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Development & characterisation of a pre-vascularised tubular scaffold for tracheal regeneration that supports the formation of an iPSC-derived epithelium

Project description

Tracheal restoration: 3D printed tubular scaffolds supporting new cell growth

The trachea, also known as the windpipe, conveys air to and from the lungs. The trachea is a large tube lined with cells that produce mucous; it is reinforced by C-shaped cartilage rings that flexibly support it. Tracheal damage has become more common due to mechanical intubation related to COVID, and damage in general can be life-threatening. Currently, implants to repair severe damage weaken over time and do not support the formation of new tissues. With the support of the Marie Skłodowska-Curie Actions programme, the iTRACH project seeks to address this by developing and characterising 3D-printed cell-based pre-vascularised tubular scaffolds designed to mimic tracheal biomechanics and support the growth of tracheal tissues.

Objective

Tracheal damage is often life-threatening, and its incidence has increased due to invasive mechanical intubation of COVID-19 patients. Short defects undergo a surgical procedure (anastomosis), but this is not feasible for extensive defects. While bioengineered approaches for such defects have been investigated, there has been little translation into the clinic as implants weaken over time and fail to support the formation of neotracheal tissues. I aim to solve this problem by developing and characterising 3D printed tubular scaffolds for use as medical devices for tracheal restoration via a Global Fellowship involving Royal College of Surgeons in Ireland and Boston University, followed by a non-academic placement in CellInk. Scaffolds will be designed to mimic tracheal biomechanics and will be extensively characterised. Induced pluripotent stem cells (iPSCs) will be used to develop a pre-vascularised mature respiratory epithelium on disc scaffolds in combination with endothelial progenitors and human mesenchymal stem cells (hMSCs). The tri-culture will then be moved into the tubular design and the pre-vascularised mucosal substitute will be grown and assessed in vitro as an approach for ex vivo seeding of tracheal grafts prior to implantation. The ability of the tubular scaffold to support the growth of cartilage tissues will be assessed using a combination of bioprinting and differentiation of hMSCs. iTRACH will provide me with crucial training in advanced research skills (iPSCs, stem cell biology, biomaterials & 3D printing) and transferrable skills (Intellectual Property, Project Management, Entrepreneurship, Professional Enhancement and Science Communication). This fellowship will provide me with the required technical and complementary skills to enhance my employability and to allow me to transition into a research position in a leading biomedical company, while also allowing for the possibility of me staying in an academic role with links to industry.

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HORIZON-TMA-MSCA-PF-GF - HORIZON TMA MSCA Postdoctoral Fellowships - Global Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2022-PF-01

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Coordinator

ROYAL COLLEGE OF SURGEONS IN IRELAND
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 328 494,96
Address
ST STEPHEN'S GREEN 123
2 DUBLIN
Ireland

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Activity type
Higher or Secondary Education Establishments
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Total cost

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