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CORDIS - Resultados de investigaciones de la UE
CORDIS

Long-term Microphysiological Sample Imaging for Evaluation of Polypharmacy in Liver

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Sample preparation and imaging protocols in drug treatment (se abrirá en una nueva ventana)

Pilot experiments of AFM in combination with FluidFM will be performed in order to assess the local response of LSEC to drugs and correlate dynamic changes with SIM

Report on correlation of images between AFM/FluidFM/SIM (se abrirá en una nueva ventana)

Optical nanoscopy data will be directly overlaid by atomic force microscopy height and elastic modulus maps in software in order to assess correlative effects

Updated plan for dissemination and exploitation including communication activities (se abrirá en una nueva ventana)

An updated plan for dissemination and exploitation including communication activities will be prepared and provided by this deliverable.

Data Management Plan (se abrirá en una nueva ventana)

A Data Management Plan will be prepared and submitted for the entire project

Evaluation report of the near-IR sensitive sCMOS camera (se abrirá en una nueva ventana)

This deliverable with provide a detailed report concerning the evaluation of the performance of the near-IR sensitive sCMOS camera developed within the project, incl. spectral sensitivity and read noise data.

Protocol for culture of 3D-liver samples within MPS system (se abrirá en una nueva ventana)

This deliverable with provide a detailed protocol about the conditions required for the successful culture of 3D-liver samples within the micro physiological systems unit.

Plan for dissemination and exploitation including communication activities (se abrirá en una nueva ventana)

A detailed issemination exploitation and communication plan will be prepared and submitted in this deliverable

Design & protocol hardware/assays of real-time LSEC porosity (se abrirá en una nueva ventana)

This deliverable with provide a detailed report on the design and the performance of the hardware and the assays achieved with this hardware to measure liver sinusoidal endothelial cell (LSEC) porosity in realtime.

High-throughput liver function assays linked to LSEC porosity (se abrirá en una nueva ventana)

This deliverable with provide detailed protocols for high-throughput liver function assays directly linked to real-time readouts of liver sinusoidal endothelial cell (LSEC) porosity.

RP2 update of the DMP (se abrirá en una nueva ventana)

An update of the data management for the 2nd reporting period will be provided in this deliverable.

Protocol for culture of LSEC within the microfluidic hardware (se abrirá en una nueva ventana)

This deliverable with provide a detailed protocol describing the culture of liver sinusoidal endothelial cells (LSEC) within the microfluidic hardware.

Project website (se abrirá en una nueva ventana)

The project website will be prepared and filled with content for public dissemination of the project its goals and its planned accomplishments

Publicaciones

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure (se abrirá en una nueva ventana)

Autores: Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger & Peter A. G. McCourt
Publicado en: Scientific Reports, Edición 13, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/S41598-023-40227-0

High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components (se abrirá en una nueva ventana)

Autores: Henning Ortkrass, Jasmin Schürstedt, Gerd Wiebusch, Karolina Szafranska, Peter McCourt, Thomas Huser
Publicado en: Optics Express, Edición 31, 2023, ISSN 1094-4087
Editor: Optica Publishing Group
DOI: 10.1101/2023.05.11.540319

Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function (se abrirá en una nueva ventana)

Autores: Hong Mao; Larissa D. Kruse; Ruomei Li; Ana Oteiza; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Victoria C. Cogger; David Le Couteur; Deanna L. Wolfson; Thomas Huser; Balpreet Singh Ahluwalia; Cristina Øie; Peter A. G. McCourt
Publicado en: npj Gut and Liver, Edición 1, 2024, ISSN 3004-9806
Editor: Nature Publishing Group
DOI: 10.1038/s44355-024-00009-5

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy (se abrirá en una nueva ventana)

Autores: Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Image restoration in frequency space using complex-valued CNNs (se abrirá en una nueva ventana)

Autores: Zafran Hussain Shah, Marcel Müller, Wolfgang Hübner, Henning Ortkrass, Barbara Hammer, Thomas Huser, Wolfram Schenck
Publicado en: Frontiers in Artificial Intelligence, Edición 7, 2024, ISSN 2624-8212
Editor: Frontiers Media SA
DOI: 10.3389/FRAI.2024.1353873

Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2 (se abrirá en una nueva ventana)

Autores: Christopher Holte, Karolina Szafranska, Larissa Kruse, Jaione Simon-Santamaria, Ruomei Li, Dmitri Svistounov & Peter McCourt
Publicado en: Scientific Reports, Edición 13, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/S41598-023-46462-9

Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope (se abrirá en una nueva ventana)

Autores: Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Thomas Huser
Publicado en: Optics Express, Edición 31, 2023, ISSN 1094-4087
Editor: Optica Publishing Group
DOI: 10.1364/OPTICAOPEN.24033354

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