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Linking the Target of Rapamycin signalling to cell cycle progression during tuber initiation and its impact on potato yield parameters

Project description

Biomolecular mechanisms behind potato tuber growth and yield

The EU-funded TorCrop project will build on knowledge gained from model organisms to better understand the molecular mechanisms underlying tuber initiation and growth in potatoes. The research fellow will characterise cell proliferation and cell growth during tuber formation and explore the contribution of the nutrient-sensing target of rapamycin (TOR) pathway. They will also identify quantitative trait loci that define tuber size, which is connected to enhanced TOR activation. In addition, TorCrop will analyse allelic variations in identified candidate genes and link these to phenotypic variations available from breeding companies and in public databases. Finally, predictive mathematical models will be developed that describe cell size, number and changes throughout tuberisation in relation to tuber size and ultimately yield.

Objective

Potato tubers are nutrient storage organs that develop from underground stems called stolons through a process called tuberisation. This process is under the control of extrinsic and intrinsic signals, however, little is known about how these signals initiate and maintain tuber growth. We know that the first step is the initiation of cell division at the subapical region of the stolon, however, how this cell division is promoted and coordinated through developmental programs has not yet been studied. The TorCrop project will build on knowledge accumulated through fundamental research in model organisms to gain molecular insight in tuber initiation and growth. For this purpose, we will induce tuber growth by sugar and characterise the cell proliferation and cell growth during tuber formation. As next step, we will explore how the nutrient sensing Target of Rapamycin (TOR) pathway contributes to this and is there any hierarchical connection between TOR and the “tuberigen” signal. Then, we will identify quantitative trait loci (QTLs) that define tuber size, size distribution which is connected to enhanced TOR activation. We will also analyse allelic variations in identified candidate genes and link these to phenotypic variations available from breeding companies and in public databases. Finally, we will create predictive mathematical models that describe cell size, number and changes throughout tuberisation in relation to tuber size and ultimately yield. Therefore, the project will provide a better understanding on organ development and ultimately yield in crops by providing knowledge at molecular and systems biology levels.

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Topic(s)

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2020

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Coordinator

WAGENINGEN UNIVERSITY
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 187 572,48
Address
DROEVENDAALSESTEEG 4
6708 PB Wageningen
Netherlands

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Region
Oost-Nederland Gelderland Veluwe
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 187 572,48
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