Objective
"The ideal microscopy experiment would take place in native cells without genetic engineering, with 3-dimensional resolution on the single molecule scale (<10 nm) by observing the endogenous molecule itself. I propose the introduction and use of deuterium (“deuterON”) as a general method for a multimodal approach, to i) synthesize a first-in-class deuterated cyanine (Cy) fluorophore for super-resolution imaging, ii) to design and test deuterated, next-level photoswitches to restore vision, and iii) use these probes and deuterated drugs for direct and bioorthogonal, spectroscopic imaging. In particular, deuterated Cys will allow stochastic reconstruction microscopy (STORM) by using near infra-red light to break new ground in protein localization in live tissue, opening the gates for thick sample imaging (~100 m axial) with retained super-resolution (~20 nm). In addition, deuterated azobenzene photoswitches will be designed to finally reach the indispensable, and to-date unobtained light sensitivity to remote control neural signalling in vivo. Lastly, the use of ""label-free"" labelling and imaging will be explored with deuterated drugs to observe drug uptake and metabolism by utilizing the unique properties of the carbon-deuterium bond in Raman spectroscopy. Coupled to a confocal microscope, deuterated drugs will be tracked in native and live cells, without any genetic engineering strategies, and on the molecule of interest itself, reducing perturbations and artefacts to a minimum. This ground-breaking approach holds promise to be generalizable to Chemical Biology disciplines, and as an unconventional, yet attractive and powerful method to design and synthesize next generation small molecule probes. Developing a pipeline for these aims will be a game changer, with ramifications for the life sciences, cell biology, drug development and with prospective translational impact."
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2021-STG
See all projects funded under this callHost institution
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
12489 Berlin
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.