Project description DEENESFRITPL Real-time DNA recognition mapping developed Surveillance of various diseases like cancer can be time consuming and expensive. Securing a faster, less- invasive method for monitoring them would be advantageous. The EU-funded BIoRead project has set its sights on developing nano-devices to analyse DNA single molecules for liquid biopsy. ‘Laser-Assisted DNA Optical Mapping’ (LADOM) is the result — a method that can detect DNA molecules from a sample in real time. LADOM yields quick and simple DNA analysis and detects tiny DNA fragments with less than a microlitre of fluid used. By leveraging this high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications, the project aims to standardise the technology and bring it to market. Show the project objective Hide the project objective Objective The aim of this project is to use an ultra-fast, high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications. The proposed methodology, “Laser-Assisted DNA Optical Mapping (LADOM)”, allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device. It combines a cheap device fabrication, flexible DNA labelling (customizable for different applications), microscope- and camera-free set up, a read-out sensitive to single molecules, very high throughput (tens of molecules per minute), ability to detect very small fragments, and no limitation for the maximum molecule’s length, what is especially interesting for applications dealing with ultra-long intact, genomic DNA. In this project, we request funding to offer a service to analyze samples from possible stakeholders from the biomedical sector, and to standardize the technology and improve the troughput bThe aim of this project is to use an ultra-fast, high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications. The proposed methodology, “Laser-Assisted DNA Optical Mapping (LADOM)”, allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device. It combines a cheap device fabrication, flexible DNA labelling (customizable for different applications), microscope- and camera-free set up, a read-out sensitive to single molecules, very high throughput (tens of molecules per minute), ability to detect very small fragments, and no limitation for the maximum molecule’s length, what is especially interesting for applications dealing with ultra-long intact, genomic DNA. In this project, we request funding to offer a service to analyze samples from possible stakeholders from the biomedical sector, and to standardize the technology and improve the troughput by developing a software to automate the data analysis. Fields of science natural sciencescomputer and information sciencesdata sciencenatural sciencescomputer and information sciencessoftwarenatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsmicroscopy Programme(s) HORIZON.1.1 - European Research Council (ERC) Main Programme Topic(s) ERC-2022-POC1 - ERC PROOF OF CONCEPT GRANTS1 Call for proposal ERC-2022-POC1 See other projects for this call Funding Scheme HORIZON-AG-LS - HORIZON Lump Sum Grant Coordinator UNIVERSITAET HAMBURG Net EU contribution € 150 000,00 Address Mittelweg 177 20148 Hamburg Germany See on map Region Hamburg Hamburg Hamburg Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
