Hydrodynamics of Double Membrane Spreading in Biology

Projektinformationen

HYDROBIOMEM

ID Finanzhilfevereinbarung: 101106384

DOI

10.3030/101106384

Das Projekt endete am 30 April 2024

EK-Unterschriftsdatum 6 Juli 2023

Startdatum 1 September 2023

Enddatum 31 August 2025

Finanziert unter

• Marie Skłodowska-Curie Actions (MSCA)

Gesamtkosten

Keine Daten

EU-Beitrag

€ 210 911,04

Koordiniert durch

UNIVERSITETET I OSLO
Norway

Periodic Reporting for period 1 - HYDROBIOMEM (Hydrodynamics of Double Membrane Spreading in Biology)

Berichtszeitraum: 2023-09-01 bis 2025-08-31

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

In HYDROBIOMEM I have worked to develop novel theoretical tools and computational methods to describe the double membrane wrapping in autophagy by combining tools from soft matter physics, differential geometry and fluid mechanics. Autophagy is a dynamic biomembrane process that is the principle pathway for calls to degrade harmful components. As such, its misregulation is involved in an enormous number of pathologies including cancer, Alzheimers and Parkinson’s. Here we aim to develop a biophysical understanding of the fundamental processes which govern the membrane dynamics of autophagy, in particular the role of activity via the recruitment of binding proteins and lipids, and hydrodynamic dissipation.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

Note that this project was ended early for personal reasons. I developed general covariant hydrodynamic equations to describe the curvature coupling and dissipative dynamics lipid membranes with proteins embedded across the membrane. This complemented earlier work performed in the group analyzing the statics of such a system in the geometry of a tube.

I developed a numerical scheme for solving 1D geometric PDEs in arbitrary geometry, initially this was applied to a simplified system of odd active filaments. The long-term goal is to apply such methods to axis-symmetric double membrane wrapping problems.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The development of protein-membrane covariant hydrodynamic equations in general, coordinate free form was an advance beyond the current state of the art. In addition the application of such equations to the instability of a protein covered membrane tube yielded new insights on the dissipative mechanisms governing the protein-induced pearling instability. We showed that the membrane viscosity is the dominant dissipative factor in the secondary dispersive peak (short wavelength instability).