Long-term Microphysiological Sample Imaging for Evaluation of Polypharmacy in Liver

Informations projet

DeLIVERY

N° de convention de subvention: 101046928

DOI

10.3030/101046928

Date de signature de la CE 8 Mars 2022

Date de début 1 Mai 2022

Date de fin 30 Avril 2026

Financé au titre de

• The European Innovation Council (EIC)

Coût total

€ 3 547 046,50

Contribution de l’UE

€ 3 547 046,00

Coordonné par

UNIVERSITAET BIELEFELD
Germany

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (11)

Sample preparation and imaging protocols in drug treatment

Pilot experiments of AFM in combination with FluidFM will be performed in order to assess the local response of LSEC to drugs and correlate dynamic changes with SIM

Report on correlation of images between AFM/FluidFM/SIM

Optical nanoscopy data will be directly overlaid by atomic force microscopy height and elastic modulus maps in software in order to assess correlative effects

Updated plan for dissemination and exploitation including communication activities

An updated plan for dissemination and exploitation including communication activities will be prepared and provided by this deliverable.

Data Management Plan

A Data Management Plan will be prepared and submitted for the entire project

Evaluation report of the near-IR sensitive sCMOS camera

This deliverable with provide a detailed report concerning the evaluation of the performance of the near-IR sensitive sCMOS camera developed within the project, incl. spectral sensitivity and read noise data.

Protocol for culture of 3D-liver samples within MPS system

This deliverable with provide a detailed protocol about the conditions required for the successful culture of 3D-liver samples within the micro physiological systems unit.

Plan for dissemination and exploitation including communication activities

A detailed issemination exploitation and communication plan will be prepared and submitted in this deliverable

Design & protocol hardware/assays of real-time LSEC porosity

This deliverable with provide a detailed report on the design and the performance of the hardware and the assays achieved with this hardware to measure liver sinusoidal endothelial cell (LSEC) porosity in realtime.

High-throughput liver function assays linked to LSEC porosity

This deliverable with provide detailed protocols for high-throughput liver function assays directly linked to real-time readouts of liver sinusoidal endothelial cell (LSEC) porosity.

RP2 update of the DMP

An update of the data management for the 2nd reporting period will be provided in this deliverable.

Protocol for culture of LSEC within the microfluidic hardware

This deliverable with provide a detailed protocol describing the culture of liver sinusoidal endothelial cells (LSEC) within the microfluidic hardware.

Websites, patent fillings, videos etc. (1)

Project website

The project website will be prepared and filled with content for public dissemination of the project its goals and its planned accomplishments

Publications

Peer reviewed articles (7)

Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure

Auteurs: Hong Mao, Karolina Szafranska, Larissa Kruse, Christopher Holte, Deanna L. Wolfson, Balpreet Singh Ahluwalia, Cynthia B. Whitchurch, Louise Cole, Glen P. Lockwood, Robin Diekmann, David Le Couteur, Victoria C. Cogger & Peter A. G. McCourt
Publié dans: Scientific Reports, Numéro 13, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/S41598-023-40227-0

High-speed TIRF and 2D super-resolution structured illumination microscopy with a large field of view based on fiber optic components

Auteurs: Henning Ortkrass, Jasmin Schürstedt, Gerd Wiebusch, Karolina Szafranska, Peter McCourt, Thomas Huser
Publié dans: Optics Express, Numéro 31, 2023, ISSN 1094-4087
Éditeur: Optica Publishing Group
DOI: 10.1101/2023.05.11.540319

Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function

Auteurs: Hong Mao; Larissa D. Kruse; Ruomei Li; Ana Oteiza; Eike C. Struck; Jasmin Schürstedt; Wolfgang Hübner; Victoria C. Cogger; David Le Couteur; Deanna L. Wolfson; Thomas Huser; Balpreet Singh Ahluwalia; Cristina Øie; Peter A. G. McCourt
Publié dans: npj Gut and Liver, Numéro 1, 2024, ISSN 3004-9806
Éditeur: Nature Publishing Group
DOI: 10.1038/s44355-024-00009-5

High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy

Auteurs: Henning Ortkrass, Marcel Müller, Anders Kokkvoll Engdahl, Gerhard Holst, Thomas Huser
Publié dans: Nature Communications, Numéro 15, 2024, ISSN 2041-1723
Éditeur: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-53198-1

Image restoration in frequency space using complex-valued CNNs

Auteurs: Zafran Hussain Shah, Marcel Müller, Wolfgang Hübner, Henning Ortkrass, Barbara Hammer, Thomas Huser, Wolfram Schenck
Publié dans: Frontiers in Artificial Intelligence, Numéro 7, 2024, ISSN 2624-8212
Éditeur: Frontiers Media SA
DOI: 10.3389/FRAI.2024.1353873

Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

Auteurs: Christopher Holte, Karolina Szafranska, Larissa Kruse, Jaione Simon-Santamaria, Ruomei Li, Dmitri Svistounov & Peter McCourt
Publié dans: Scientific Reports, Numéro 13, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/S41598-023-46462-9

Grazing incidence to total internal reflection fluorescence structured illumination microscopy enabled by a prism telescope

Auteurs: Henning Ortkrass, Gerd Wiebusch, Jochen Linnenbrügger, Jasmin Schürstedt, Karolina Szafranska, Peter McCourt, Thomas Huser
Publié dans: Optics Express, Numéro 31, 2023, ISSN 1094-4087
Éditeur: Optica Publishing Group
DOI: 10.1364/OPTICAOPEN.24033354