Descripción del proyecto
Estudio sobre la cinética de crecimiento de los cultivos microbianos mixtos
La teoría de los estados de transición microbiana (MTS, por sus siglas en inglés) ofrece un modelo matemático para describir el crecimiento microbiano, que vincula los equilibrios termodinámicos y las tasas de crecimiento. Sin embargo, esta nueva teoría no puede describir suficientemente los cultivos microbianos mixtos que se encuentran en los sistemas de tratamiento de aguas residuales. El equipo del proyecto MTSonNAS, financiado con fondos europeos, utilizará la MTS para estudiar la cinética de crecimiento de dos bacterias nitrificantes diferentes. También investigará la inhibición del crecimiento bacteriano con diferentes tipos de inhibidores para probar la MTS para diferentes cohortes en cultivos microbianos mixtos en diferentes condiciones ambientales. Los resultados del proyecto permitirán mejorar la comprensión de los procesos de crecimiento microbiano en los sistemas de tratamiento naturales y artificiales.
Objetivo
Traditional engineering models known as Activated Sludge Model (ASM) are commonly used for operation, design, and optimization of wastewater treatment plants. ASM predict microbial growth with simulation and calibration of model parameters which are bound to a range of experimental conditions. Recently, Microbial Transition State (MTS) approach was proposed which is a statistical-physics based theory of microbial growth linking thermodynamic balances and growth rates. A growth equation was derived from these first principles, which links a flux (the growth of microbes) to a force, i.e. the Gibbs free potential energy density in medium contained in randomly distributed substrate molecules. MTS has not been fully verified with experimental data on mixed microbial cultures such as sustained in wastewater treatment systems. As the theory is developed for describing standard microbial growth, conditions such as inhibition of growth by any pollutant compound has not been considered/applied in real systems. The aim of the research is to investigate growth kinetics of two different nitrifying bacteria individually (Ammonia and Nitrite oxidizers) with MTS modeling approach and identify nitirification growth kinetics. Inhibition of bacterial growth with different types of inhibitors will also be investigated in order to evaluate and verify application of MTS model for different guilds in mixed microbial cultures under different environmental conditions. The outcomes of the study will contribute to understanding and control of microbial growth processes in engineered and natural treatment systems. Identification of inhibition kinetics will be used for mitigation of pollution generated from industrial processes as well as theoretical development of MTS theory to be applied in more complex biological process applications. The project is an intersection of environmental engineering, molecular biology, and modeling disciplines which will contribute Green Deal Strategy of EU.
Ámbito científico
- engineering and technologyenvironmental engineeringwater treatment processeswastewater treatment processes
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesearth and related environmental sciencesenvironmental sciencespollution
- natural sciencesbiological sciencesmolecular biology
Programa(s)
Convocatoria de propuestas
HORIZON-WIDERA-2022-TALENTS-02
Consulte otros proyectos de esta convocatoriaRégimen de financiación
HORIZON-AG-UN - HORIZON Unit GrantCoordinador
34469 Maslak, Istanbul
Turquía