Objective
At the onset of mammalian life, the first lineage specification decision is made where embryonic cells opt to
become either part of the placenta or the future body. Proper regulation of this choice is crucial for subsequent
development. However, we dont know how the transcription program and 3D genome architecture arise,
interconnect, and are controlled to determine the fate of each cell in a developing embryo. Recent studies,
including my work, suggest that in addition to canonical mRNA-coding genes, RNAs from the dark parts of
the genome (e.g. transposons, repeats, long-non-coding RNAs) play a significant role during these events, yet
how specific classes of RNA regulate gene expression and nuclear architecture post fertilization remains
elusive. The main aim of my proposal is to understand the interrelationship between 3D genome organization
and the transcriptome across early development and to identify novel factors that lead to the first cell-fate
decision and concomitant decrease in cell potency.
Recent technical advances, which I co-developed, enabled simultaneous measurement of 3D genome
organization and the transcriptome, and facilitated large-scale functional screens in early mammalian embryos.
Thus, I now propose to generate spatiotemporal maps of 3D DNA and RNA organization from early mouse
embryos at single-cell resolution (Obj.1) to build a complete picture of the relationships between nuclear
architecture and emerging cell-type specific transcriptome that drive early cell fate choices. I will combine
these data with large-scale in vivo perturbations targeting protein coding genes (Obj.2) and dark genome
RNAs (Obj.3) to identify key inducers/regulators of lineage specification and to determine molecular
mechanisms governing cell-state transitions. The proposed research will help us to control, correct, and
eventually employ early stages of embryonic development and their high cell potency in vitro in reproductive
medicine and stem cell research.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences clinical medicine obstetrics
- natural sciences biological sciences genetics RNA
- medical and health sciences clinical medicine embryology
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2022-STG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1030 WIEN
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.