Supervised morphogenesis in gastruloids

Información del proyecto

SUMO

Identificador del acuerdo de subvención: 101071203

DOI

10.3030/101071203

Fecha de la firma de la CE 16 Septiembre 2022

Fecha de inicio 1 Noviembre 2022

Fecha de finalización 31 Octubre 2027

Financiado con arreglo a

• The European Innovation Council (EIC)

Coste total

€ 3 438 217,68

Aportación de la UE

€ 3 337 725,00

Coordinado por

OSLO UNIVERSITETSSYKEHUS HF
Norway

Periodic Reporting for period 2 - SUMO (Supervised morphogenesis in gastruloids)

Período documentado: 2023-11-01 hasta 2025-02-28

Resumen del contexto y de los objetivos generales del proyecto

Developing laboratory models that mimic organ functions are essential for advancing biomedical research and for reducing reliance on animal testing. Our project develops technologies that - based on early embryonic induction and vascularization - aims to generate embryonic organ structures.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

RP1: The SUMO consortium has developed advanced models for mouse gastruloid differentiation with a focus on conditions for the induction of the gut primordium induction and the heart field. An IT infrastructure for data sharing was set up, and machine learning was applied to predict gastruloid development. The consortium has initiated work towards embedding ethics to guide ethical and regulatory responsible work with human stem cell derived models.

RP2: The work in the second reporting period continues based on the achivements of RP1: Progress across work packages includes:
WP1: We developed stable mouse gastruloids with controlled lineage specification and a biobank of mESC lines.
WP2: We work towards a Raman analysis pipeline for tracking gastruloid development.
WP3: We developed a first iteration of machine learning classifiers to predict gastruloid development.
WP4: We work towards a vascularization platforms for gastruloids.
WP5: We work towards a human gastruloid differentiation protocol.
WP6: We work towards a closed-loop system for real-time gastruloid monitoring.
WP7: We work towards toxicology tests on gastruloids.
WP8: We work towards establishing an ethical and regulatory framework, engaging the research community.
WP9: We ensured effective communication and adherence to timelines.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

The SUMO project is advancing gastruloid models (3D stem cell-derived models) that replicate early embryonic development. These models have the potential to revolutionize developmental biology, toxicology, and regenerative medicine. Fundamental advances were made to create robust anteriorized and ventralized mouse and human gastruloid models that show evidence for the inductive areas that are essential for early organogenesis. The fundament for a self-guided vascularization of these structures is currenly explored.