Objective
Self-assembly is a fundamental foundation of life, but what about co-assembly? The main goal of BiFOLDOME is to decipher co-assembly to understand self-assembly. Amyloids were assumed to be assembled by one type of protein, but our recent elucidation of the first 1:1 hetero-amyloid structure (the RIPK1-RIPK3 necrosome core) suggests that amyloids composed of two distinct proteins playing key roles in health and disease may be common. In fact, a viral protein (M45) can displace one partner (RIPK1) to form a distinct 1:1 hetero-amyloid (M45-RIPK3). Taking a leaf from the viral playbook, this means that for a given self-assembling sequence there may be a mating sequence driving the preferential 1:1 co-assembly of the two. Thus, understanding what drives the preferential formation of co-assembled forms over conventional self-assembled species will afford an entirely new vision on assembly processes transversal to all fields of knowledge. BiFOLDOME is organized around three different levels of complexity: (1) characterizing the formation, structure and energetics of representative paradigms of 1:1 co-assembled amyloids using solution and solid-state NMR spectroscopies, and energy calculations, featuring novel technical innovations that we will develop. This will provide the basis for self-assembly by delivering a firm understanding of co-assembly. (2) Applying the fundamental knowledge from (1) to the manipulation of self-assembled, disease-associated proteins using the powerful concept of 1:1 co-assembly. (3) Going beyond the state of the art by developing a new methodology to study the assembly of biomolecular condensates. The approach, which I call “optoNMR”, will enable controlled, light-triggered self- and co-assembly of proteins within the NMR tube, opening new avenues to discern between alternative hypotheses for condensate formation and hardening in real-time and at high resolution, or for sensitive detection using hyperpolarization schemes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2021-STG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
28006 MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.