Descripción del proyecto
Caracterización de las infecciones víricas tempranas
Después del acceso inicial a sus hospedadores, los virus se propagan de forma distinta en células y tejidos diferentes. Se desconoce lo que sucede en las etapas más tempranas de la infección celular debido a los retos técnicos que plantea la detección y el análisis de una carga vírica tan pequeña. Sobre la base del desarrollo de una tecnología pionera de obtención de imágenes para la detección de virus de ARN monocatenario positivo, el proyecto VirIm, financiado con fondos europeos, ampliará las capacidades de la tecnología para abordar el grupo más complejo de los virus de ARN negativo. Centrándose en el virus respiratorio sincicial, que puede resultar mortal en lactantes y adultos vulnerables y carece de un tratamiento eficaz, la mejora del conocimiento del equipo acerca de la propagación temprana del virus y de su impacto allanará el camino hacia tratamientos eficaces.
Objetivo
Viruses are simple replicative units built from two components: a nucleic acid and a small number of associated proteins. Upon entering a cell, viruses co-opt the hosts machineries to copy their genetic material, while shutting down the hosts antiviral immune response. The success of viral infection differs dramatically from cell to cell through poorly understood mechanisms, resulting in heterogenous propagation through tissues, and ultimately, heterogeneous disease progression.
Although viruses have been studied for decades, the earliest steps of cellular infection have remained hidden, because only a few viral molecules are present at this stage, which presents formidable challenges for molecular analyses. Yet these initial events are critical for achieving a successful infection; transcription, translation and replication must be perfectly balanced to rapidly scale virus production before antiviral signalling pathways are activated. To overcome this barrier, we recently developed a first-in-kind imaging technology for simple positive-sense single stranded RNA (+ssRNA) viruses that transforms our ability to visualize early viral infection processes.
In this proposal, we will expand our single–molecule toolbox to gain molecular insights into early viral infection of the more complex group of negative-sense RNA (-ssRNA) viruses. We will focus on the respiratory syncytial virus (RSV), a -ssRNA virus which can be deadly in infants and vulnerable adults, and lacks effective treatments. Specifically, we will determine i) how viral transcription and replication are coordinated on single viral genomic RNA molecules to optimize early viral propagation, ii) what causes the early viral infection heterogeneity, and iii) how heterogeneity in early viral infection impacts infection outcome. Using our novel approaches, we will gain a deep understanding of viral biology, which will eventually inform therapeutic interventions.
Ámbito científico
Palabras clave
Programa(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régimen de financiación
HORIZON-ERC - HORIZON ERC GrantsInstitución de acogida
1011 JV AMSTERDAM
Países Bajos