Descripción del proyecto
Aislamiento de exosomas con ultrasonidos
La separación acústica de células se basa en el uso de ultrasonidos para clasificar células con diferentes propiedades físicas, como su tamaño o su densidad. El equipo del proyecto AcouSome, financiado por el Consejo Europeo de Innovación, propone emplear ultrasonidos para separar exosomas. Los exosomas son vesículas extracelulares rodeadas por una membrana que transportan proteínas, lípidos y ácidos nucleicos, y funcionan como un medio de comunicación entre las células y los tejidos. Teniendo en cuenta el papel emergente de los exosomas en diferentes enfermedades, el objetivo general de AcouSome es desarrollar un método para el aislamiento rápido y fácil de exosomas con fines de investigación y diagnóstico.
Objetivo
AcouSome is a consortium stemming from the ongoing EIC-FET Open BioWings project, and consists of four of the BioWings project
partners (AcouSort AB, DTU, Lund University and Day One Srl). AcouSome has the ambition to build on the disruptive technology of
thin film actuated acoustofluidics developed in BioWings. The results obtained in BioWings will be combined with a polymer
acoustofluidics technology developed in the ongoing Eurostars AcouPlast project to fabricate a polymer-based microfluidic chip for
separating and enriching exosomes from blood for use in the next generation of point-of-care diagnostics.
Exosomes are nano-sized extracellular vesicles that are released by a significant number of different cell types. They are considered an
important biomarker, with high diagnostic potential in a wide range of diseases, including different kinds of cancer (glioblastoma,
melanoma, prostate cancer and many others), hepatitis, kidney, cardiovascular and liver diseases. Therefore, there is an increasing
interest in exosomes as a powerful diagnostic tool. Having the opportunity to isolate and analyze exosomes from a routine blood test
is crucial for early detection of a wide range of diseases.
In the AcouSome chip exosomes will be separated and enriched from blood by combining two steps already developed by Lund
University and AcouSort. First plasma will be separated from blood by flowing the blood through a microfluidic channel, pushing the
cells towards the centerline of the channel using ultrasoundand and subsequently splitting the cell and plasma flows. Exosomes will
then be trapped and enriched from the plasma flow using a localized acoustic field. The acoustofluidic chip will be driven using
thin-film actuators as invented together with DTU in the BioWings project. The exosome separation cartridge is intended for sample
preparation in research labs and future diagnostic point-of-care instruments.
Ámbito científico
- natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidics
- engineering and technologyother engineering and technologiesmicrotechnologylab on a chip
- engineering and technologymedical engineeringmedical laboratory technologydiagnostic technologies
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectrical engineeringpiezoelectrics
- natural sciencesphysical sciencesacousticsultrasound
Palabras clave
Programa(s)
- HORIZON.3.1 - The European Innovation Council (EIC) Main Programme
Convocatoria de propuestas
HORIZON-EIC-2022-TRANSITION-01
Consulte otros proyectos de esta convocatoriaRégimen de financiación
HORIZON-EIC - HORIZON EIC GrantsCoordinador
223 81 Lund
Suecia
Organización definida por ella misma como pequeña y mediana empresa (pyme) en el momento de la firma del acuerdo de subvención.