A binary sensor with single-molecule digit to discriminate biofluids enclosing zero or at least one biomarker

Project Information

NoOne

Grant agreement ID: 101040383

DOI

10.3030/101040383

EC signature date 18 March 2022

Start date 1 April 2022

End date 31 March 2027

Funded under

• European Research Council (ERC)

Total cost

€ 2 499 579,00

EU contribution

€ 2 499 579,00

Coordinated by

UNIVERSITA DEGLI STUDI DI BARI ALDO MORO
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (34)

Inside out: Exploring edible biocatalytic biosensors for health monitoring

Author(s): Verdiana Marchianò, Angelo Tricase, Alessandra Cimino, Blanca Cassano, Michele Catacchio, Eleonora Macchia, Luisa Torsi, Paolo Bollella
Published in: Bioelectrochemistry, Issue 161, 2025, ISSN 1567-5394
Publisher: Elsevier BV
DOI: 10.1016/j.bioelechem.2024.108830

Long-term electrical characteristics of a poly-3-hexylthiophene water-gated thin-film tran-sistor

Author(s): Luukkonen, A; Tewari, A; Björkström, K; Ghafari, AM; Macchia, E; Torricelli, F; Torsi, L; Österbacka, R.*
Published in: Organic Electronics, Issue 120, 2023, ISSN 1566-1199
Publisher: Elsevier BV
DOI: 10.1016/j.orgel.2023.106844

Implementation of Experimental Design Techniques to Optimize Immunoglobulins Detection with Ultrasensitive Sandwich Immunoassays

Author(s): Cecilia Scandurra; Paolo Bollella; Ronald Österbacka; Francesco Leonetti; Eleonora Macchia; Luisa Torsi
Published in: Advanced Sensor Research, Issue 1, 2022, ISSN 2751-1219
Publisher: Wiley
DOI: 10.1002/adsr.202200009

Extended Work Function Shift of Large-Area Biofunctionalized Surfaces Triggered by a Few Single-Molecule Affinity Binding Events

Author(s): Di Franco, C., Macchia, E., Sarcina, L., Ditaranto, N., Khaliq, A., Torsi, L.,* Scamarcio, G.
Published in: Advanced Interface Materials, Issue 2201829, 2023, ISSN 2196-7350
Publisher: WILEY
DOI: 10.1002/admi.202201829

Ultrasensitive detection of 2,4-dichlorophenoxyacetic acid by inhibiting alkaline phosphatase immobilized onto a highly porous gold nanocoral electrode

Author(s): Angelo Tricase, Michele Catacchio, Verdiana Marchianò, Eleonora Macchia, Paolo Bollella, Luisa Torsi
Published in: Nanoscale, 2025, ISSN 2040-3364
Publisher: Royal Society of Chemistry (RSC)
DOI: 10.1039/d4nr04857a

Perspectives on systematic optimization of ultrasensitive biosensors through experimental design

Author(s): Mariapia Caputo, Angelo Tricase, Verdiana Marchianò, Cecilia Scandurra, Matteo Piscitelli, Lucia Sarcina, Michele Catacchio, Cinzia Di Franco, Paolo Bollella, Luisa Torsi, Eleonora Macchia
Published in: Journal of Materials Chemistry C, Issue 12, 2024, ISSN 2050-7526
Publisher: Royal Society of Chemistry (RSC)
DOI: 10.1039/d4tc02131b

Multistep Fabrication of OECTs via Direct Ink Writing, With Performance Analysis Based on a Novel Measurement Protocol

Author(s): Amir Mohammad Ghafari; Oskar Backman; Emil Rosqvist; Chunlin Xu; Luisa Torsi; Eleonora Macchia; Ronald Österbacka
Published in: Advanced Electronic Materials, 2024, ISSN 2199-160X
Publisher: Wiley
DOI: 10.1002/aelm.202400742

Rapid and Ultra‐Sensitive SARS‐CoV‐2 Subgenomic RNA Detection Using Single‐Molecule With a Large Transistor‐SiMoT Bioelectronic Platform

Author(s): Eleonora Macchia, Anna Maria D'Erchia, Mariapia Caputo, Angelica Bianco, Claudia Leoni, Francesca Intranuovo, Cecilia Scandurra, Lucia Sarcina, Cinzia Di Franco, Paolo Bollella, Gaetano Scamarcio, Luisa Torsi, Graziano Pesole
Published in: Advanced Electronic Materials, 2025, ISSN 2199-160X
Publisher: Wiley
DOI: 10.1002/aelm.202400908

Electric Field Cycling of Physisorbed Antibodies Reduces Biolayer Polarization Dispersion

Author(s): Cinzia Di Franco, Eleonora Macchia, Michele Catacchio, Mariapia Caputo, Cecilia Scandurra, Lucia Sarcina, Paolo Bollella, Angelo Tricase, Massimo Innocenti, Riccardo Funari, Matteo Piscitelli, Gaetano Scamarcio, Luisa Torsi
Published in: Advanced Science, Issue 12, 2025, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202412347

High voltage flexible glucose/O2 fully printed hydrogel-based enzymatic fuel cell.

