Related to WP1, we have devised several kinds of new magnets, by improving magnet tip designs, in order to generate a range of devices that can be used for various range of force application. In addition, we implemented a systematic experimental protocol and home-made image analysis pipeline to calibrate the magnetic force. The experimental assay is based on tracking the velocity of magnetic beads in test viscous solution (Glycerol 80%), as a function of their size and distance to the magnet tip. Related to WP2, we performed novel assays to use the device to other biological contexts. Notably we: (i) performed experiments in drosophila embryo (collaboration with Y. Bellaïche), and demonstrated that the device can be used to manipulate spindle position in large developing embryos, as well as apply forces on intercellular junctions in order to minor their mechanical properties, as well as assays mechanosensing pathways at junctions. (ii) We used the device to perform a systematic mapping of viscoelastic properties of bulk cytoplasm during the development of sea urchin embryos. (iii) Implemented a novel assay based on the use of water in oil droplets that encapsulate cytoplasm extracts and large magnetic probe to preform rheological measurements in a medium-throughput manner in different kind of cytoplasm extracts. Importantly throughout these novel assays in different living multicellular systems and in vitro, we improved methods for injecting and coating magnetic probes, which also helps contribute to the execution of WP1 and WP3. As a continuation of the project, we are currently collaborating with 2 other groups to implement and test the device in the context of multicellular intestinal organoids (Collaboration, D. Delacour, IBDM, Marseille, France) and the regeneration of heart tissues in zebrafish embryos (Collaboration with Kimara Targoff, Columbia University, NY, USA). Related to WP3, we have initiated to define the content of kits with out TTO partner CNRS innovation. The kits shall be ready to be shared in the next coming months, as the material is readily available. Related to WP4, our TTO partner CNRS innovation has (i) conducted an anylsis of the technology with respect to the state of the art, (ii) perfomed an interview of KOLs; (iii) expanded the IP by filing european and US extension of the patent and (iv) initiated discussions with our prefered industrial partner Impetux.
