Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX

Informations projet

PHOENIX

N° de convention de subvention: 101043985

DOI

10.3030/101043985

Date de signature de la CE 25 Juillet 2022

Date de début 1 Avril 2023

Date de fin 31 Mars 2028

Financé au titre de

• European Research Council (ERC)

Coût total

€ 2 832 500,00

Contribution de l’UE

€ 2 832 500,00

Coordonné par

UPPSALA UNIVERSITET
Sweden

Periodic Reporting for period 1 - PHOENIX (Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX)

Période du rapport: 2023-04-01 au 2025-09-30

Résumé du contexte et des objectifs généraux du projet

The project 'PHOENIX', or 'Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX' will further expand the droplet microfluidics toolbox that the Tenje lab has pioneered during the last ten years, to gain control of the cellular architecture of organoid structure by using integrated acoustics. For the project, we will collaborate with researchers from Leiden University Medical Center in the Netherlands, and researchers at Karolinska Institute and Lund University in Sweden.

PHOENIX will allow the Tenje lab to:

- Develop biocompatible acoustofluidic methods for manipulation of stem cells in viscous media
- Acquire spatio-temporal control of the organoid differentiation process via microfluidic solutions
- Integrate vascular networks in brain organoids using microfabrication techniques
- Validate added benefits and improved functionality of brain organoids generated on-chip

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

To date, we have established a theoretical framework for acoustic manipulation of viscous droplets so that we now have the tools to target system design and fabrication. We are exploring the use of two-photon polymerisation of an acoustic trapping array that can be used to withhold organoids against a flow in a microfluidic device. In this way, we hypothesise we will be able to control the differentiation processes better and increase reproducibility for generation of brain organoids.

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

We are currently finalising the theoretical work performed into a scientific publication to share with the research community that have an interest in two-phase acoustofluidics. We have already published the work on dynamics of droplet formation using non-Newtonian fluids.
Once the acoustic trapping array is established, we will connect with other research groups in the organoid field to investigate possibilities to control different process of other organ models in addition to brain organoids.

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