Author(s): Marchiano, V; Tricase, A; Ditaranto, N; Macchia, E; d'Ingeo, S; Di Franco, C; Scamar-cio, G; Torsi, L; Bollella, P.*
Published in: Journal Of Physics D-Applied Physics, Issue 57 135503, 2024, ISSN 1361-6463
Publisher: Purpose-Led Publishing
DOI: 10.1088/1361-6463/ad1850

Why a diffusing single-molecule can be detected in few minutes by a large capturing bioelectronic interface.

Author(s): Macchia, E., De Caro, L., Torricelli, F., Di Franco, C., Mangiatordi, G.F., Scamarcio, G.*, and Torsi, L.*
Published in: Advanced Science, Issue 9, 2022, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202104381

A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

Author(s): Genco, E., Modena, F., Sarcina, L., Björkström, K., Brunetti, C., Caironi, M., Caputo, M., Demartis, V.M., Di Franco, C., Frusconi, G., Haeberle, L., Larizza, P., Mancini, M.T., Österbacka, R., Reeves, W., Scamarcio, G., Scandurra, C., Wheeler, M., Ca
Published in: Advanced Materials, Issue 35, 2023, ISSN 0935-9648
Publisher: Wiley
DOI: 10.1002/ADMA.202304102

A handheld intelligent single-molecule binary bioelectronic system for fast and reliable immunometric point-of-care testing

Author(s): Macchia, E., Kovacs-Vajna, Z.M., Loconsole, D., Sarcina, L., Redolfi, M., Chironna, M., Torricelli, F., Torsi, L.
Published in: Science Advances, Issue 27, 2022, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1126/SCIADV.ABO0881

Plasmonic Single‐Molecule Affinity Detection at 10⁻²⁰ Molar

Author(s): Eleonora Macchia, Cinzia Di Franco, Cecilia Scandurra, Lucia Sarcina, Matteo Piscitelli, Michele Catacchio, Mariapia Caputo, Paolo Bollella, Gaetano Scamarcio, Luisa Torsi
Published in: Advanced Materials, Issue 37, 2025, ISSN 0935-9648
Publisher: Wiley
DOI: 10.1002/adma.202418610

Tailoring Water-Based Graphite Conductive Ink Formulation for Enzyme Stencil-Printing: Experimental Design to Enhance Wearable Biosensor Performance

Author(s): Marchianò, V., Tricase, A., Caputo, M., Farinini, E., Leardi, R., Imbriano, A., Leech, D., Kidayaveettil, R., Gentile, L., Torsi, L.*, Macchia, E.*, Bollella, P.*
Published in: Chemistry of Materials, Issue 36, 2023, ISSN 0897-4756
Publisher: American Chemical Society
DOI: 10.1021/acs.chemmater.3c02229

Ultrasensitive and Highly Selective o-Phenylenediamine Molecularly Imprinted Polymer for the Detection of 2,4-Dichlorophenoxyacetic Acid

Author(s): Angelo Tricase, Verdiana Marchianò, Eleonora Macchia, Nicoletta Ditaranto, Luisa Torsi, Paolo Bollella
Published in: Electrochimica Acta, Issue 494, 2024, ISSN 0013-4686
Publisher: Elsevier BV
DOI: 10.1016/j.electacta.2024.144430

β‐Sheets Orientation in Physisorbed Protein Layers

Author(s): Matteo Piscitelli; Diellza Bajrami; Cinzia Di Franco; Lucia Sarcina; Michele Catacchio; Eleonora Macchia; Luisa Torsi; Boris Mizaikoff; Gaetano Scamarcio
Published in: Advanced Materials Interfaces, Issue 12, 6, 2024, ISSN 2196-7350
Publisher: WIELY
DOI: 10.1002/admi.202400867

A large‑area organic transistor with 3D‑printed sensing gate for noninvasive single‑molecule detection of pancreatic mucinous cyst markers

Author(s): Sarcina, L., Viola, F., Modena, F., Picca, R.A., Bollella, P., Di Franco, C., Cioffi, N., Caironi, M., Österbacka, R., Esposito, I., Scamarcio, G., Torsi, L., Torricelli, F., Macchia, E.*
Published in: Analytical and Bioanalytical Chemistry, Issue 414, 2022, ISSN 1618-2650
Publisher: Springer
DOI: 10.1007/s00216-022-04040-4

