Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX

Projektinformationen

PHOENIX

ID Finanzhilfevereinbarung: 101043985

DOI

10.3030/101043985

EK-Unterschriftsdatum 25 Juli 2022

Startdatum 1 April 2023

Enddatum 31 März 2028

Finanziert unter

• European Research Council (ERC)

Gesamtkosten

€ 2 832 500,00

EU-Beitrag

€ 2 832 500,00

Koordiniert durch

UPPSALA UNIVERSITET
Sweden

Periodic Reporting for period 1 - PHOENIX (Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX)

Berichtszeitraum: 2023-04-01 bis 2025-09-30

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

The project 'PHOENIX', or 'Paving the way for High-throughput Organoid ENgineering using Integrated acoustiX' will further expand the droplet microfluidics toolbox that the Tenje lab has pioneered during the last ten years, to gain control of the cellular architecture of organoid structure by using integrated acoustics. For the project, we will collaborate with researchers from Leiden University Medical Center in the Netherlands, and researchers at Karolinska Institute and Lund University in Sweden.

PHOENIX will allow the Tenje lab to:

- Develop biocompatible acoustofluidic methods for manipulation of stem cells in viscous media
- Acquire spatio-temporal control of the organoid differentiation process via microfluidic solutions
- Integrate vascular networks in brain organoids using microfabrication techniques
- Validate added benefits and improved functionality of brain organoids generated on-chip

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

To date, we have established a theoretical framework for acoustic manipulation of viscous droplets so that we now have the tools to target system design and fabrication. We are exploring the use of two-photon polymerisation of an acoustic trapping array that can be used to withhold organoids against a flow in a microfluidic device. In this way, we hypothesise we will be able to control the differentiation processes better and increase reproducibility for generation of brain organoids.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

We are currently finalising the theoretical work performed into a scientific publication to share with the research community that have an interest in two-phase acoustofluidics. We have already published the work on dynamics of droplet formation using non-Newtonian fluids.
Once the acoustic trapping array is established, we will connect with other research groups in the organoid field to investigate possibilities to control different process of other organ models in addition to brain organoids.

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