Project description
Advanced native mass spectrometry for analysis of non-covalent protein mixtures
Native mass spectrometry (NMS) analyses macromolecules, such as intact proteins and protein complexes, and enables the characterisation of the secondary, tertiary and quaternary structures of biomolecules. The EU-funded 2D-TOPMASS project aims to utilise Fourier transform ion cyclotron resonance mass spectrometry (MS) coupled to ultraviolet and infrared laser ion activation techniques to expand NMS application to large protein- and protein/nucleic-acid complexes. The application of NMS in combination with two-dimensional MS acquisition schemes will be tested for the analysis of non-covalent protein complexes and heterogeneous mixtures. The project will advance the application of NMS as a powerful technique for the study of protein complexes and assemblies in a broad range of samples.
Objective
Native mass spectrometry (MS) is a powerful structural biology tool to study protein complexes, their dynamics, assembly and function. In its so-called top-down approach, it aims to link specific proteoforms to higher assemblies such as non-covalent complexes, in which they function naturally and/or in a disease. The proposed project will leverage the power and versatility offered by state-of-the-art Fourier transform ion cyclotron resonance (FTICR) mass spectrometry coupled to ultraviolet and infrared laser ion activation techniques to advance native top-down MS of large protein- and protein / nucleic acid complexes. Studying a range of protein complexes up to small viral capsids, the project will push the boundaries of FTICR MS in the analysis of high-mass protein complex samples. Utilizing both standard commercially available protein complexes for method setup and benchmarking as well as biologically-relevant FOXO, TEAD and nucleosome protein / DNA complexes from the host laboratory, advanced laser- and electron-induced dissociation and fragmentation techniques and their combinations will be tested to obtain the best possible information on multiple levels of the complexes’ structure. Finally, the hitherto untried combination of native MS of non-covalent protein complexes with two-dimensional MS acquisition schemes will be explored to probe all species in a heterogeneous mixture at the same time. Linking the obtained sequential information on proteoforms to their complex and subcomplex stoichiometry, this approach will help increase the utility of native MS as a powerful technique for the study of proteoforms and protein assemblies applicable to a broad range of biologically / medically relevant samples promoting and reinforcing the position of native MS in the modern integrative structural biology portfolio as a technique providing information complementary to (and difficult to access by) classical high-resolution approaches.
Fields of science
- natural sciencesphysical sciencestheoretical physicsparticle physicsparticle accelerator
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- natural scienceschemical sciencesanalytical chemistrymass spectrometry
- natural sciencesbiological sciencesmolecular biologystructural biology
Programme(s)
Funding Scheme
HORIZON-AG-UN - HORIZON Unit GrantCoordinator
142 00 Praha 4
Czechia