Description du projet
Étude d’une nouvelle classe de coiffes d’ARN et de leur rôle dans la réponse cellulaire aux infections et au stress
Le projet StressRNaction, financé par l’UE, étudie le rôle des dinucléosides polyphosphates (NpnN), des coiffes d’ARN non canonique récemment découvertes, dans les cellules procaryotes et eucaryotes. Ces coiffes, présentes à l’extrémité 5’ des nucléotides spécialement modifiés, sont vitales pour la création de l’ARN messager mature et pour la traduction lors de la synthèse des protéines. Les coiffes NpnN méthylées découvertes par les chercheurs du projet stabilisent l’ARN dans Escherichia coli en phase stationnaire et peuvent être présentes dans les cellules de mammifères. L’objectif du projet est d’élaborer des techniques de capture sélective pour identifier les séquences NpnN-ARN et les partenaires en interaction. Le projet vise à comprendre le métabolisme des coiffes NpnN, leur rôle dans la réponse immunitaire aux virus et aux bactéries, ainsi que les conditions de stress dans les cellules procaryotes et eucaryotes.
Objectif
The goal of this project is to understand the role of non-canonical RNA caps (mainly dinucleoside polyphosphates = NpnNs) in prokaryotes and eukaryotes. The 5' termini of the RNA are critical structures and are the least characterized among RNA modifications. In this project, we will develop selective capturing techniques for identification of NpnN-RNA sequences and identify the interacting partners of NpnN-RNA. Furthermore, we will reveal their metabolism and their role in cellular reaction to stress conditions in prokaryotes and eukaryotes.
Until recently only canonical structures, NAD or CoA have been known as 5' RNA caps. We discovered an entirely new class of 5' RNA caps - dinucleoside polyphosphates (NpnN) in prokaryotic and eukaryotic cells. Based on our preliminary data we know that methylated NpnN caps stabilize RNA of E. coli in the stationary phase and that some NpnN caps can also be found in mammalian cells. We do not yet know [1] the sequence of RNAs capped with NpnNs, [2] how many types of NpnN RNA caps exist in eukaryotes, [3] whether RNA stabilization is their only role, [4] why there are so many types of NpnN RNA caps (we identified nine in E. coli), [5] whether NpnN-RNA can be translated, etc.
The role of free NpnNs, identified fifty years ago, is yet to be elucidated. NpnNs are called alarmones, as their concentration increases under stress conditions. The mechanism by which the alarm is recognized in cells is unknown. I presume that their cellular effects are mediated by the RNA, where they serve as RNA caps. As such, they become an important part of RNA metabolism and can be recognized by various RNA interacting proteins, triggering additional effects in cellular metabolism. The presented project has the potential to solve the puzzle of the role of NpnNs and clarify the connection between RNA metabolism and immune response or virulence factors of viruses and bacteria.
Champ scientifique
Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Thème(s)
Régime de financement
HORIZON-AG - HORIZON Action Grant Budget-BasedInstitution d’accueil
16610 Praha 6
Tchéquie