution and the host’s experience in photo-triggered asymmetric radical processes. The multi-cultural and intersectorial nature of this project will contribute to broaden the fellow’s competencies and will place him in a competitive position for the next career move.
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
43007 Tarragona
España