Supervised morphogenesis in gastruloids

Projektinformationen

SUMO

ID Finanzhilfevereinbarung: 101071203

DOI

10.3030/101071203

EK-Unterschriftsdatum 16 September 2022

Startdatum 1 November 2022

Enddatum 31 Oktober 2027

Finanziert unter

• The European Innovation Council (EIC)

Gesamtkosten

€ 3 438 217,68

EU-Beitrag

€ 3 337 725,00

Koordiniert durch

OSLO UNIVERSITETSSYKEHUS HF
Norway

Periodic Reporting for period 2 - SUMO (Supervised morphogenesis in gastruloids)

Berichtszeitraum: 2023-11-01 bis 2025-02-28

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

Developing laboratory models that mimic organ functions are essential for advancing biomedical research and for reducing reliance on animal testing. Our project develops technologies that - based on early embryonic induction and vascularization - aims to generate embryonic organ structures.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

RP1: The SUMO consortium has developed advanced models for mouse gastruloid differentiation with a focus on conditions for the induction of the gut primordium induction and the heart field. An IT infrastructure for data sharing was set up, and machine learning was applied to predict gastruloid development. The consortium has initiated work towards embedding ethics to guide ethical and regulatory responsible work with human stem cell derived models.

RP2: The work in the second reporting period continues based on the achivements of RP1: Progress across work packages includes:
WP1: We developed stable mouse gastruloids with controlled lineage specification and a biobank of mESC lines.
WP2: We work towards a Raman analysis pipeline for tracking gastruloid development.
WP3: We developed a first iteration of machine learning classifiers to predict gastruloid development.
WP4: We work towards a vascularization platforms for gastruloids.
WP5: We work towards a human gastruloid differentiation protocol.
WP6: We work towards a closed-loop system for real-time gastruloid monitoring.
WP7: We work towards toxicology tests on gastruloids.
WP8: We work towards establishing an ethical and regulatory framework, engaging the research community.
WP9: We ensured effective communication and adherence to timelines.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The SUMO project is advancing gastruloid models (3D stem cell-derived models) that replicate early embryonic development. These models have the potential to revolutionize developmental biology, toxicology, and regenerative medicine. Fundamental advances were made to create robust anteriorized and ventralized mouse and human gastruloid models that show evidence for the inductive areas that are essential for early organogenesis. The fundament for a self-guided vascularization of these structures is currenly explored.