Analysis of Clinical Samples of Pancreatic Cyst's Lesions with A Multi-Analyte Bioelectronic Simot Array Benchmarked Against Ultrasensitive Chemiluminescent Immunoassay

Author(s): Scandurra, C., Björkström, K., Caputo, M., Sarcina, L., Genco, E., Modena, F., Viola, FA., Brunetti, C., Kovács-Vajna, Z.M., Di Franco, C., Haeberle, L., Larizza, P., Mancini, M.T., Österbacka, R., Reeves, W., Scamarcio, G., Wheeler, M., Caironi, M.,
Published in: Advanced Science, Issue 2308141, 2024, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202308141

Stability of thiol-based self-assembled monolayer functionalized electrodes in EG-OFET-based applications.

Author(s): Tewari, A., Björkström, K., Ghafari, A.M., Macchia, E., Torsi, L., Österbacka, R.*
Published in: FlatChem, Issue 42, 2023, ISSN 2452-2627
Publisher: Elsevier B.V.
DOI: 10.1016/J.FLATC.2023.100553

Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

Author(s): Scandurra,C., Kim Björkström, K., Sarcina, L., Imbriano, A., Di Franco, C., Öster-backa, R., Bolella, P., Scamarcio, G., Torsi,L.,* Macchia, E
Published in: Advanced Materials Technologies,, Issue 8, 2023, ISSN 2365-709X
Publisher: Wiley
DOI: 10.1002/admt.202201910

Controlling the Binding Efficiency of Surface Confined Antibodies through the Design of Mixed Self-Assembled Monolayers

Author(s): Lucia Sarcina, Pietro Delre, Giovanni Graziano, Angela Stefanachi, Davide Blasi, Rosaria A. Picca, Cinzia Di Franco, Francesco Leonetti, Gaetano Scamarcio, Paolo Bollella, Giuseppe F. Mangiatordi,* Eleonora Macchia,* and Luisa Torsi*
Published in: Advanced Materials Interfaces, Issue 10, 2023, ISSN 2196-7350
Publisher: Wiley
DOI: 10.1002/admi.202300017

Label-free electronic detection of peptide post-translational modification with functional enzyme-driven assay at the physical limit

Author(s): Macchia, Eleonora; Björkström, Kim; Tewari, Amit; Eskonen, Ville; Luukkonen, Axel; Ghafari, Amir Mohammad; Sarcina, Lucia; Caputo, Mariapia; Tong-Ochoa, Natalia; Kopra, Kari; Pettersson, Fredrik; Gounani, Zahra; Torsi, Luisa; Härmä, Harri; Österbacka, Ronald
Published in: Cell Reports Physical Science, Issue 101874, 2024, ISSN 2666-3864
Publisher: Cell Press
DOI: 10.1016/j.xcrp.2024.101874

Kelvin probe force microscopy on patterned large-area biofunctionalized surfaces: a reliable ultrasensitive platform for biomarker detection

Author(s): Cinzia Di Franco; Matteo Piscitelli; Eleonora Macchia; Cecilia Scandurra; Michele Catacchio; Luisa Torsi; Gaetano Scamarcio
Published in: Journal of Materials Chemistry C, Issue 1, 2023, ISSN 2050-7526
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3tc03110a

Piercing the Shadows: Exploring the Influence of Signal Preprocessing on Interpreting Ultrasensitive Bioelectronic Sensor Data

Author(s): Mariapia Caputo, Lucia Sarcina, Cecilia Scandurra, Michele Catacchio, Matteo Piscitelli, Cinzia Di Franco, Paolo Bollella, Gaetano Scamarcio, Luisa Torsi, Eleonora Macchia
Published in: ChemPlusChem, Issue 90, 2025, ISSN 2192-6506
Publisher: Wiley
DOI: 10.1002/cplu.202400520

Graphene‐Based Opto‐Electronic Platform for Ultra‐Sensitive Biomarker Detection at Zeptomolar Concentrations

Author(s): Matteo Piscitelli; Cinzia Di Franco; Giuseppe Valerio Bianco; Giovanni Bruno; Eleonora Macchia; Luisa Torsi; Gaetano Scamarcio
Published in: Small Methods, 2025, ISSN 2366-9608
Publisher: WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.202402026

Chemical Reviews

Author(s): Macchia, E., Torricelli, F., Bollella, P., Sarcina, L., Tricase, A., Di Franco, C., Österbacka, R., Kovács-Vajna, Z.M., Scamarcio, G., Torsi, L.*
Published in: Chemical Reviews, Issue 122, 2022, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.1c00290

Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors

Author(s): Tricase, A., Imbriano, A., Valentino, M., Ditaranto, N., Macchia, E., Di Franco, C., Kidayaveettil, R., Leech, D., Piscitelli, M., Scamarcio, G., Perchiazzi, G., Torsi, L.* Paolo Bollella, P.
Published in: Advanced Sensor Research, Issue 3, 2023, ISSN 2751-1219
Publisher: Wiley
DOI: 10.1002/adsr.202300036

A stable physisorbed layer of packed capture antibodies for high-performance sensing applications

Author(s): Sarcina, L., Scandurra, C., Di Franco, C., Caputo, M., Catacchio, M., Bollella, P., Scamarcio, G., Macchia, E., Torsi, L.*
Published in: Journal of Materials Chemistry C, Issue 11, 2023, ISSN 2050-7526
Publisher: The Royal Society of Chemistry
DOI: 10.1039/D3TC01123B

Large-Area Interfaces for Single-Molecule Label-free Bioelectronic Detection

Author(s): Eleonora Macchia, Fabrizio Torricelli, Paolo Bollella, Lucia Sarcina, Angelo Tricase, Cinzia Di Franco, Ronald Österbacka, Zsolt M. Kovács-Vajna, Gaetano Scamarcio, Luisa Torsi
Published in: Chemical Reviews, Issue 122, 2023, ISSN 0009-2665
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACS.CHEMREV.1C00290

Self-powered wearable biosensor based on stencil-printed carbon nanotube electrodes for ethanol detection in sweat

Author(s): Verdiana Marchianò; Angelo Tricase; Eleonora Macchia; Paolo Bollella; Luisa Torsi
Published in: Analytical and Bioanalytical Chemistry, Issue 416, 2024, ISSN 1618-2650
Publisher: Springer
DOI: 10.1007/S00216-024-05467-7

Fast and Reliable Electronic Assay of a Xylella fastidiosa Single Bacterium in Infected Plants Sap

Author(s): Sarcina, L.; Macchia,*E; Loconsole, G.; D’Attoma, G.; Bollella, P.; Catacchio M.; Leo-netti, F.; Di Franco, C.; Elicio, V.; Scamarcio, G.; Palazzo, G.; Boscia, D.; Saldarelli, S.; and Torsi, L
Published in: Advanced Science 2022, Issue 2203900, 2022, ISSN 2198-3844
Publisher: WILEY
DOI: 10.1002/advs.202203900

Point-Of-Care Ultra-Portable Single-Molecule Bioassays for One-Health

Author(s): Macchia, E., Torricelli, F., Caputo, M., Sarcina, L., Scandurra, C., Bollella, P., Catac-chio, M., Piscitelli, M., Di Franco, C., Scamarcio, G., Torsi, L.
Published in: Advanced Materials, Issue VOL 36 (13), 2023, ISSN 1521-4095
Publisher: WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.202309705

Experimental design of stencil-printed high-performance organic electrochemical transistors

Author(s): Amir Mohammad Ghafari; Michele Catacchio; Emil Rosqvist; Axel Luukkonen; Anni Eklund; Kim Björkström; Paolo Bollella; Luisa Torsi; Eleonora Macchia; Ronald Österbacka
Published in: Advanced Materials, Issue 4, 2023, ISSN 2633-5409
Publisher: The Royal Society of Chemistry
DOI: 10.1039/d3ma00888f

Conference proceedings (3)

Physical Modelling of Large-Area Single-Molecule Organic Transistors

Author(s): Fabrizio Torricelli, Eleonora Macchia, Paolo Bollella, Cinzia Di Franco, Zsolt M. Kovacs-Vajna, Gaetano Scamarcio, Luisa Torsi
Published in: 2022 IEEE International Conference on Flexible and Printable Sensors and Systems (FLEPS), 2022
Publisher: IEEE
DOI: 10.1109/FLEPS53764.2022.9781507

Large-area bio-electronic sensors for early detection of pancreatic-biliary cancer protein markers

Author(s): Lucia Sarcina, Fabrizio Viola, Francesco Modena, Paolo Bollella, Mario Caironi, Irene Esposito, Luisa Torsi, Fabrizio Torricelli, Eleonora Macchia
Published in: 2022 IEEE International Conference on Flexible and Printable Sensors and Systems (FLEPS), 2022
Publisher: IEEE
DOI: 10.1109/FLEPS53764.2022.9781550

Flexible Carbon-based electrodes for amperometric biosensor development

Author(s): Angelo Tricase, Verdiana Marchianò, Eleonora Macchia, Reshma Kidayaveettil, Donal Leech, Gaetano Scamarcio, Gaetano Perchiazzi, Luisa Torsi, Paolo Bollella
Published in: 2024 IEEE International Conference on Flexible and Printable Sensors and Systems (FLEPS), 2024
Publisher: IEEE
DOI: 10.1109/FLEPS61194.2024.10603574