Project Results
CORDIS provides links to public deliverables and publications of HORIZON projects.
Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .
Deliverables
Pilot experiments of AFM in combination with FluidFM will be performed in order to assess the local response of LSEC to drugs and correlate dynamic changes with SIM
Report on correlation of images between AFM/FluidFM/SIM (opens in new window)Optical nanoscopy data will be directly overlaid by atomic force microscopy height and elastic modulus maps in software in order to assess correlative effects
Ex vivo drug testing of LSEC morphology and liver function (opens in new window)This deliverable will provide detailed protocols for how ex vivo drug testing of liver sinusoidal endothelial cells (LSEC) morphology and liver function was achieved and which performance parameters were obtained.
Updated plan for dissemination and exploitation including communication activities (opens in new window)An updated plan for dissemination and exploitation including communication activities will be prepared and provided by this deliverable.
Efficacy of new/existing pharma agents in restoring LSEC (opens in new window)This deliverable will provide a detailed report on the efficacy of new and existing pharmacological agents, such as sildenafil and its analogues as well as anti-aging drugs in enhancing and/or restoring the morphology and function of liver sinusoidal endothelial cells (LSEC).
Data Management Plan (opens in new window)A Data Management Plan will be prepared and submitted for the entire project
Final update of the DMP (opens in new window)An update of the data management plan will be provided for the final reporting period of the project.
Final dissemination and exploitation plan (opens in new window)A final updated dissemination and exploitation plan will be prepared and provided by this deliverable.
Drug library screening linked to LSEC morphology/liver function (opens in new window)This deliverable will provide detailed protocols for how drug library screening of LSEC morphology and liver function was accomplished and how it is linked to these parameters.
Evaluation report of the near-IR sensitive sCMOS camera (opens in new window)This deliverable with provide a detailed report concerning the evaluation of the performance of the near-IR sensitive sCMOS camera developed within the project, incl. spectral sensitivity and read noise data.
Compatibility of refenestration and anti-fibrotic therapies (opens in new window)This deliverable will provide a detailed report on the compatibility of novel refenestration and anti-fibrotic therapies (e.g. sildenafil application and the use of sildenafil analogues) within poly-pharmacy contexts, i.e. by testing against cross-reactions with other common drugs, such as pain killers, blood thinners, etc.
High-throughput ex-vivo/in vitro testing for polypharmacy (opens in new window)This deliverable will provide detailed protocols for how high-throughput ex-vivo/in vitro testing for effects of polypharmacy challenge of liver cells was accomplished and which performance parameters were achieved.
Multiparametric testing of at least 4 agents (opens in new window)The response of perfused liver samples to selected drugs will be assessed by atomic force microscopy with FluidFM, a method to locally expose cells to drugs
Protocol for culture of 3D-liver samples within MPS system (opens in new window)This deliverable with provide a detailed protocol about the conditions required for the successful culture of 3D-liver samples within the micro physiological systems unit.
Report on integration of DL methods (opens in new window)This deliverable will provide a detailed report about the integration of deep learning (DL) methods in controlling the microphysiological systems unit as well as their application to image processing and analysis.
Plan for dissemination and exploitation including communication activities (opens in new window)A detailed issemination exploitation and communication plan will be prepared and submitted in this deliverable
Design & protocol hardware/assays of real-time LSEC porosity (opens in new window)This deliverable with provide a detailed report on the design and the performance of the hardware and the assays achieved with this hardware to measure liver sinusoidal endothelial cell (LSEC) porosity in realtime.
High-throughput liver function assays linked to LSEC porosity (opens in new window)This deliverable with provide detailed protocols for high-throughput liver function assays directly linked to real-time readouts of liver sinusoidal endothelial cell (LSEC) porosity.
RP2 update of the DMP (opens in new window)An update of the data management for the 2nd reporting period will be provided in this deliverable.
Protocol for culture of LSEC within the microfluidic hardware (opens in new window)This deliverable with provide a detailed protocol describing the culture of liver sinusoidal endothelial cells (LSEC) within the microfluidic hardware.
Future roadmap for technological development and further funding (opens in new window)A roadmap detailing the future technological development activities and funding strategies planned by the project participants will be prepared and provided by this deliverable.
This deliverable will provide details about the proof of principle of the micro physiological systems unit and how this unit combined with novel, high performance optical readout methods was utilized in the screening of novel drugs to improve LSEC function.
Visualization of LSEC fenestrations in liver tissue (opens in new window)The local elastic modulus of liver tissue samples will be mapped and evaluated by atomic force microscopy and overlaid with super-resolution structured illumination microscopy data
The project website will be prepared and filled with content for public dissemination of the project its goals and its planned accomplishments
Publications
Author(s):
Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger & Peter A. G. McCourt
Published in:
Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher:
Nature Publishing Group
DOI:
10.1038/S41598-023-40227-0
Author(s):
Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger, Peter A. G. McCourt
Published in:
Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher:
Springer Science and Business Media LLC
DOI:
10.1038/S41598-023-40227-0
Author(s):
Bhandari, Sabin; Karlstrøm, Ingelin Kyrrestad; Simon-Santamaria, Jaione; Li, Ruomei; Szafranska, Karolina Joanna; Dumitriu, Gianina Aurica; Sanchez Romano, Javier; Smedsrød, Bård Helge; Sørensen, Karen Kristine
Published in:
Frontiers in Pharmacology, Issue 15, 2024, ISSN 1663-9812
Publisher:
Frontiers Media S.A.
