CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.
I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .
Risultati finali
My project will identify and characterize transcription regulators involved in primary cell wall metabolism and molecular components regulating SA production. Multiple methods will be used in this project. (Phospho)proteomics and transcriptomics data will be analyzed to generate a list of SA signal transduction candidate genes and transcription regulators crucial in response to ISX. Around 20-40 T-DNA insertion lines for candidate genes will be ordered from the NASC stock center. Phenotypic characterization of the insertion lines will be performed to identify candidates where the loss of function affects SA production and cell wall metabolism changes induced by CWI impairment in Arabidopsis seedlings. Maximal four candidates that are exhibiting the most substantial (loss of function) phenotypes will be selected for follow-up studies. Functional characterizations of candidates will be performed, including localization, expression/co-immunoprecipitation studies, and characterization of mutant phenotypes. In addition, identifying those Arabidopsis candidates affect performance-relevant stress traits homologs in commercially relevant food and bioenergy crops. The experiments planned here will generate different types of data. The data generated from this project will be in the form of: (1) Digital images, (confocal laser scanning microscopy (CLSM) and widefield microscope), and photographs showing phenotypes of transgenic plants. Confocal data will be stored as files containing all the raw spatial/quantitative information needed for any subsequent analysis. Processed data displays will be compiled in summary presentations. (2) Results from phenotypic analyses of all samples will be generated. These data will be compiled in an openly accessible format, and raw data will be stored on a data repository server maintained by NTNU. (3) qRT-PCR data generated with the available qRT-PCR systems will be stored in Excel file format. (4) Data generated in proteomics/transcriptomics experiments will be stored both in original raw file formats and in standardized lists in excel format. (5) Data from the research will be detailed in publications, with the use of web-based supplementary data displays where possible, and the available access to our data repository. All manuscripts will be published in open access format to facilitate accessibility. Any novel data regarding the functions of the genes characterized will be deposited in the Arabidopsis database. IP issues will be pursued as required in order to safeguard public access to any tools developed during this project. This will be done in consultation with the NTNU Spin out consultants. To reach the non-academic community, I will use internet-based tools such as a Blog, Twitter, and a project website to share significant progress in my project.
Communication, Dissemination and Exploitation Plan (si apre in una nuova finestra)"I will write and publish a review in open-access format summarizing current knowledge about CWI maintenance, transcriptional regulation, and SA-mediated processes in plants, targeted at audiences reading general natural sciences, high-impact, peer-reviewed journals. I will share the knowledge generated in my project with the scientific community through at least one publication in a plant science-related journal in an open-access format. Publishing these articles will help me to establish myself in the plant cell wall research community. In addition, I will attend at least two relevant international scientific conferences, such as the International Conference on Arabidopsis Research (ICAR; Makuhari Messe, Chiba, Japan, June 5 - 9, 2023) and the next plant cell wall conference (Cell Wall Meeting in Malaga, Spain, in 2023). I will also communicate with the public via social networks using a project-specific Twitter account, ResearchGate, and LinkedIn profiles to inform about project progress and exciting new findings to increase the impact of my publications. Social networking will also ensure that the research conducted in this project will have the highest possible visibility to reach relevant audiences in the scientific (i.e. potential collaborators) and industrial communities (i.e. companies interested in exploiting the results) as well as the public. Efforts to improve biomass yield and biofuel production in bioenergy crops by modifying plant cell walls are excellent examples of how novel knowledge may be exploited. Many of these efforts have not been successful due to the plasticity of the cell walls. Both development and immunity affect food crop performance. Therefore, results from this project can create the knowledge base to pursue new strategies to improve food crop performance and facilitate bioenergy production in the long term. One consequence of this is that valorization opportunities may arise during this project, specifically if we find any gene of interest modulating CWI in response to growth and/or defense that can be used as leads to improve the performance of crop plants.I will work with the NTNU technology transfer office to secure Intellectual Property Rights protection and, if sensible, start patenting procedures. Moreover, my host group has connections to Graminor AS, a Norwegian breeding company, and Plant Response Biotech SL, founded by a close collaborator from the Technical University of Madrid (Prof. Antonio Molina Fernández). I will try to use these connections to assess the valorization potential of my research outputs. In parallel, I plan to use my family's connections in the Czech Republic to interact with agricultural networks (farmers) to attract grassroots attention to newly developed methods that facilitate sustainable food and energy production. NTNU is encouraging researchers to participate in outreach activities. One of them is Researcher's Night, where pupils from local high schools visit research groups at NTNU. The second is the ""forskningsdagene"" (research days), representing a national science festival showcasing ongoing activities. In addition, my host group is inviting pupils from high schools to undertake lab rotations to get exposure to basic science. I plan to participate in these events to enhance the visibility of EU-funded research and improve public understanding of science."
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