Long-term Microphysiological Sample Imaging for Evaluation of Polypharmacy in Liver

Project Information

DeLIVERY

Grant agreement ID: 101046928

DOI

10.3030/101046928

EC signature date 8 March 2022

Start date 1 May 2022

End date 30 April 2026

Funded under

• The European Innovation Council (EIC)

Total cost

€ 3 547 046,50

EU contribution

€ 3 547 046,00

Coordinated by

UNIVERSITAET BIELEFELD
Germany

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Deliverables

Documents, reports (11)

Sample preparation and imaging protocols in drug treatment

Pilot experiments of AFM in combination with FluidFM will be performed in order to assess the local response of LSEC to drugs and correlate dynamic changes with SIM

Report on correlation of images between AFM/FluidFM/SIM

Optical nanoscopy data will be directly overlaid by atomic force microscopy height and elastic modulus maps in software in order to assess correlative effects

Updated plan for dissemination and exploitation including communication activities

An updated plan for dissemination and exploitation including communication activities will be prepared and provided by this deliverable.

Data Management Plan

A Data Management Plan will be prepared and submitted for the entire project

Evaluation report of the near-IR sensitive sCMOS camera

This deliverable with provide a detailed report concerning the evaluation of the performance of the near-IR sensitive sCMOS camera developed within the project, incl. spectral sensitivity and read noise data.

Protocol for culture of 3D-liver samples within MPS system

This deliverable with provide a detailed protocol about the conditions required for the successful culture of 3D-liver samples within the micro physiological systems unit.

Plan for dissemination and exploitation including communication activities

A detailed issemination exploitation and communication plan will be prepared and submitted in this deliverable

Design & protocol hardware/assays of real-time LSEC porosity

This deliverable with provide a detailed report on the design and the performance of the hardware and the assays achieved with this hardware to measure liver sinusoidal endothelial cell (LSEC) porosity in realtime.

High-throughput liver function assays linked to LSEC porosity

This deliverable with provide detailed protocols for high-throughput liver function assays directly linked to real-time readouts of liver sinusoidal endothelial cell (LSEC) porosity.

RP2 update of the DMP

An update of the data management for the 2nd reporting period will be provided in this deliverable.

Protocol for culture of LSEC within the microfluidic hardware

This deliverable with provide a detailed protocol describing the culture of liver sinusoidal endothelial cells (LSEC) within the microfluidic hardware.

Websites, patent fillings, videos etc. (1)

Project website

The project website will be prepared and filled with content for public dissemination of the project its goals and its planned accomplishments

Publications

Peer reviewed articles (7)

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure

Author(s): Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger & Peter A. G. McCourt
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/S41598-023-40227-0

High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components

Author(s): Henning Ortkrass, Jasmin Schürstedt, Gerd Wiebusch, Karolina Szafranska, Peter McCourt, Thomas Huser
Published in: Optics Express, Issue 31, 2023, ISSN 1094-4087
Publisher: Optica Publishing Group
DOI: 10.1101/2023.05.11.540319

Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function

Author(s): Hong Mao; Larissa D. Kruse; Ruomei Li; Ana Oteiza; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Victoria C. Cogger; David Le Couteur; Deanna L. Wolfson; Thomas Huser; Balpreet Singh Ahluwalia; Cristina Øie; Peter A. G. McCourt
Published in: npj Gut and Liver, Issue 1, 2024, ISSN 3004-9806
Publisher: Nature Publishing Group
DOI: 10.1038/s44355-024-00009-5

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy

Author(s): Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Image restoration in frequency space using complex-valued CNNs

Author(s): Zafran Hussain Shah, Marcel Müller, Wolfgang Hübner, Henning Ortkrass, Barbara Hammer, Thomas Huser, Wolfram Schenck
Published in: Frontiers in Artificial Intelligence, Issue 7, 2024, ISSN 2624-8212
Publisher: Frontiers Media SA
DOI: 10.3389/FRAI.2024.1353873

Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

Author(s): Christopher Holte, Karolina Szafranska, Larissa Kruse, Jaione Simon-Santamaria, Ruomei Li, Dmitri Svistounov & Peter McCourt
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/S41598-023-46462-9

Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope

Author(s): Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Thomas Huser
Published in: Optics Express, Issue 31, 2023, ISSN 1094-4087
Publisher: Optica Publishing Group
DOI: 10.1364/OPTICAOPEN.24033354