Long-term Microphysiological Sample Imaging for Evaluation of Polypharmacy in Liver

Deliverables

Sample preparation and imaging protocols in drug treatment

Pilot experiments of AFM in combination with FluidFM will be performed in order to assess the local response of LSEC to drugs and correlate dynamic changes with SIM

Report on correlation of images between AFM/FluidFM/SIM

Optical nanoscopy data will be directly overlaid by atomic force microscopy height and elastic modulus maps in software in order to assess correlative effects

Updated plan for dissemination and exploitation including communication activities

An updated plan for dissemination and exploitation including communication activities will be prepared and provided by this deliverable.

Data Management Plan

A Data Management Plan will be prepared and submitted for the entire project

Evaluation report of the near-IR sensitive sCMOS camera

This deliverable with provide a detailed report concerning the evaluation of the performance of the near-IR sensitive sCMOS camera developed within the project, incl. spectral sensitivity and read noise data.

Protocol for culture of 3D-liver samples within MPS system

This deliverable with provide a detailed protocol about the conditions required for the successful culture of 3D-liver samples within the micro physiological systems unit.

Plan for dissemination and exploitation including communication activities

A detailed issemination exploitation and communication plan will be prepared and submitted in this deliverable

Design & protocol hardware/assays of real-time LSEC porosity

This deliverable with provide a detailed report on the design and the performance of the hardware and the assays achieved with this hardware to measure liver sinusoidal endothelial cell (LSEC) porosity in realtime.

High-throughput liver function assays linked to LSEC porosity

This deliverable with provide detailed protocols for high-throughput liver function assays directly linked to real-time readouts of liver sinusoidal endothelial cell (LSEC) porosity.

RP2 update of the DMP

An update of the data management for the 2nd reporting period will be provided in this deliverable.

Protocol for culture of LSEC within the microfluidic hardware

This deliverable with provide a detailed protocol describing the culture of liver sinusoidal endothelial cells (LSEC) within the microfluidic hardware.

Project website

The project website will be prepared and filled with content for public dissemination of the project its goals and its planned accomplishments

Publications

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure

Author(s): Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger & Peter A. G. McCourt
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/S41598-023-40227-0

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure

Author(s): Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger, Peter A. G. McCourt
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41598-023-40227-0

Frontiers in Pharmacology

Author(s): Bhandari, Sabin; Karlstrøm, Ingelin Kyrrestad; Simon-Santamaria, Jaione; Li, Ruomei; Szafranska, Karolina Joanna; Dumitriu, Gianina Aurica; Sanchez Romano, Javier; Smedsrød, Bård Helge; Sørensen, Karen Kristine
Published in: Frontiers in Pharmacology, Issue 15, 2024, ISSN 1663-9812
Publisher: Frontiers Media S.A.
DOI: 10.3389/FPHAR.2024.1377136

Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation

Author(s): Dibakar Borah; Oliwia Blacharczyk; Karolina Szafranska; Izabela Czyzynska-Cichon; Sara Metwally; Konrad Szymanowski; Wolfgang Hübner; Jerzy Kotlinowski; Ewelina Dobosz; Peter McCourt; Thomas Huser; Malgorzata Lekka; Bartlomiej Zapotoczny
Published in: Cells, Issue 14, 2025, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/CELLS14080621

High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components

Author(s): Henning Ortkrass, Jasmin Schürstedt, Gerd Wiebusch, Karolina Szafranska, Peter McCourt, Thomas Huser
Published in: Optics Express, Issue 31, 2023, ISSN 1094-4087
Publisher: Optica Publishing Group
DOI: 10.1101/2023.05.11.540319

Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function

Author(s): Hong Mao; Larissa D. Kruse; Ruomei Li; Ana Oteiza; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Victoria C. Cogger; David Le Couteur; Deanna L. Wolfson; Thomas Huser; Balpreet Singh Ahluwalia; Cristina Øie; Peter A. G. McCourt
Published in: npj Gut and Liver, Issue 1, 2024, ISSN 3004-9806
Publisher: Nature Publishing Group
DOI: 10.1038/s44355-024-00009-5

PACA nanoparticles target and deliver sildenafil to rejuvenate aged mouse liver sinusoidal endothelial cells

