Long-term Microphysiological Sample Imaging for Evaluation of Polypharmacy in Liver

Información del proyecto

DeLIVERY

Identificador del acuerdo de subvención: 101046928

DOI

10.3030/101046928

Proyecto cerrado

Fecha de la firma de la CE 8 Marzo 2022

Fecha de inicio 1 Mayo 2022

Fecha de finalización 30 Abril 2026

Financiado con arreglo a

• The European Innovation Council (EIC)

Coste total

€ 3 547 046,00

Aportación de la UE

€ 3 547 046,00

Coordinado por

UNIVERSITAET BIELEFELD
Germany

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (11)

Sample preparation and imaging protocols in drug treatment

Pilot experiments of AFM in combination with FluidFM will be performed in order to assess the local response of LSEC to drugs and correlate dynamic changes with SIM

Report on correlation of images between AFM/FluidFM/SIM

Optical nanoscopy data will be directly overlaid by atomic force microscopy height and elastic modulus maps in software in order to assess correlative effects

Updated plan for dissemination and exploitation including communication activities

An updated plan for dissemination and exploitation including communication activities will be prepared and provided by this deliverable.

Data Management Plan

A Data Management Plan will be prepared and submitted for the entire project

Evaluation report of the near-IR sensitive sCMOS camera

This deliverable with provide a detailed report concerning the evaluation of the performance of the near-IR sensitive sCMOS camera developed within the project, incl. spectral sensitivity and read noise data.

Protocol for culture of 3D-liver samples within MPS system

This deliverable with provide a detailed protocol about the conditions required for the successful culture of 3D-liver samples within the micro physiological systems unit.

Plan for dissemination and exploitation including communication activities

A detailed issemination exploitation and communication plan will be prepared and submitted in this deliverable

Design & protocol hardware/assays of real-time LSEC porosity

This deliverable with provide a detailed report on the design and the performance of the hardware and the assays achieved with this hardware to measure liver sinusoidal endothelial cell (LSEC) porosity in realtime.

High-throughput liver function assays linked to LSEC porosity

This deliverable with provide detailed protocols for high-throughput liver function assays directly linked to real-time readouts of liver sinusoidal endothelial cell (LSEC) porosity.

RP2 update of the DMP

An update of the data management for the 2nd reporting period will be provided in this deliverable.

Protocol for culture of LSEC within the microfluidic hardware

This deliverable with provide a detailed protocol describing the culture of liver sinusoidal endothelial cells (LSEC) within the microfluidic hardware.

Websites, patent fillings, videos etc. (1)

Project website

The project website will be prepared and filled with content for public dissemination of the project its goals and its planned accomplishments

Publicaciones

Peer reviewed articles (17)

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure

Autores: Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger & Peter A. G. McCourt
Publicado en: Scientific Reports, Edición 13, 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/S41598-023-40227-0

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure

Autores: Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger, Peter A. G. McCourt
Publicado en: Scientific Reports, Edición 13, 2023, ISSN 2045-2322
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41598-023-40227-0

Frontiers in Pharmacology

Autores: Bhandari, Sabin; Karlstrøm, Ingelin Kyrrestad; Simon-Santamaria, Jaione; Li, Ruomei; Szafranska, Karolina Joanna; Dumitriu, Gianina Aurica; Sanchez Romano, Javier; Smedsrød, Bård Helge; Sørensen, Karen Kristine
Publicado en: Frontiers in Pharmacology, Edición 15, 2024, ISSN 1663-9812
Editor: Frontiers Media S.A.
DOI: 10.3389/FPHAR.2024.1377136

Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation

Autores: Dibakar Borah; Oliwia Blacharczyk; Karolina Szafranska; Izabela Czyzynska-Cichon; Sara Metwally; Konrad Szymanowski; Wolfgang Hübner; Jerzy Kotlinowski; Ewelina Dobosz; Peter McCourt; Thomas Huser; Malgorzata Lekka; Bartlomiej Zapotoczny
Publicado en: Cells, Edición 14, 2025, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/CELLS14080621

High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components

Autores: Henning Ortkrass, Jasmin Schürstedt, Gerd Wiebusch, Karolina Szafranska, Peter McCourt, Thomas Huser
Publicado en: Optics Express, Edición 31, 2023, ISSN 1094-4087
Editor: Optica Publishing Group
DOI: 10.1101/2023.05.11.540319

Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function

Autores: Hong Mao; Larissa D. Kruse; Ruomei Li; Ana Oteiza; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Victoria C. Cogger; David Le Couteur; Deanna L. Wolfson; Thomas Huser; Balpreet Singh Ahluwalia; Cristina Øie; Peter A. G. McCourt
Publicado en: npj Gut and Liver, Edición 1, 2024, ISSN 3004-9806
Editor: Nature Publishing Group
DOI: 10.1038/s44355-024-00009-5

