During this period, we have made significant technological advancements and produced scientific insights that have been well-received by the research community. Our main achievements focus on two key areas: linking gene dosage changes to regulatory networks and understanding how genetic and environmental factors influence gene expression.
In our work on gene dosage and regulatory networks, we successfully developed, tested and published a new CRISPR-based method for modulating gene expression. This approach allows for precise control of gene activity, helping us understand how changes in gene dosage affect other genes, particularly those involved in diseases. Our findings reveal that these effects are nonlinear, complex and vary by gene, providing valuable insights for future research.
Additionally, we created and published a new tool and data resource that helps researchers map the direct targets of transcription factors in specific cell types. This resource is user-friendly and effective, making it a valuable contribution to the field.
In another project, we explored how both genetics and environmental factors influence gene expression. We introduced a new method that improves the identification of biologically significant changes in gene expression, especially in response to environmental triggers and physiological changes such as disease. This method enhances our ability to identify key pathways involved in cellular responses, offering a clearer understanding of how our genes react to the environment.
Overall, our work lays a strong foundation for future research and provides new tools and insights that will benefit the broader scientific community.
