Highly multiplexed, quantitative protein biomarker profiling (hmqPro)

Projektinformationen

hmqPro

ID Finanzhilfevereinbarung: 101069454

DOI

10.3030/101069454

Projekt abgeschlossen

EK-Unterschriftsdatum 16 Mai 2022

Startdatum 1 Juli 2022

Enddatum 31 Dezember 2023

Finanziert unter

• European Research Council (ERC)

Gesamtkosten

Keine Daten

EU-Beitrag

€ 150 000,00

Koordiniert durch

KAROLINSKA INSTITUTET
Sweden

Periodic Reporting for period 1 - hmqPro (Highly multiplexed, quantitative protein biomarker profiling (hmqPro))

Berichtszeitraum: 2022-07-01 bis 2023-12-31

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

The project will develop a multipurpose, highly multiplexed, quantitative protein profiling (hmqPro) platform that can be adapted to a wide range of applications, particularly biomarker discovery. Biomarkers are a 43 Billion USD market spanning applications in diagnostics, drug discovery & development, personalized medicine, disease risk assessment. The project aimed to develop a new quantitative protein profiling platform without the need for specialized laboratory equipment that will have the capacity to impact the biomarker discovery market by combining unique flexibility with high-throughput.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

In an academic-commercial partnership between Karolinska Institutet and Epigenica AB, technological foundations were laid for a method comprising antibody-based quantitation of multiple proteins in a large number of samples. Various chemistries and molecular biology steps in the procedure required extensive optimization and trouble-shooting. Alternative quantitation strategies on solid support and in solution were piloted. The outcome of the project is a optimized reagents design and workflow that we will further develop into the intended highly multiplexed protein profiling method in the future.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

In parallel with validating the fundamentals of the intended assay platform, we have performed market analysis focussing specifically on diagnostic applications of protein profiling methods in liquid biopsies.
Following up on the results and insights gained in this project, we will continue the product development trajectory, first completing development of the assay platform, then performing a variety of validations on different biological source material and protein targets. Ultimately, the novel hmqPRO assay platform will join Epigenica AB's product development program in form of kit and service offerings.