PACA nanoparticles target and deliver sildenafil to rejuvenate aged mouse liver sinusoidal endothelial cells

Autores: Voloshyna, Tetyana; Holte, Christopher; Pospíšil, Jakub; Szafranska, Karolina; Fuskevåg, Ole Martin; Strand, Sabina P.; Åslund, Andreas K.O.; Mørch, Yrr; Snipstad, Sofie; Sulheim, Einar; Hunt, Nicholas J.; Cogger, Victoria C.; Couteur, David G. Le; Dietrichs, Erik Sveberg; McCourt, Peter A.G.
Publicado en: Nanotheranostics, Edición 9, 2025, ISSN 2206-7418
Editor: Ivyspring International Publisher
DOI: 10.7150/NTNO.103000

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy

Autores: Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Correlative Super‐Resolution Imaging of Cellular Nanopores Facilitated by Transparent Polymer Waveguide Chips

Autores: Surjendu Bikash Dutta, Jasmin Celine Schürstedt‐Seher, Anders Kokkvoll Engdahl, Wolfgang Hübner, Stefan Belle, Karolina Szafranska, Peter McCourt, Ralf Hellmann, Mark Schüttpelz, Thomas Huser
Publicado en: Advanced Optical Materials, Edición 13, 2026, ISSN 2195-1071
Editor: Wiley
DOI: 10.1002/ADOM.202402783

Hydrogen peroxide damage to rat liver sinusoidal endothelial cells is prevented by n-acetyl-cysteine but not GSH

Autores: Larissa D. Kruse; Christopher Holte; Bartlomiej Zapotoczny; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Thomas Huser; Karolina Szafranska
Publicado en: Hepatology Communications, Edición 9, 2025, ISSN 2471-254x
Editor: Lippincott
DOI: 10.1097/HC9.0000000000000617

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy

Autores: Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Image restoration in frequency space using complex-valued CNNs

Autores: Zafran Hussain Shah, Marcel Müller, Wolfgang Hübner, Henning Ortkrass, Barbara Hammer, Thomas Huser, Wolfram Schenck
Publicado en: Frontiers in Artificial Intelligence, Edición 7, 2024, ISSN 2624-8212
Editor: Frontiers Media SA
DOI: 10.3389/FRAI.2024.1353873

Super-resolution upgrade for deep tissue imaging featuring simple implementation

Autores: Patrick Byers, Thomas Kellerer, Miaomiao Li, Zhifen Chen, Thomas Huser, Thomas Hellerer
Publicado en: Nature Communications, Edición 16, 2025, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-60744-Y

Offsetting ROS-Mediated Arrest of Endothelial Fenestration Dynamics Permits Long-Term Optical Super-Resolution Imaging Validated by AFM

Autores: Annika Kiel, Marcin Luty, Angela Kralemann-Köhler, Laureen Patricia Helweg, Jasmin Schürstedt-Seher, Jerzy Kotlinowski, Jakub Pospíšil, Malgorzata Lekka, Thanh-Diep Ly, Thomas Huser, Jan Schulte Am Esch, Wolfgang Hübner, Karolina Szafranska, Bartlomiej Zapotoczny
Publicado en: ACS Applied Materials & Interfaces, 2026, ISSN 1944-8244
Editor: American Chemical Society (ACS)
DOI: 10.1021/ACSAMI.5C22333

Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

Autores: Christopher Holte, Karolina Szafranska, Larissa Kruse, Jaione Simon-Santamaria, Ruomei Li, Dmitri Svistounov, Peter McCourt
Publicado en: Scientific Reports, Edición 13, 2023, ISSN 2045-2322
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41598-023-46462-9

Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope

Autores: Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Thomas Huser
Publicado en: Optics Express, Edición 31, 2023, ISSN 1094-4087
Editor: Optica Publishing Group
DOI: 10.1364/OPTICAOPEN.24033354

Super-resolution structured illumination microscopy of cellular nanopores using near-infrared fluorescent probes

Autores: Sylvia Marie Steinecker, Henning Ortkrass, Marcel Müller, Jasmin Celine Schürstedt-Seher, Surjendu Bikash Dutta, Angela Kralemann-Köhler, Annika Kiel, Sönke Baumann, Gerhard Holst, Jan Schulte am Esch, Thomas Huser
Publicado en: Optics Express, Edición 34, 2026, ISSN 1094-4087
Editor: Optica Publishing Group
DOI: 10.1364/OE.584208