Objective
The goal of biological research is to understand how life works. Although progress is fast, there seems to be an infinity of things we do not understand. When it comes to understanding tissue from the bottom up, our knowledge leaves much to desire. Feynman claimed that “It is very easy to answer many of these fundamental biological questions; you just look at the thing!” well the problem is that looking at the thing is the problem. Microscopy might never give us the possibility to directly see DNA- or RNA-sequence. For this, the community has evolved extraordinarily powerful sequencers. Today one man can routinely read millions of sequences on a weekly basis. And likely soon, we will read billions of sequences daily in small labs. But this, in itself, will not allow us to just look at the thing. We argue in this proposal, that by using the sequencer itself as a microscope, we will get that much closer to actually see what is going on in biological systems.
Researchers have started in this direction by coupling microscopy- and sequencing-data from the same sample, but that is a temporary solution. Here, we propose a technology for inferring images using sequencing data alone, bypassing the need for advanced microscopy and leveraging the potential of the exponential growth of sequencing technology.
We use DNA seeds and perform a reaction in-situ that allow these seeds to copy themselves locally. This is analogous to phylogenetic reconstruction, but instead of inferring ancestry, we infer relations of amplicons to spatial locations in tissue. By using a unique approach, we derive spatial information connected to RNA transcript information directly in-situ, allowing for a non-targeted spatial transcriptomics technique that is as simple as running a PCR. When successful, this approach will then enable us, and others, to learn the inner secrets of biological system at a significantly faster rate.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences genetics RNA
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2022-ADG
See all projects funded under this callHost institution
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
171 77 STOCKHOLM
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.