DOI:
10.3389/FPHAR.2024.1377136
Author(s):
Dibakar Borah; Oliwia Blacharczyk; Karolina Szafranska; Izabela Czyzynska-Cichon; Sara Metwally; Konrad Szymanowski; Wolfgang Hübner; Jerzy Kotlinowski; Ewelina Dobosz; Peter McCourt; Thomas Huser; Malgorzata Lekka; Bartlomiej Zapotoczny
Published in:
Cells, Issue 14, 2025, ISSN 2073-4409
Publisher:
MDPI
DOI:
10.3390/CELLS14080621
Author(s):
Henning Ortkrass, Jasmin Schürstedt, Gerd Wiebusch, Karolina Szafranska, Peter McCourt, Thomas Huser
Published in:
Optics Express, Issue 31, 2023, ISSN 1094-4087
Publisher:
Optica Publishing Group
DOI:
10.1101/2023.05.11.540319
Author(s):
Hong Mao; Larissa D. Kruse; Ruomei Li; Ana Oteiza; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Victoria C. Cogger; David Le Couteur; Deanna L. Wolfson; Thomas Huser; Balpreet Singh Ahluwalia; Cristina Øie; Peter A. G. McCourt
Published in:
npj Gut and Liver, Issue 1, 2024, ISSN 3004-9806
Publisher:
Nature Publishing Group
DOI:
10.1038/s44355-024-00009-5
Author(s):
Voloshyna, Tetyana; Holte, Christopher; Pospíšil, Jakub; Szafranska, Karolina; Fuskevåg, Ole Martin; Strand, Sabina P.; Åslund, Andreas K.O.; Mørch, Yrr; Snipstad, Sofie; Sulheim, Einar; Hunt, Nicholas J.; Cogger, Victoria C.; Couteur, David G. Le; Dietrichs, Erik Sveberg; McCourt, Peter A.G.
Published in:
Nanotheranostics, Issue 9, 2025, ISSN 2206-7418
Publisher:
Ivyspring International Publisher
DOI:
10.7150/NTNO.103000
Author(s):
Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Published in:
Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher:
Springer Science and Business Media LLC
DOI:
10.1038/S41467-024-53198-1
Author(s):
Surjendu Bikash Dutta, Jasmin Celine Schürstedt‐Seher, Anders Kokkvoll Engdahl, Wolfgang Hübner, Stefan Belle, Karolina Szafranska, Peter McCourt, Ralf Hellmann, Mark Schüttpelz, Thomas Huser
Published in:
Advanced Optical Materials, Issue 13, 2026, ISSN 2195-1071
Publisher:
Wiley
DOI:
10.1002/ADOM.202402783
Author(s):
Larissa D. Kruse; Christopher Holte; Bartlomiej Zapotoczny; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Thomas Huser; Karolina Szafranska
Published in:
Hepatology Communications, Issue 9, 2025, ISSN 2471-254x
Publisher:
Lippincott
DOI:
10.1097/HC9.0000000000000617
Author(s):
Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Published in:
Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher:
Springer Science and Business Media LLC
DOI:
10.1038/S41467-024-53198-1
Author(s):
Zafran Hussain Shah, Marcel Müller, Wolfgang Hübner, Henning Ortkrass, Barbara Hammer, Thomas Huser, Wolfram Schenck
Published in:
Frontiers in Artificial Intelligence, Issue 7, 2024, ISSN 2624-8212
Publisher:
Frontiers Media SA
DOI:
10.3389/FRAI.2024.1353873
Author(s):
Patrick Byers, Thomas Kellerer, Miaomiao Li, Zhifen Chen, Thomas Huser, Thomas Hellerer
Published in:
Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher:
Springer Science and Business Media LLC
DOI:
10.1038/S41467-025-60744-Y
Author(s):
Annika Kiel, Marcin Luty, Angela Kralemann-Köhler, Laureen Patricia Helweg, Jasmin Schürstedt-Seher, Jerzy Kotlinowski, Jakub Pospíšil, Malgorzata Lekka, Thanh-Diep Ly, Thomas Huser, Jan Schulte Am Esch, Wolfgang Hübner, Karolina Szafranska, Bartlomiej Zapotoczny
Published in:
ACS Applied Materials & Interfaces, 2026, ISSN 1944-8244
Publisher:
American Chemical Society (ACS)
DOI:
10.1021/ACSAMI.5C22333
Author(s):
Christopher Holte, Karolina Szafranska, Larissa Kruse, Jaione Simon-Santamaria, Ruomei Li, Dmitri Svistounov, Peter McCourt
Published in:
Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher:
Springer Science and Business Media LLC
DOI:
10.1038/S41598-023-46462-9
Author(s):
Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Thomas Huser
Published in:
Optics Express, Issue 31, 2023, ISSN 1094-4087
Publisher:
Optica Publishing Group
DOI:
10.1364/OPTICAOPEN.24033354
Author(s):
Sylvia Marie Steinecker, Henning Ortkrass, Marcel Müller, Jasmin Celine Schürstedt-Seher, Surjendu Bikash Dutta, Angela Kralemann-Köhler, Annika Kiel, Sönke Baumann, Gerhard Holst, Jan Schulte am Esch, Thomas Huser
Published in:
Optics Express, Issue 34, 2026, ISSN 1094-4087
Publisher:
Optica Publishing Group
DOI:
10.1364/OE.584208
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