Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX

Información del proyecto

PHOENIX

Identificador del acuerdo de subvención: 101043985

DOI

10.3030/101043985

Fecha de la firma de la CE 25 Julio 2022

Fecha de inicio 1 Abril 2023

Fecha de finalización 31 Marzo 2028

Financiado con arreglo a

• European Research Council (ERC)

Coste total

€ 2 832 500,00

Aportación de la UE

€ 2 832 500,00

Coordinado por

UPPSALA UNIVERSITET
Sweden

Periodic Reporting for period 1 - PHOENIX (Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX)

Período documentado: 2023-04-01 hasta 2025-09-30

Resumen del contexto y de los objetivos generales del proyecto

The project 'PHOENIX', or 'Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX' will further expand the droplet microfluidics toolbox that the Tenje lab has pioneered during the last ten years, to gain control of the cellular architecture of organoid structure by using integrated acoustics. For the project, we will collaborate with researchers from Leiden University Medical Center in the Netherlands, and researchers at Karolinska Institute and Lund University in Sweden.

PHOENIX will allow the Tenje lab to:

- Develop biocompatible acoustofluidic methods for manipulation of stem cells in viscous media
- Acquire spatio-temporal control of the organoid differentiation process via microfluidic solutions
- Integrate vascular networks in brain organoids using microfabrication techniques
- Validate added benefits and improved functionality of brain organoids generated on-chip

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

To date, we have established a theoretical framework for acoustic manipulation of viscous droplets so that we now have the tools to target system design and fabrication. We are exploring the use of two-photon polymerisation of an acoustic trapping array that can be used to withhold organoids against a flow in a microfluidic device. In this way, we hypothesise we will be able to control the differentiation processes better and increase reproducibility for generation of brain organoids.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

We are currently finalising the theoretical work performed into a scientific publication to share with the research community that have an interest in two-phase acoustofluidics. We have already published the work on dynamics of droplet formation using non-Newtonian fluids.
Once the acoustic trapping array is established, we will connect with other research groups in the organoid field to investigate possibilities to control different process of other organ models in addition to brain organoids.

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