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Reading DNA in real time for medical applications

Project description

Real-time DNA recognition mapping developed

Surveillance of various diseases like cancer can be time consuming and expensive. Securing a faster, less- invasive method for monitoring them would be advantageous. The EU-funded BIoRead project has set its sights on developing nano-devices to analyse DNA single molecules for liquid biopsy. ‘Laser-Assisted DNA Optical Mapping’ (LADOM) is the result — a method that can detect DNA molecules from a sample in real time. LADOM yields quick and simple DNA analysis and detects tiny DNA fragments with less than a microlitre of fluid used. By leveraging this high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications, the project aims to standardise the technology and bring it to market.

Objective

The aim of this project is to use an ultra-fast, high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications. The proposed methodology, “Laser-Assisted DNA Optical Mapping (LADOM)”, allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device. It combines a cheap device fabrication, flexible DNA labelling (customizable for different applications), microscope- and camera-free set up, a read-out sensitive to single molecules, very high throughput (tens of molecules per minute), ability to detect very small fragments, and no limitation for the maximum molecule’s length, what is especially interesting for applications dealing with ultra-long intact, genomic DNA.
In this project, we request funding to offer a service to analyze samples from possible stakeholders from the biomedical sector, and to standardize the technology and improve the troughput bThe aim of this project is to use an ultra-fast, high throughput method for analysing DNA single molecules for liquid biopsy and other biomedical applications. The proposed methodology, “Laser-Assisted DNA Optical Mapping (LADOM)”, allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device. It combines a cheap device fabrication, flexible DNA labelling (customizable for different applications), microscope- and camera-free set up, a read-out sensitive to single molecules, very high throughput (tens of molecules per minute), ability to detect very small fragments, and no limitation for the maximum molecule’s length, what is especially interesting for applications dealing with ultra-long intact, genomic DNA.
In this project, we request funding to offer a service to analyze samples from possible stakeholders from the biomedical sector, and to standardize the technology and improve the troughput by developing a software to automate the data analysis.

Keywords

Host institution

UNIVERSITAET HAMBURG
Net EU contribution
€ 150 000,00
Address
MITTELWEG 177
20148 Hamburg
Germany

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Region
Hamburg Hamburg Hamburg
Activity type
Higher or Secondary Education Establishments
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Total cost
No data

Beneficiaries (1)