Author(s): Voloshyna, Tetyana; Holte, Christopher; Pospíšil, Jakub; Szafranska, Karolina; Fuskevåg, Ole Martin; Strand, Sabina P.; Åslund, Andreas K.O.; Mørch, Yrr; Snipstad, Sofie; Sulheim, Einar; Hunt, Nicholas J.; Cogger, Victoria C.; Couteur, David G. Le; Dietrichs, Erik Sveberg; McCourt, Peter A.G.
Published in: Nanotheranostics, Issue 9, 2025, ISSN 2206-7418
Publisher: Ivyspring International Publisher
DOI: 10.7150/NTNO.103000

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy

Author(s): Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Correlative Super‐Resolution Imaging of Cellular Nanopores Facilitated by Transparent Polymer Waveguide Chips

Author(s): Surjendu Bikash Dutta, Jasmin Celine Schürstedt‐Seher, Anders Kokkvoll Engdahl, Wolfgang Hübner, Stefan Belle, Karolina Szafranska, Peter McCourt, Ralf Hellmann, Mark Schüttpelz, Thomas Huser
Published in: Advanced Optical Materials, Issue 13, 2026, ISSN 2195-1071
Publisher: Wiley
DOI: 10.1002/ADOM.202402783

Hydrogen peroxide damage to rat liver sinusoidal endothelial cells is prevented by n-acetyl-cysteine but not GSH

Author(s): Larissa D. Kruse; Christopher Holte; Bartlomiej Zapotoczny; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Thomas Huser; Karolina Szafranska
Published in: Hepatology Communications, Issue 9, 2025, ISSN 2471-254x
Publisher: Lippincott
DOI: 10.1097/HC9.0000000000000617

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy

Author(s): Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Image restoration in frequency space using complex-valued CNNs

Author(s): Zafran Hussain Shah, Marcel Müller, Wolfgang Hübner, Henning Ortkrass, Barbara Hammer, Thomas Huser, Wolfram Schenck
Published in: Frontiers in Artificial Intelligence, Issue 7, 2024, ISSN 2624-8212
Publisher: Frontiers Media SA
DOI: 10.3389/FRAI.2024.1353873

Super-resolution upgrade for deep tissue imaging featuring simple implementation

Author(s): Patrick Byers, Thomas Kellerer, Miaomiao Li, Zhifen Chen, Thomas Huser, Thomas Hellerer
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-60744-Y

Offsetting ROS-Mediated Arrest of Endothelial Fenestration Dynamics Permits Long-Term Optical Super-Resolution Imaging Validated by AFM

Author(s): Annika Kiel, Marcin Luty, Angela Kralemann-Köhler, Laureen Patricia Helweg, Jasmin Schürstedt-Seher, Jerzy Kotlinowski, Jakub Pospíšil, Malgorzata Lekka, Thanh-Diep Ly, Thomas Huser, Jan Schulte Am Esch, Wolfgang Hübner, Karolina Szafranska, Bartlomiej Zapotoczny
Published in: ACS Applied Materials & Interfaces, 2026, ISSN 1944-8244
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSAMI.5C22333

Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

Author(s): Christopher Holte, Karolina Szafranska, Larissa Kruse, Jaione Simon-Santamaria, Ruomei Li, Dmitri Svistounov, Peter McCourt
Published in: Scientific Reports, Issue 13, 2023, ISSN 2045-2322
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41598-023-46462-9

Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope

Author(s): Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Thomas Huser
Published in: Optics Express, Issue 31, 2023, ISSN 1094-4087
Publisher: Optica Publishing Group
DOI: 10.1364/OPTICAOPEN.24033354

Super-resolution structured illumination microscopy of cellular nanopores using near-infrared fluorescent probes

Author(s): Sylvia Marie Steinecker, Henning Ortkrass, Marcel Müller, Jasmin Celine Schürstedt-Seher, Surjendu Bikash Dutta, Angela Kralemann-Köhler, Annika Kiel, Sönke Baumann, Gerhard Holst, Jan Schulte am Esch, Thomas Huser
Published in: Optics Express, Issue 34, 2026, ISSN 1094-4087
Publisher: Optica Publishing Group
DOI: 10.1364/OE.584208

Deliverables

Publications

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